Phospholipid composition of gliding bacteria: oral isolates of Capnocytophaga compared with Sporocytophaga

Abstract

The distribution of acetone-soluble (neutral glycolipid) and acetone-insoluble (phospholipid isoprenoids) lipids in oral isolates of gram-negative gliding bacteria of the genus Capnocytophaga was compared with those in a non-host-related gliding bacterium, Sporocytophaga myxococcoides. The acetone-soluble material accounted for 34 to 55% of the extracted lipids; the remainder was acetoneinsoluble material. The major phospholipid was phosphatidylethanolamine (67%), with lesser amounts of lysophosphatidylethanolamine and several unidentified phosphate-containing compounds. Capnocytophaga also contained significant amounts of an ornithine-amino lipid.

The phospholipid components of bacteria related to periodontitis.

“…The quantitative analysis of phospholipids was performed with the use of a Chromarod thin-layer chromatographic flame-ionization detection system, because of its reliability, reproducibility and sensitivity [22,23]. The phospholipid components revealed in this study were similar to those found in another previous study which analyzed their respective quantities on the basis of weight percentage [9][10][11][24][25][26][27].…”

“…In Capnocytophaga sp., no L-PE was identified in this study. However, HOLT et al [10] found PE (62-67%), L-PE (10-14%) and other unidentified substances in this species.…”

“…, Capnocytophaga sputigena, Capnocytophaga ochracea, Capnocytophaga gingivalis [10] and Bacteroides melaninogenicus [11]. Table 3.…”

Cellular distribution and linkage of D-(-)-3-hydroxy fatty acids in Bacteroides species

“…Another possible nitrogen donor, for both lipid and bound amide-linked fatty acids, is ornithine or a similar dibasic amino acid. Ornithine-containing lipids have been demonstrated in several bacterial species (6,8,17,26,28). In these compounds, the amide-linked fatty acid is hydroxylated, with another fatty acid, either hydroxy or nonhydroxy, in ester linkage via the 3-OH group.…”

Hydroxy fatty acids in Bacteroides species: D-(--)-3-hydroxy-15-methylhexadecanoate and its homologs

“…Holt et al (5) do not report such compounds in Sporocytophaga and Capnocytophaga, but these organisms may also contain hydroxy acids. Since some members of the genus Capnocytophaga have until recently been included in the genus Bacteroides (10), it is possible that some of the unidentified phospholipids reported by Holt et al (4) are phospho-phingolipids as reported in Bacteroides species by several groups (3, 6-8, 23, 24).…”