Phospholipids as implant coatings

Willumeit‐Römer, Regine; Schuster, A.; Iliev, P.; Linser, Sebastian; Feyerabend, Frank

doi:10.1007/s10856-006-0702-9

Cited by 34 publications

(25 citation statements)

References 51 publications

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“…To obtain biologically active materials that provide biological cues for tissue regeneration, various coatings can be applied [8]. One possible approach is a biomimetic coating based on phospholipids that can induce calcification or better cell differentiation [9][10][11][12]. Therefore, biomembranes could serve as a model for the molecular engineering of biocompatible and bioactive surfaces for implantable biomaterials [13].…”

Section: Introductionmentioning

confidence: 99%

X-ray and neutron investigation of self-assembled lipid layers on a titanium surface

et al. 2013

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Titanium is the most widely preferred metal material for bone reconstruction in orthopedics and dentistry. To improve its biological performance, various coatings can be applied. In this investigation, a biomimetic coating on a model implant surface was studied in X-ray and neutron reflectivity experiments to probe the quality of this coating, which is only few nanometers thick. Titanium was deposited on polished silicon surfaces using a magnetron sputtering technique. To improve the lipid coating’s stability, a stronger van der Waals interaction was first created between the implant surface and the biomimetic coating by adding a phosphonic acid (n-octadecylphosphonic acid – OPA) monolayer onto the surfaces. Then, three monolayers of POPE (phospholipid 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-ethanolamine) were transferred using the Langmuir-Blodgett (LB) and Langmuir-Schaefer (LS) techniques. The analysis of X-ray and neutron specular reflectivity data shows that OPA molecules cover the model implant surface completely and that approximately 50% coverage of POPE can be achieved by LB and LS transfer.

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Section: Introductionmentioning

confidence: 99%

X-ray and neutron investigation of self-assembled lipid layers on a titanium surface

et al. 2013

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“…osteoblasts (Zanchetta and Guezennec, 2001). Part of the phospholipid material is exfoliating upon incubation in water or growth medium, but still a significant amount of phospholipid bilayers stays on the titanium alloy substrate (Willumeit et al, 2003b;Willumeit et al, 2007). The mechanical characterisation of these phospholipid layers is very complicated, because none of the typical tests (peeling, fractioning etc.)…”

Section: Discussionmentioning

confidence: 99%

In-vitro interactions of human chondrocytes and mesenchymal stem cells, and of mouse macrophages with phospholipid-covered metallic implant materials

Willumeit¹,

Schossig²,

Clemens³

et al. 2007

eCM

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Phospholipid-coatings on metallic implant surfaces were evaluated in terms of adhesion, proliferation and matrix production of skeletal cells, and of macrophage stimulation. The working hypothesis is that mimicking a model biomembrane by phospholipids on surfaces to which cells adhere, the surface recognition by surrounding cells is altered. In this study, 1) mirror-like polished Ti-6Al-7Nb and 2) porous Ti-6Al-4V specimens were covered with the phospholipids POPE (palmitoyl-oleoyl phosphatidylethanolamine) and POPC (palmitoyl-oleoyl phosphatidylcholine), and the interactions of a) human articular chondrocytes (HAC), b) human mesenchymal stem cells (HMSC), and c) mouse macrophages (RAW 264.7) were tested in vitro.On POPE-covered polished surfaces adherence of HAC (42% of seeded cells after 2 hrs) and metabolic activity (MTT after 3 days) were reduced, while on porous surfaces 99% HAC adhered, and metabolic activity was significantly increased, compared to respective native surfaces. On both POPE-covered surfaces the chondrocyte phenotype was present. After 3 weeks of chondrogenic differentiation, cartilage matrix production (measuring chondroitin sulphate per HAC number) was significantly increased by about 30% on both POPE-covered metallic surfaces. On both POPCcovered surfaces nearly no adhering and surviving HAC were found.HMSC grown on POPE-covered porous substrates showed osteogenic differentiation by improved osteopontin and collagen I expression in RT-PCR, and osteocalcin fluorescence and bone nodule formation was only detectable on POPE-covered porous surfaces. In contrast to POPC and other phospholipids used as positive controls, POPE did not stimulate the NO production in mouse macrophage cultures. We therefore conclude that a phospholipid coating by POPE shows potential as surface modification for metallic implant materials.

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“…Phospholipids are a promising coating material that should mimic natural cell membranes, hide artificial implant material, and strengthen the intimate interface between metallic implant surfaces and bone tissue [26,27].…”

Section: Uts (Ucs) Ys (Cys) εF (εC) (µG/g) (%)mentioning

confidence: 99%

Comparison of the Influence of Phospholipid-Coated Porous Ti-6Al-4V Material on the Osteosarcoma Cell Line Saos-2 and Primary Human Bone Derived Cells

Deing

Ebel

Willumeit‐Römer

et al. 2016

Metals

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Abstract:Biomaterial surface functionalization remains of great interest in the promotion of cell osteogenic induction. Previous studies highlighted the positive effects of porous Ti-6Al-4V and phospholipid coating on osteoblast differentiation and bone remodeling. Therefore, the first objective of this study was to evaluate the potential synergistic effects of material porosity and phospholipid coating. Primary human osteoblasts and Saos-2 cells were cultured on different Ti-6Al-4V specimens (mirror-like polished or porous specimens) and were coated or not with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (POPE) for three weeks or five weeks. Selected gene expressions (e.g., classical bone markers: alkaline phosphatase, osteocalcin, osteoprotegerin (OPG), receptor activator of nuclear factor kappa-β ligand (RANKL) and runt-related transcription factor 2) were estimated in vitro. Furthermore, the expressions of osteocalcin and osteopontin were examined via fluorescent microscopy at five weeks (immunocytochemistry). Consequently, it was observed that phospholipid coating potentiates preferences for low and high porosities in Saos-2 and primary cells, respectively, at the gene and protein levels. Additionally, RANKL and OPG exhibited different gene expression patterns; primary cells showed dramatically increased RANKL expression, whereas OPG expression was decreased in the presence of POPE. A synergistic effect of increased porosity and phospholipid coating was observed in primary osteoblasts in bone remodeling. This study showed the advantage of primary cells over the standard bone cell model.

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Phospholipids as implant coatings

Cited by 34 publications

References 51 publications

X-ray and neutron investigation of self-assembled lipid layers on a titanium surface

X-ray and neutron investigation of self-assembled lipid layers on a titanium surface

In-vitro interactions of human chondrocytes and mesenchymal stem cells, and of mouse macrophages with phospholipid-covered metallic implant materials

Comparison of the Influence of Phospholipid-Coated Porous Ti-6Al-4V Material on the Osteosarcoma Cell Line Saos-2 and Primary Human Bone Derived Cells

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