Phospholipid molecular species distribution of Porphyromonas asaccharolytica ATCC 25260T: effects of temperature, culture age and pH

Tavana, Ali Mehrabi; Drucker, D.B.; Boote, V.

doi:10.1111/j.1365-2672.1998.tb05268.x

Cited by 7 publications

(3 citation statements)

References 18 publications

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“…Experimental growth conditions were strictly controlled because of the effects that age of culture, pH, temperature and nutrients have on cellular lipid composition (9).…”

Section: Discussionmentioning

confidence: 99%

Phospholipid Analogue Profiles of HumanPorphyromonas gingivalisIsolates from Different Geographical Locations

Korachi

Hull

Blinkhorn

et al. 2003

Microbial Ecology in Health and Disease

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Porphyromonas gingivalis displays interesting phospholipid analogue profiles, which can vary between strains from different animal species. The purpose of this study was to compare human strains, isolated in different parts of the world, with respect to their phospholipid profiles. In all, 24 isolates from three continents were cultured and their lipids were extracted with chloroform-methanol and characterized by fast atom bombardment mass spectrometry (FAB-MS) in negative ion mode. Both lower mass (carboxylate) and higher mass (phospholipid analogue) anions were measured in each mass spectrum. Calculation of Pearson correlation coefficients for each strain pair was then followed by average linkage cluster analysis. The major lower mass anion observed for all strains analysed in this study was of mass-to-charge (m/z) 241, which is consistent with previous FAB-MS studies on P. gingivalis. The major phospholipid analogue anion observed for isolates from Manchester, UK, was m/z 960 which is an unknown phospholipid. Other major peaks were of m/z 662 and 663, which may be identified respectively as PE (30:0) and PG (28:1) with contributions from the first isotope of PE (30:0). It was observed that isolates from the same geographical location displayed similar phospholipid profiles. It is concluded that phospholipid profiles of human P. gingivalis isolates are diverse and that there can be differences between P. gingivalis isolates obtained from different geographical locations.

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“…Experimental growth conditions were strictly controlled because of the effects that age of culture, pH, temperature and nutrients have on cellular lipid composition (9).…”

Section: Discussionmentioning

confidence: 99%

Phospholipid Analogue Profiles of HumanPorphyromonas gingivalisIsolates from Different Geographical Locations

Korachi

Hull

Blinkhorn

et al. 2003

Microbial Ecology in Health and Disease

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“…FAB, LC−MS (ESI), and MALDI-TOF mass spectrometry analyses have all been used in the characterization of various microorganisms, − and phospholipid patterns were used to distinguish between different Candida species 15 and for the distinction between C. glabrata and S. cerevisiae . , However, phospholipid distribution analysis using FAB mass spectrometry requires large amounts of cell mass (10−20 mg) in combination with prolonged extraction and analysis methods. Moreover, phospholipid contaminants from cell compartments, such as mitochondria, may also influence the overall result.…”

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confidence: 99%

Application of TOF-SIMS with Chemometrics To Discriminate between Four Different Yeast Strains from the Species Candida glabrata and Saccharomyces cerevisiae

et al. 2005

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We present a TOF-SIMS analysis of the cell surface differences between four yeast strains from two species, Candida glabrata and Saccharomyces cerevisiae (haploid strains BY4742 and BY4741 and the derived diploid BY4743). The study assesses the suitability of TOF-SIMS analysis in combination with statistical methods (principal component analysis, Fisher's discriminant analysis, and cluster analysis) for the discrimination between the four yeast strains. We demonstrate that a combination of these statistical methods identifies 34 ions, from a total data set of 1200, which can be used to distinguish between the four yeasts. The study discusses the assignments of surface cell membrane phospholipids for the identified ions and the resulting differences in the phospholipid pattern between the four yeasts, particularly in relation to ploidy and budding pattern. The method shows that fatty acids, phosphatidylglycerols, phosphatidylethanolamines, phosphatidylserines, and phosphatidylcholines, as well as cardiolipins, are of diagnostic importance.

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“…Lipid extracts were redissolved in methanol (5 µL) then dispersed in equal volumes of m-nitrobenzyl alcohol, the matrix fluid, prior to ionisation by fast atom bombardment with atoms of xenon. Ion separation was performed in negative ion mode as described elsewhere (Tavana et al 1998b).…”

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confidence: 99%

Phospholipid analogue profiles of Peptostreptococcus, Micromonas, and Finegoldia species analysed by fast atom bombardment mass spectrometry

Radcliffe¹,

Drucker²,

Boote³

et al. 2001

Can. J. Microbiol.

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Species of Peptostreptococcus cause a variety of infections, primarily abscesses of soft tissues, joints, and mucous membranes. The aim of this study was to compare the phospholipid analogue profiles of Peptostreptococcus species, represented by P. anaerobius, P. asaccharolyticus, P. indolicus, P. lacrimalis, and P. prevotii; Micromonas micros (P. micros) and Finegoldia magna (P. magnus). After anaerobic growth on blood-FAA, lipids extracted by chloroform-methanol (2:1 v/v) were purified, then analysed by fast atom bombardment mass spectrometry (FAB-MS) in negative ion mode. The major peaks with mass to charge (m/z) 719, 721, and 749, corresponded to phosphatidylglycerol analogues, namely PG (32:1), PG (32:0), and PG (34:0), which have been found previously in Lactobacillus spp., Clostridium difficile, and Staphylococcus spp. Other major peaks observed, with m/z 619, 647, 665, 675, 677, 687, 691, 693, 701, 703, 707, 733, and 746 have also been reported in one or more of these three species. However, other major peaks found here in Peptostreptococcus, Micromonas, and Finegoldia have not been described elsewhere; these are 501, 514, 515, 618, 659, 673, 676, 688, 690, 692, 694, 700, 706, 715, 718, 722, and 750. We conclude that Peptostreptococcus, Micromonas, and Finegoldia isolates are chemically unique.

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Phospholipid molecular species distribution of Porphyromonas asaccharolytica ATCC 25260T: effects of temperature, culture age and pH

Cited by 7 publications

References 18 publications

Phospholipid Analogue Profiles of HumanPorphyromonas gingivalisIsolates from Different Geographical Locations

Phospholipid Analogue Profiles of HumanPorphyromonas gingivalisIsolates from Different Geographical Locations

Application of TOF-SIMS with Chemometrics To Discriminate between Four Different Yeast Strains from the Species Candida glabrata and Saccharomyces cerevisiae

Phospholipid analogue profiles of Peptostreptococcus, Micromonas, and Finegoldia species analysed by fast atom bombardment mass spectrometry

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