Phospholipid composition of the inner mitochondrial membrane of rat hepatocytes upon development of different types of steatohepatosis

Abstract

Nonalcoholic fatty liver disease (NAFLD) or steatohepatosis has recently become widespread, but its pathogenesis has not been thoroughly understood for today. Most scientists have appropriated a central role in the mechanisms of its development to mitochondria and so-called "mitochondrial dysfunction," which is observed in most animal models and in most patients. The aim of this work was to determine phospholipid composition of inner mitochondrial membrane of rat hepatocytes under diet-induced and glutamate-induced steatohepatosis, as well as to compare the data about developing steatohepatosis of different types. Obtained data indicate the disruption of normal functional state of the inner mitochondrial membrane under the conditions of diet-induced and glutamate-induced steatohepatosis. Amount of oxidized forms of the major phospholipids including cardiolipin, indicates the increasing oxidative stress under the conditions of both steatohepatosis types.