Phospholipids of Bovine Spermatozoa and Seminal Plasma

Pursel, V. G.; Graham, E.F.

doi:10.1530/jrf.0.0140203

Cited by 53 publications

(35 citation statements)

References 23 publications

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“…The amount of phospholipid extracted from human spermatozoa is similar to the amount extracted from the ram (Scott et al, 1967) and bull (Pursel & Graham, 1967) despite the use of different extraction procedures, but is lower than the figures quoted for the boar (Johnson et al, 1969;Poulos et al, 1972 Stanley (1957) in at least three other species (Poulos et al, 1972).…”

Section: Discussionmentioning

confidence: 52%

“…The phospholipid composition of human spermatozoa appears to resemble that of the boar more closely (Johnson et al, 1969;Poulos et al, 1972) than that of the ram (Scott et al, 1967;Quinn & White, 1967) or bull (Masaki & Hartree, 1962;Pursel & Graham, 1967). Choline plasmalogen comprises only 3% and 10% of human and boar sperm phospholipids, respectively, whereas it is by far the major component of ram and bull spermatozoa.…”

Section: Discussionmentioning

confidence: 91%

“…Sphingomyelin is the predominant phospholipid type present in boar (Johnson et al, 1969) and human seminal plasma but in ram and bull seminal plasma (Quinn & White, 1967;Pursel & Graham, 1967), the choline phospholipids are the major fraction accounting for about half the total phospholipid. The latter phos¬ pholipid fraction comprises only about 10% of the phospholipids of human and boar seminal plasma.…”

Section: Discussionmentioning

confidence: 99%

“…Although a considerable amount of information has been amassed on the phospholipid composition of the spermatozoa of ram (Hartree & Mann, 1961 ;Scott, Voglmayr & Setchell, 1967), bull (Pursel & Graham, 1967) and boar (Grogan, Mayer & Sikes, 1966;Johnson, Gerrits & Young, 1969), little work has been carried out on the phospholipid composition of human spermatozoa. This may be at least partially due to the low sperm concentration of human semen which necessitates the use of large numbers of ejaculates before sufficient lipid material can be isolated and analysed.…”

Section: Introductionmentioning

confidence: 99%

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The Phospholipid Composition of Human Spermatozoa and Seminal Plasma

Poulos¹,

White²

1973

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Summary. The phospholipid composition of human spermatozoa and seminal plasma was examined by quantitative two-dimensional thinlayer chromatography, followed by phosphorus analysis. Sperm phospholipid comprised 28\m=.\8%phosphatidyl choline, 21\m=.\6%phosphatidyl ethanolamine, 21\m=.\4%sphingomyelin, 9\m=.\4% ethanolamine plasmalogen, 4\m=.\7%phosphatidyl serine, 2\m=.\7% choline plasmalogen, 1\m=.\9%phosphatidyl inositol and 1\m=.\6%cardiolipin.Seminal plasma phospholipids comprised 44\m=.\0%sphingomyelin, 12\m=.\3% ethanolamine plasmalogen, 11\m=.\2%phosphatidyl serine, 8\m=.\5% phosphatidyl ethanolamine, 7\m=.\8%phosphatidyl choline, 0\m=.\8% choline plasmalogen, 0\m=.\8% cardiolipin and 1\m=.\7%phosphatidyl inositol.Prolonged aerobic incubation of human spermatozoa in the presence or absence of glucose produced no significant alteration either in the amount of phospholipid phosphorus extracted or in the phospholipid composition of this extracted material.

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Section: Discussionmentioning

confidence: 52%

Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Phospholipid Composition of Human Spermatozoa and Seminal Plasma

Poulos¹,

White²

1973

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“…The fatty acid and fatty aldehyde composition of lipids of spermatozoa and seminal plasma have been examined in the bull, ram, boar, stallion, dog, rabbit, fowl and man (Flipse & Potter, 1955;Scott, White & Annison, 1961 ; Dietz, Pickett, Komarek & Jensen, 1963;Pursel & Graham, 1967; Ahluwalia & Holman, 1969;Johnson, Gerrits & Young, 1969;Poulos, Darin-Bennett & White, 1973; Darin-Bennett, Poulos & White, 1974) and fatty acid composition has usually been given of total semen, total phospholipids or of some component neutral lipid or phospholipid. Precise information about the fatty acid composition of the principal lipid classes and the various compo¬ nent lipids in each class has not been previously available.…”

Section: Introductionmentioning

confidence: 99%

Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid

Jain¹,

Anand²

1976

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Summary. Analysis of the fatty acids of total and neutral lipids, glycolipids, phospholipids and gangliosides of buffalo spermatozoa and seminal plasma showed that there were high levels of polyunsaturated acids. Neutral lipids were the richest in polyunsaturated acids (55 % in spermatozoa and 61 % in seminal plasma). The major saturated acid of all the principal classes was stearic acid and the major unsaturated acid was docosahexaenoic acid (22: 6\g=w\3)except in the neutral lipids in which it was arachidonic acid (20:4\g=w\6).The major aldehyde was palmitaldehyde (16:0) in buffalo sperm lipids and docosanal (22:0) in seminal plasma. More than 50% of the total aldehydes was contributed by aldehydes with a chain length greater than 18 carbon atoms.

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Phosphorylcholine‐binding proteins from the seminal fluids of different species share antigenic determinants with the major proteins of bovine seminal plasma

Leblond

Desnoyers

Manjunath

1993

Molecular Reproduction Devel

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The major proteins of bovine seminal plasma, BSP-A1, BSP-A2, BSP-A3, and BSP-30kDa (collectively named BSP proteins) bind to phospholipids containing the phosphorylcholine moiety. An affinity purification method using a p-aminophenyl phosphorylcholine-Agarose (PPC-Agarose) affinity matrix was developed for their purification. In this study, we investigated the distribution of BSP-like analogues in seminal fluid of the human, porcine, hamster, mouse, and rat using this affinity matrix. Alcohol precipitates of the seminal plasma/seminal vesicle secretions (SP/SVS) were further delipidated using isopropyl ether:n-butanol (60:40). The protein preparations obtained were solubilized in a minimal volume of buffer A (50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 5 mM EDTA, 0.02% NaN3), dialyzed against the same buffer, and applied to a PPC-Agarose column connected to a FPLC system. The unbound material was washed out and the adsorbed proteins eluted with buffer A containing 10 mM phosphorylcholine (PrC) and 10 M urea. The fractions were separated by SDS-PAGE, stained or transferred onto a nitrocellulose membrane, and probed with rabbit polyclonal anti-BSP antibodies. Anti-BSP cross-reacting proteins were detected in the seminal fluids of all the species investigated. Moreover, many of these proteins bound to the affinity matrix. The BSP proteins and their immunoreacting analogues appear to be ubiquitous in mammals and may possibly be involved in a common function such as in the modification of the lipid content of the sperm plasma membrane.

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Phospholipids of Bovine Spermatozoa and Seminal Plasma

Cited by 53 publications

References 23 publications

The Phospholipid Composition of Human Spermatozoa and Seminal Plasma

The Phospholipid Composition of Human Spermatozoa and Seminal Plasma

Fatty acids and fatty aldehydes of buffalo seminal plasma and sperm lipid

Phosphorylcholine‐binding proteins from the seminal fluids of different species share antigenic determinants with the major proteins of bovine seminal plasma

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