Phospholipid composition of mitochondria and microsomes from regenerating rat liver and hepatomas of different growth rate

Bergel'son, L. D.; Dyatlovitskaya, É. V.; Sorokina, I B; Gorkova, N.P.

doi:10.1016/0005-2760(74)90067-8

Cited by 46 publications

(17 citation statements)

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“…Since mitochondrial and microsomal fractions from hepatoma cells had a phos pholipid class composition that was rather similar to the corresponding fractions from normal liver, these changes in phospholipid composition may be characteristic features of hepatoma cell PM. These results are not completely consistent with the findings of Bergelson et al (2)(3)(4) that the characteristic phospholipid pattern found in the subcel lular organelles of normal liver was not observed in organelles isolated from hepatoma cells.…”

Section: Resultscontrasting

confidence: 86%

Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Koizumi

Tamiya‐Koizumi

Fujii

et al. 1977

Cell Struct. Funct.

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ABSTRACT.Phospholipid analyses were made on whole cells and isolated plasma membranes (PM) of six different lines of Yoshida ascites hepatoma and normal liver. The hepatoma PM had lower percentage levels of choline phosphoglycerides and phosphatidylinositol, and higher percentages of sphingomyelin, phosphatidylethanolamine and phosphatidylserine in total phospholipids than liver PM. Similar tendencies were observed in the whole cell phospholipids, although such trends were not observed in the mitochondrial or microsomal fractions. The lysophospholipid percentages in hepatoma PM were lower than those in liver PM. Hepatomas had higher percentage levels of plasmalogen in total phospholipids than normal liver, in both whole cells and PM. PM from island-forming hepatomas contained more plasmalogen than PM from their free-cell sublines. Localization of plasmalogen was observed in PM and certain intracellular organelles of island-forming hepatomas by histochemical plasmal reaction.

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Section: Resultscontrasting

confidence: 86%

Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Koizumi

Tamiya‐Koizumi

Fujii

et al. 1977

Cell Struct. Funct.

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“…Although it is well established that protein synthesis is increased during the period of re-growth, the details of the reconstitution of lipid components (membranes, fat-droplets, lipoproteins) are not fully documented. There is usually an increased content of hepatic fat during regeneration; the extra lipid consists of triacylglycerols (Glende & Morgan, 1968), whereas phospholipid deposition (with the exception of cardiolipin) exactly keeps pace with the overall growth rate (Glende & Morgan, 1968;Tata, 1970;Fex,, 1970b;Bergelson et al, 1974;Fex & Thorzell, 1975). Triacylglycerol synthesis, at least from preformed fatty acids, is increased after resection of part of the liver, as may be demonstrated with labelled fatty acids (Fex & Olivecrona, 1968a;Olivecrona & Fex, 1970;Fex, 1970a) or glycerol (Olivecrona & Fex, 1970).…”

mentioning

confidence: 95%

Fatty acid synthesis in the regenerating liver of the rat

Gove¹,

Hems²

1978

Biochemical Journal

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1. Synthesis de novo of fatty acids in the rat liver, measured per g wet wt. of tissue, was increased by a factor of about two, between 1 and 4 days after partial hepatectomy, compared with rates in sham-operated control rat livers. 2. There were no associated changes in the rates ofliver cholesterol synthesis or of adipose-tissue fatty acid synthesis in rats after partial hepatectomy, compared with rates in sham-operated rats. 3. In regenerating livers, perfused under three different conditions, there was no alteration in the capacity for fatty acid synthesis compared with that of control rats. 4. The increased synthesis of fatty acids in regenerating liver was associated with insignificant increases in plasma concentrations oftriacylglycerols and free fatty acids, with a decrease in content of liver glycogen, and with no change in hepatic activity of acetyl-CoA carboxylase. 5. The accelerated rate ofsynthesis offatty acids in regenerating liver appears not to be due to any intrinsic alteration in hepatic capacity for fatty acid synthesis, but it may be caused by the continuous action on liver of unidentified circulating factors.

