Phospholipid-derived fatty acids as chemotaxonomic markers for phytoplankton: application for inferring phytoplankton composition

Dijkman, Nicole A.; Kromkamp, Jacco C.

doi:10.3354/meps324113

Cited by 129 publications

(145 citation statements)

References 37 publications

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“…Phytoplankton FA biomass was calculated from the sum of phytoplankton PLFAs ( 18:3ω3,18:4ω3,20:5ω3,22:6ω3,and 20:4ω6) and a C : specific FA ratio of 60 based on culture studies, summarized in Dijkman and Kromkamp (2006). The two approaches yielded similar results.…”

Section: δ 13 C Of Allochthonous Carbonmentioning

confidence: 61%

“…Poly-unsaturated fatty acids (PUFAs) are abundant in most phytoplankton, and can generally be used as chemotaxonomic markers for this group (Dijkman and Kromkamp, 2006). The most abundant PUFAs in all lakes were 18:3ω3 (α-linolenic acid), 18:4ω3 (stearidonic acid, SDA), 20:5ω3 (icosapentaenoic acid, EPA), 22:6ω3 (docosahexaenoic acid, DHA) and 20:4ω6 (arachidonic acid, ARA), common PUFAs in freshwater phytoplankton (Taipale et al, 2013), and their concentration-weighted δ 13 C were used to determine phytoplankton isotope ratios (δ 13 C phyto ).…”

Section: δ 13 C Of Phytoplankton and Bacteriamentioning

confidence: 99%

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Stable carbon isotope biogeochemistry of lakes along a trophic gradient

et al. 2014

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Abstract. The stable carbon (C) isotope variability of dissolved inorganic and organic C (DIC and DOC), particulate organic carbon (POC), glucose and polar-lipid derived fatty acids (PLFAs) was studied in a survey of 22 North American oligotrophic to eutrophic lakes. The δ 13 C of different PLFAs were used as proxy for phytoplankton producers and bacterial consumers. Lake pCO 2 was primarily determined by autochthonous production (phytoplankton biomass), especially in eutrophic lakes, and governed the δ 13 C of DIC. All organic-carbon pools showed overall higher isotopic variability in eutrophic lakes (n = 11) compared to oligomesotrophic lakes (n = 11) because of the high variability in δ 13 C at the base of the food web (both autochthonous and allochthonous carbon). Phytoplankton δ 13 C was negatively related to lake pCO 2 over all lakes and positively related to phytoplankton biomass in eutrophic lakes, which was also reflected in a large range in photosynthetic isotope fractionation (ε CO 2 −phyto , 8-25 ‰). The carbon isotope ratio of allochthonous carbon in oligo-mesotrophic lakes was rather constant, while it varied in eutrophic lakes because of maize cultivation in the watershed.

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Section: δ 13 C Of Allochthonous Carbonmentioning

confidence: 61%

Section: δ 13 C Of Phytoplankton and Bacteriamentioning

confidence: 99%

Stable carbon isotope biogeochemistry of lakes along a trophic gradient

et al. 2014

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“…4a). PLFAs 16:2v4, 16:3v4, 20:4v6, and 20:5v3, which are considered specific for diatoms (Dijkman and Kromkamp 2006), showed substantially higher Dd 13 C values for the old sediment than for the fresh sediment (Fig. 4a).…”

Section: Resultsmentioning

confidence: 99%

“…This is consistent with diatoms being the dominant algal group in the studied sediment. However, the most abundant PLFA in diatoms is 20:5v3 (Dijkman and Kromkamp 2006), while the 13 C-labeled profile was actually dominated by 16:0, 16:1v7c, and 18:1v7c. This was most likely the result of additional 13 C fixation by bacteria.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, the best available proxy for living diatoms in the present study is the concentration of PLFA 20:5v3 (Fig. 1), which is the dominant PLFA in diatoms (Dijkman and Kromkamp 2006). PLFAs are comparatively rapidly degraded upon cell death and are therefore often considered to be specific for living biomass (Boschker and Middelburg 2002).…”

Section: Discussionmentioning

confidence: 99%

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Long‐term pigment dynamics and diatom survival in dark sediment

