Phospholipid monolayers of di-oleoyl lecithin at the mercury/water interface

Nelson, Andrew; Auffret, N.

doi:10.1016/0022-0728(88)80098-6

Cited by 90 publications

(85 citation statements)

References 16 publications

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“…, of solvent-free bilayer lipid membranes 34 and in good agreement with the value of 1.85 mF cm À2 , reported for a mercury-supported DOPC monolayer self-assembled on mercury 35 (for a review, see Ref. 36).…”

Section: à2supporting

confidence: 87%

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

et al. 2010

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Monolayers and bilayers of lipid mixtures self-assembled on mercury form spontaneously gel-phase (solid ordered, s o ) and liquid-ordered (l o ) microdomains, thanks to the fluidity imparted to these films by the liquid metal support. The differential capacity of the hydrocarbon tail region of monolayers of mixtures of two lipid components of high and low transition temperature T m , increases during the transition from the liquid disordered (l d ) phase to the coexistence of l d and s o phases. Addition of cholesterol to this binary mixture causes a decrease in differential capacity. This behavior is explained by regarding the capacity as a measure of the total perimeter of the s o microdomains, due to the mismatch between these microdomains and the l d phase. Cholesterol removes this mismatch by converting the anisotropic s o microdomains into isotropic l o microdomains (rafts). This allows differential capacity measurements by electrochemical impedance spectroscopy to follow phase transitions in lipid mixtures. The coexistance of l d , l o and s o phases is confirmed by images of a distal lipid monolayer self-assembled on top of a thiolipid monolayer tethered to a mercury microcap, by using two-photon fluorescence lifetime imaging microscopy (2P-FLIM).

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Section: à2supporting

confidence: 87%

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

et al. 2010

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“…Monolayers of dioleoyl phosphatidylcholine (DOPC) and DOPC + cholesterol were prepared as described previously (Bizzotto & Nelson, 1998;Cohen-Atiya et al, 2006;Nelson & Auffret, 1988;Whitehouse et al, 2004) by spreading 13 μl of a DOPC + cholesterol mixture in pentane (HPLC grade, Fisher Scientific Chemicals Ltd) at the argon-electrolyte interface in an electrochemical cell. A 2.55 mmol/l working solution of DOPC was obtained by dilution of a 63.7 mmol/l stock solution (Avanti Lipids).…”

Section: Measurement Of Specific Capacitance Of Dopc Membranesmentioning

confidence: 99%

Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

Pásek

Brette

Nelson

et al. 2008

Progress in Biophysics and Molecular Biology

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The transverse (t-) tubules of cardiac ventricular myocytes are invaginations of the surface membrane that form a complex network within the cell. Many of the key proteins involved in excitation-contraction coupling appear to be located predominantly at the t-tubule membrane.Despite their importance, the fraction of cell membrane within the t-tubules remains unclear: measurement of cell capacitance following detubulation suggests ~32%, whereas optical measurements suggest up to ~65%. We have therefore investigated the factors that may account for this discrepancy. Calculation of the combinations of t-tubule radius, length and density that produce t-tubular membrane fractions of 32% or 56% suggest that the true fraction is at the upper end of this range. Assessment of detubulation using confocal and electron microscopy suggests that incomplete detubulation can account for some, but not all of the difference. High cholesterol, and a consequent decrease in specific capacitance, in the t-tubule membrane, may also cause the ttubule fraction calculated from the loss of capacitance following detubulation to be underestimated. Correcting for both of these factors results in an estimate that is still lower than that obtained from optical measurements suggesting either that optical methods overestimate the fraction of membrane in the t-tubules, or that other, unknown, factors, reduce the apparent fraction obtained by detubulation. A biophysically realistic computer model of a rat ventricular myocyte, incorporating a t-tubule network, is used to assess the effect of the altered estimates of t-tubular membrane fraction on the calculated distribution of ion flux pathways.

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“…Being driven by the potential change, some of them clearly show dynamic and reversible structural changes including adsorption/desorption, reorientation, and formation/dissolution of the compact film. The potential-driven structural changes of adsorbed films have been so far demonstrated for dodecylsulfonate [1][2][3][4], octadecanoic acid [1,5,6], 12-(9-anthroyloxy) stearic acid [7], 1-octadecanol [1,6,[8][9][10][11][12][13][14][15]], 4-pentadecyl pyridine (C15-Py) [1,[16][17][18][19][20], phospholipids (dimyristoylphosphatidylcholine and dioleoylphosphatidylcholine) [1,[21][22][23][24][25][26][27][28][29]. Except for the conventional electrochemical methods, used to describe the molecular level details of the structural changes are scanning tunneling microscopy (STM) [2,3,27], atomic force microscopy (AFM) [2], and spectroelectrochemical methods (neutron scattering [4,18,19,28,29], fluorescence …”

Section: Introductionmentioning

confidence: 99%

Voltammetric study of adsorption layers of various 4-pyridyl terminated surfactants on a Au(111) electrode: Effects of electronic property of pyridyl group and intermolecular hydrogen bonding upon potential-driven phase changes

Uematsu

Sagara

2008

Journal of Electroanalytical Chemistry

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The major factors determining the potential-driven phase changes of the films of 4-pyridyl terminated water-insoluble neutral surfactants on a Au(111) electrode were described using the results of dc and ac voltammetric measurements. In total six surfactants were used to elucidate the effects of electronic property of the 4-pyridyl head group, hydrogen bonding ability between amide groups, direction of the amide bond, and bulkiness around the head group upon the potential dependent reorientation and adsorption-desorption processes.Comparison of the behavior of pentadecyl 4-pyridyl ether (C15-O-Py) and 1-pyridin-4-yl-hexadecan-1-one (C15-(C=O)-Py), possessing respectively, the highest and lowest excess electronic charge on the pyridyl nitrogen among the six surfactants, revealed that the latter exhibits more distinct reorientation of pyridyl group corresponding to phase transition between condensed and loose-packed states and narrower potential region of the compact film formation. Introduction of amide group in the alkyl chains of the surfactants reduced the packing of the adsorbed film in the potential region of the compact film formation, as evidenced by an increase of the differential capacitance. It was found that the existence of amide group facilitates the potential-driven phase transition of closely packed film from a condensed state to a loosely packed state, whereas it suppresses the transition of loosely packed film. The concomitant steric effect was also discussed.

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Phospholipid monolayers of di-oleoyl lecithin at the mercury/water interface

Cited by 90 publications

References 16 publications

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

Electrochemical impedance spectroscopy and fluorescence lifetime imaging of lipid mixtures self-assembled on mercury

Quantification of t-tubule area and protein distribution in rat cardiac ventricular myocytes

Voltammetric study of adsorption layers of various 4-pyridyl terminated surfactants on a Au(111) electrode: Effects of electronic property of pyridyl group and intermolecular hydrogen bonding upon potential-driven phase changes

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