Phospholipids. 4. On the composition of hen's egg phospholipids

Rhodes, D. N.; Lea, C. H.

doi:10.1042/bj0650526

Cited by 278 publications

(69 citation statements)

References 18 publications

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“…Subsequently these separated fractions were chromatographed on silicic acid paper, and the percentage of sphingomyelin could then be determined (14). Secondly, aluminum oxide chromatography was used to separate the acidic phospholipids from the choline-containing phospholipids (24). The absorbant aluminum oxide (suitable for chromatography) 3 was washed five times with chloroform: methanol 1:1 (vol/vol) (C: M 1/1) in a beaker, the supernatant containing finely divided A120s being decanted each time.…”

Section: Methodsmentioning

confidence: 99%

Human Fetal Erythrocyte and Plasma Lipids*

Crowley¹,

Ways²,

Jones³

1965

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

Human Fetal Erythrocyte and Plasma Lipids*

Crowley¹,

Ways²,

Jones³

1965

J. Clin. Invest.

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“…Phosphatidylcholine and phosphatidylethanolamine were prepared from egg yolk according to the procedure of Rhodes and Lea [14], and trigljceride from corn oil as described by Hirsch and Ahrens [15]. Protein was determined by the method of Lowry et al [16] with bovine serum albumin as the standard.…”

Section: Reagents Preparations and Determinationsmentioning

confidence: 99%

Acetyl‐Coenzyme‐A Carboxylase in Cultured Hepatocytes

Kitajima

Tashiro

Numa

1975

European Journal of Biochemistry

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Studies were made on the content, synthesis and degradation of acetyl-coenzyme-A carboxylase in JTC-25 . P3 cells, hepatocytes which can be maintained in a protein-free and lipid-free chemically defined medium. The addition of corn oil or fatty acid to the medium resulted in a decrease in the activity level of the enzyme without impairing the viability of cells. All the fatty acids tested exhibited this effect, although linoleic acid and oleic acid were more effective than palmitic acid, stearic acid and arachidonic acid. Immunochemical titration and Ouchterlony double-diffusion analysis indicated that the decrease in the activity level of the enzyme observed in cells incubated in medium supplemented with fatty acid can be ascribed to a reduction of the quantity of the enzyme. Isotopic leucine incorporation studies with the use of immunochemical techniques demonstrated that this reduction of the enzyme content is due to a decrease in the rate of synthesis of the enzyme. The rate of degradation of the enzyme was essentially unaffected, the half-life being 25 and 28 h, respectively, in cells incubated in the presence and absence of fatty acid. It was shown that most of the isotopic fatty acid added to the medium was incorporated into cellular phospholipids, while a very small portion of it was recovered in triglyceride and nonesterified fatty acid.Acetyl-coenzyme-A carboxylase catalyzes the first step leading specifically to long-chain fatty acid synthesis and plays a critical role in the regulation of this synthetic process (see [l]). The activity level of this enzyme in mammalian tissues varies in accord with the rate of fatty acid synthesis under various dietary, hormonal, developmental and genetic conditions of the animal (see [2,3]). Particular emphasis has recently been placed on the questions, whether the variations in the level of acetyl-CoA carboxylase activity in tissue extracts observed under the various conditions are actually determined by changes in the quantity of the enzyme, and if so, whether these changes are due to alterations in the rate of synthesis or in that of degradation of the enzyme. Immunochemical studies with rats subjected to different Enzymes. Acetyl-CoA carboxylase or acetyl-CoA : carbondioxide ligase (ADP-forming) (EC 6.4.1.2) ; lactate dehydrogenase or L-lactate : NAD' oxidoreductase (EC 1.1.1.27); ATP-citrate lyase or ATP : citrate oxaloacetate-lyase (pro-3S-CH2COO-+ acetyl-CoA; ATP-dephosphorylating) (EC 4.1.3.8); malic enzyme or L-malate : NADP' oxidoreductase (oxaloacetate-decarboxylating) (EC 1.1.1.40).dietary manipulations [4-61 and alloxan-diabetes [6] as well as with genetically obese mice [7] demonstrated that the fluctuations of the level of acetyl-CoA carboxylase activity in the liver of these animals reflect changes in the quantity of the enzyme. Furthermore, it was shown with the use of combined immunochemical and isotopic techniques that the increase in the acetyl-CoA carboxylase content of the liver of rats fasted and subsequently refed a fat-free diet can be ascr...

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“…Classical methods for separating and identifying individual phospholipids (Deuel, 1957i Lovern, 1957Witt.-coff, 1951) (Freeman et al, 1957;Hanahan et al, 1957;Philipps, 195#;Rhodes and Lea, 1957) have shown that further fractionation of the phospholipids is possible by elution with chloroformmethanol mixtures. In general, the silicic acid column technique has wide application.…”

Section: Separation and Identification Of Phosphatidesmentioning

confidence: 99%