Veuger

Oevelen

2011

Limnology & Oceanography

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In order to investigate survival of diatoms and long-term pigment dynamics in dark sediment, we incubated samples of homogenized, sieved, tidal-flat sediment for 1 yr in darkness. Microscopic observations revealed that some diatoms survived the full year in darkness and retained their pigments. Concentrations of the diatomspecific phospholipid-derived fatty acid (PLFA) 20:5v3 indicate that these survivors represented only a small fraction of the original diatom community, probably no more than a few percent. Consequently, the effect of pigment retention in living diatoms on the overall pigment dynamics was negligible. The photosynthetic potential of the surviving diatoms was further investigated by re-exposing the sediment to light in the presence of 13 Cbicarbonate. Rapid 13 C fixation into algal PLFAs at a level similar to that in sediment freshly collected from the field indicates that surviving diatoms fully retained their photosynthetic capacity. Concentration dynamics of photosynthetic pigments generally reflected degradation of two different pools with clearly different loss rates. Losses during the first 1-2 months of the experiment (half lives of 6-22 d) reflected degradation of fresh algal material, while losses during the rest of the experiment reflected much slower degradation of more refractory pigment pools (half lives of $ 1 yr). Individual pigments showed distinctly different labilities, with losses ranging from 0% to 86% over the full year. The order of overall loss was fucoxanthin . chlorophyll c . chlorophyll a . diadinoxanthin . pheophorbide . pheophytin . diatoxanthin.

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Integrating pigment and fatty acid profiles for enhanced estimation of seston community composition

Litmanen,

Perälä,

Vuorio

et al. 2024

Ecosphere

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Climate change, nutrition pollution, and land use alterations influence the primary production of lakes. While light‐microscopy counting remains the standard for estimating phytoplankton community composition, its expense and time‐consuming nature necessitate cost‐effective alternatives for seston analysis. Furthermore, estimating the contribution of seston constituents other than primary producers, or non‐algal particles, is not possible with light‐microscopy counting. Biotracer approach using computational methods and chemotaxonomic biomarkers such as carotenoid pigments and fatty acids have been used as an alternative in seston analysis when species‐level taxonomy is not required. However, a comprehensive testing of how well carotenoid and fatty acids can be used in estimating a wide range of seston phytoplankton communities using different estimation methods is lacking. To assess the accuracy of a suite of state‐of‐the‐art biotracer‐based computational methods, namely CHEMTAX, FASTAR (Fatty Acid Source‐Tracking Algorithm in R), MixSIAR, and QFASA (Quantitative Fatty Acid Signature Analysis), lake water samples were collected in 2016, 2018, 2019, 2020, and 2021 for seston composition analysis in a boreal eutrophic lake with light‐microscopy counting serving as the reference for seston composition. Absolute errors between the biotracer‐based estimates were calculated to evaluate method performance. A small laboratory experiment to assess the reliability of estimating the contribution of non‐algal particles using the computational methods with fatty acids was also conducted. The closest alignment to light‐microscopy counting in terms of absolute error was achieved when both carotenoids and fatty acids were used together in the QFASA method. For CHEMTAX, FASTAR, and MixSIAR, using carotenoids alone produced the closest results. Additionally, the estimation methods accurately assessed the proportion of non‐algal particles in the seston when using fatty acid profiles, a capability not possible with light‐microscopy counting. Our findings demonstrate that the biotracer approach provides a viable and cost‐effective alternative to light‐microscopy counting when group‐level information of phytoplankton community composition suffices. Furthermore, we show that non‐algal particles can be effectively estimated together with phytoplankton when using fatty acids.

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Phospholipid-derived fatty acids as chemotaxonomic markers for phytoplankton: application for inferring phytoplankton composition

Cited by 129 publications

References 37 publications

Stable carbon isotope biogeochemistry of lakes along a trophic gradient

Stable carbon isotope biogeochemistry of lakes along a trophic gradient

Long‐term pigment dynamics and diatom survival in dark sediment

Integrating pigment and fatty acid profiles for enhanced estimation of seston community composition

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