Phospholipid molecular profiles in the seed kernel from different sunflower (Helianthus annuus) mutants

Abstract

Phospholipids are essential components of plant cell membranes whose acyl composition appears to be influenced by oil composition in the sunflower. In the current study, we have determined the diacylglycerol profile of the main phospholipids using phospholipase C degradation and separation of the diacylglycerols by HPLC and GLC. The main polar lipid molecular species were defined in different classes of sunflower kernel: PC, PE, and PI. The proportions of each were determined at different stages of development in order to define the point at which the mutations carried by each sunflower line affected the phospholipid composition of the seeds. The results indicated that modifications to intraplastidial de novo FA synthesis affected the seed phospholipid profile during the whole period of the seed formation, including accumulation and maturation, whereas the influence of mutations in the endoplasmic reticulum desaturases were more readily detected at later stages of development. These results are discussed in terms of the pathways involved in glycerolipid synthesis and phospholipid conversion in sunflower seeds.

Solvent Extraction: Kinetic Study of Major and Minor Compounds

“…On the other hand, phospholipids are the main component of cell membranes and their primary function is to maintain the integrity of the cell membrane under different environmental conditions. It should also be noted that when these compounds are associated with oleosin proteins, they are essential for the construction of oil bodies [21]. The increase in mass of tocopherols and phospholipids over time of extraction is shown in Figs.…”

“…Phospholipids are the main components of cell membranes. Cytosolic, mitochondrial and vacuolar membranes are composed of phospholipids, among which phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol are the most prominent species [21]. Previous evidence indicates that phosphatides are concentrated in the last fraction extracted from soybeans with hexane [22].…”

Effect of Sunflower Collets Moisture on Extraction Yield and Oil Quality

“…Phospholipids are associated with oleosin proteins being essential for the construction of the oil bodies. [28] Moreover, they are the main components of cell membranes being their primary function to maintain the integrity of the cell membrane under different environmental conditions. Thus, an external force is necessary to facilitate their released during oil extraction.…”

Lc-MS and Esi-MS Qtof-MS Analysis of Glycerophospholipid and Triacylglycerol Species in Developing Wild Artichoke Achenes

“…Our hypothesis is in accordance to the biochemical studies of the FA distribution in the glycerolipid molecules of sunflower achenes. 20 The dominating acyl chains combination of PA, PE and PI were respectively C16:0/C18:2, C16:0/C18:1, and C18:0/C18:1 but still be dependent on the stage of seed development. For instance, in the case of PI, 26 DAF were characterized by a higher content of stearoyl-oleoyl (PI-SO) and palmitoyl-linolenoyl PI (PI-PLn) whereas PI-PL and PI-PO were relatively more abundant in fully mature seeds (45 DAF).…”

“…Identification of the phospholipid species and their temporal occurrence provides a possible way to produce seed oil with high additional value. 20 Five major glycerophospholipid classes were determined as phosphatidic acid (PA), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), phosphatidylcholine (PC) and phosphatidylinositol (PI). The focus of the analysis was to monitor the evolution of the individual PL species over maturation however, absolute content could be estimated by peak areas.…”