Phospholipid metabolism in the liver and lung of rats during development

Weinhold, Paul A.; Villee, Claude A.

doi:10.1016/0005-2760(65)90070-6

Cited by 90 publications

(44 citation statements)

References 21 publications

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“…The size of lung lecithin also increased with age, but the elevation was not so significant as compared with that of liver. The data were in close agreement with those of rat lung and liver reported by Weinhold and Villee (1965). On fatty acid composition, it was noted that palmitate was the principal fatty acid of lecithin in both tissues and it decreased in liver but increased in lung with age.…”

Section: Resultssupporting

confidence: 91%

“…The high ratio suggests that the reaction is principally operated by acyl-migration of two molecules of lysolecithin. metabolism of lecithin; dipalmitoyl lecithin A few reports have been presented on the composition and metabolism of liver phospholipids during development (Weinhold and Villee 1965;Weinhold 1969;Dobiasova et al 1964). Of lung phospholipids, the presence of an unusual phospholipid, dipalmitoyl lecithin, has stimulated considerable interest in lipid metabolism of the lung.…”

mentioning

confidence: 99%

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On the Metabolism of Lecithin in Lung and Liver of Fetal Rabbits

Abe

Akino

Ohno

1973

Tohoku J. Exp. Med.

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Section: Resultssupporting

confidence: 91%

mentioning

confidence: 99%

On the Metabolism of Lecithin in Lung and Liver of Fetal Rabbits

Abe

Akino

Ohno

1973

Tohoku J. Exp. Med.

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“…Measurements of phosphorus in crude surfactant, organic solvent soluble extracts, and Sephadex LH-20 eluates were camed out after Folch extraction (3 1) and quantified by the methods of Weinhold and Villee (32). Samples of LH-20 eluates for lipid analysis were dried under nitrogen and redissolved in small volumes of chloroform/methanol(2: 1, vol/vol).…”

Section: Efficacy Of Dpp( :/Spl5-26 Mixtures 485mentioning

confidence: 99%

Mixtures of Low Molecular Weight Surfactant Proteins and Dipalmitoyl Phosphatidylcholine Duplicate Effects of Pulmonary Surfactant in Vitro and in Vivo

Smith¹,

Taeusch²,

Phelps³

et al. 1988

Pediatr Res

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ABSTRACT. Pulmonary surfactant proteins SP-B and SP-C were isolated from lavage fluids of bovine lungs and recombined (lipid/proteins, 911, wt/wt) with dipalmitoyl phosphatidylcholine for testing in vitro and in surfactantdeficient adult rats. Using a pulsating bubble surfactometer, we found that inflation pressures of bubbles at minimum radii in these mixtures were 0.34 f 0.05 cm H 2 0 (+ SD, n = 24) after 1 min. These values were not affected by increasing amounts of surfactant protein relative to dipalmitoyl phosphatidylcholine (DPPC). Minimum inflation pressures were similar to those of modified bovine surfactant, surfactant Tokyo Akita (TA) (0.33 f 0.05 cm H20, n = 7). I n vivo testing was carried out in adult rats made surfactant deficient by repeated lavage and ventilated with 100% oxygen. Rats received tracheal instillations of either air, DPPC, DPPCISP-B,C (9:1), or surfactant TA at 50 mg/kg body weight. Surfactant TA and DPPCISP-B, SP-C mixtures resulted in similar immediate and sustained improvements in arterial oxygenation (308 + 66 torr, n = 10 and 312 2 101 torr, n = 6 at 30 min posttreatment) that were significantly greater than those of sham (76 f 24 torr, n = 17) and DPPC-treated rats (64 f 32 torr, n = 7). Rats treated with either DPPC/SP-B,C mixtures or surfactant TA showed similar postmortem static lung compliances (2.3 f 0.8 ml/cm H20/kg, n = 8 and 1.9 2 0.4 ml/cm H20/kg, n = 5, respectively) that were significantly larger than sham (1.3 + 0.3 ml/cm H 2 0 / kg, n = 14) and DPPC-treated rats (1.2 f 0.2 ml/cm H20/ kg, n = 6). We conclude that simple mixtures of DPPC