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“…Numerous structural and biochemical abnormalities occur in tumor cell mitochondria that could compromise function, thus forcing a reliance on glycolysis for cell survival (5,6,9,(15)(16)(17). Although several prior studies have evaluated the lipid composition of tumor mitochondria (18)(19)(20)(21)(22)(23)(24)(25), no prior studies have evaluated both the content and the composition of cardiolipin (CL) in highly purified mitochondria isolated from brain tumors and from their orthotopic host tissue.CL (1,3-diphosphatidyl-sn -glycerol) is a complex mitochondrial-specific phospholipid that regulates numerous enzyme activities, especially those related to oxidative phosphorylation and coupled respiration (26-31). CL binds complexes I, III, IV, and V and stabilizes the super complexes (I/III/IV and II/III/IV), demonstrating an absolute requirement of CL for catalytic activity of these enzyme complexes (27,29,32,33).…”

mentioning

confidence: 99%

mentioning

confidence: 99%

Cardiolipin and electron transport chain abnormalities in mouse brain tumor mitochondria: lipidomic evidence supporting the Warburg theory of cancer

Kiebish

Han

Cheng

et al. 2008

Journal of Lipid Research

253

230

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Otto Warburg first proposed that cancer originated from irreversible injury to mitochondrial respiration, but the structural basis for this injury has remained elusive. Cardiolipin (CL) is a complex phospholipid found almost exclusively in the inner mitochondrial membrane and is intimately involved in maintaining mitochondrial functionality and membrane integrity. Abnormalities in CL can impair mitochondrial function and bioenergetics. We used shotgun lipidomics to analyze CL content and composition in highly purified brain mitochondria from the C57BL/6J (B6) and VM/Dk (VM) inbred strains and from subcutaneously grown brain tumors derived from these strains to include an astrocytoma and ependymoblastoma (B6 tumors), a stem cell tumor, and two microgliomas (VM tumors). Major abnormalities in CL content or composition were found in all tumors. The compositional abnormalities involved an abundance of immature molecular species and deficiencies of mature molecular species, suggesting major defects in CL synthesis and remodeling. The tumor CL abnormalities were also associated with significant reductions in both individual and linked electron transport chain activities. A mathematical model was developed to facilitate data interpretation. Otto Warburg first proposed that the prime cause of cancer was impaired energy metabolism (1, 2). This impairment involved irreversible injury to cellular respiration that was followed in time by a gradual dependence on fermentation (glycolytic) energy to compensate for the energy lost from respiration. Cell viability requires a constant delta G′ of ATP hydrolysis of approximately 257 kJ/mol (3, 4). Most normal mammalian cells achieve this level of useable energy through respiration, whereas tumor cells achieve this level through a combination of respiration and glycolysis (2, 5). Indeed, elevated glycolysis is the metabolic hallmark of nearly all tumors, including brain tumors, and is the basis for tumor imaging using labeled glucose analogs (5-8). Much controversy has surrounded the Warburg theory, however, largely over issues regarding the Pasture effect and aerobic glycolysis (9-14). Numerous structural and biochemical abnormalities occur in tumor cell mitochondria that could compromise function, thus forcing a reliance on glycolysis for cell survival (5,6,9,(15)(16)(17). Although several prior studies have evaluated the lipid composition of tumor mitochondria (18)(19)(20)(21)(22)(23)(24)(25), no prior studies have evaluated both the content and the composition of cardiolipin (CL) in highly purified mitochondria isolated from brain tumors and from their orthotopic host tissue.CL (1,3-diphosphatidyl-sn -glycerol) is a complex mitochondrial-specific phospholipid that regulates numerous enzyme activities, especially those related to oxidative phosphorylation and coupled respiration (26-31). CL binds complexes I, III, IV, and V and stabilizes the super complexes (I/III/IV and II/III/IV), demonstrating an absolute requirement of CL for catalytic activity of these enzyme comp...

show abstract

Phospholipid composition of mitochondria and microsomes from regenerating rat liver and hepatomas of different growth rate

Cited by 46 publications

References 2 publications

Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Phospholipids of Plasma Membranes Isolated from Rat Ascites Hepatomas and from Normal Rat Liver

Fatty acid synthesis in the regenerating liver of the rat

Cardiolipin and electron transport chain abnormalities in mouse brain tumor mitochondria: lipidomic evidence supporting the Warburg theory of cancer

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