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“…Alterations in the rate of synthesis of PC may reflect alterations in the rate of synthesis of all cellular membranes, of which PC is a major component (22). The high incorporation of precursors into PC early in incubation, at days 12 and 14, is probably due to the active growth of the bronchial tubules and the blood capillaries at these stages.…”

Section: Discussionmentioning

confidence: 99%

Lung Development in the Chick Embryo. I. Phosphatidylcholine Synthesis in the Developing Chick Lung

Compton

Goeringer

1981

Pediatric Research

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SummaryWe investigated the relative contribution cf the two pathways of phosphatidylcholine (PC) synthesis in lungs from chick embryos of 12 to 20 days of incubation as well as from hatched chicks and adult chickens. Lung "blocks" were incubated for 60 min in vitro in the presence of IgHlmethionine and [14Clcholine. P C was s e p arated by thin-layer chromatography, and the amount of labeled product was determined by liquid scintillation counting. P C synthesis (expressed as pmoles P C per mg protein per 60 min) by the choline pathway declined from the 12-day value of 40 to a minimum of 8 at day 16 and then rose gradually to a peak of 74 on day 1 posthatching. P C synthesis via the phosphatidylethanolamine pathway also declined from day 12 (9) to day 16 (13) but peaked on day 19 (65) and again on day 1 posthatching (9). Synthetic activities of the two pathways differed significantly (P 5 0.05) only on days 14, 18, and 19 of incubation. Unlike the mammalian lung, neither pathway seems to predominate throughout incubation or after hatching. The increase in P C synthesis towards the end of incubation correlates with the time of appearance of lamellar bodies in the lung epithelial cells and of surfactant in the airways. SpeculationBecause normal morphologic and biochemical development of the chick lung resembles that occurring in the mammalian lung in many respects, conclusions drawn from the study of avian lungs may well be germane to an appreciation of mammalian lung differentiation in general. Information concerning regulation of the biosynthetic pathways for phosphatidylcholine in the avian embryo may provide some clues for improved clinical treatment of certain pulmonary disorders, including respiratory distress syndrome of the newborn.Until recently, the question of whether or not the avian lung possessed surfactant was a matter of controversy (17). Now this material has been demonstrated lining the parabronchial, atrial, and air capillary membranes in the lungs of several avian species (18. 19). Cells resembling mammalian types I and I1 pneumocytes have been described by several investigators in the adult and embryonic chick lung (14, 21).The major component of avian pulmonary surfactant is phosphatidylcholine (PC) (16,21). Two pathways are involved in the de novo synthesis of PC: the choline incorporation pathway (I) and the phosphatidylethanolamine (PE) methylation pathway (11). Fetal and adult lungs of all mammalian species examined to date have been shown to possess the enzymes necessary for each pathway (1,6,7). ~h e ' c h o l i n e incorpdration has been identified as the maior route for PC svnthesis in the adult and late fetal mammalian l A g (5). No studieg have been published on the pathways for PC synthesis in the embryonic, newly hatched, or adult chicken lung.We investigated the production of PC in blocks of lungs from chicks of different ages. The blocks were incubated in the presence of radioactive precursors specific to the two pathways for PC synthesis. This provided us with a measure of the r...

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Phospholipid metabolism in the liver and lung of rats during development

Cited by 90 publications

References 21 publications

On the Metabolism of Lecithin in Lung and Liver of Fetal Rabbits

On the Metabolism of Lecithin in Lung and Liver of Fetal Rabbits

Mixtures of Low Molecular Weight Surfactant Proteins and Dipalmitoyl Phosphatidylcholine Duplicate Effects of Pulmonary Surfactant in Vitro and in Vivo

Lung Development in the Chick Embryo. I. Phosphatidylcholine Synthesis in the Developing Chick Lung

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