Phospholipids of human serum

Abstract

Phospholipids extracted from normal human serum were fractionated into lecithin, lysolecithin, sphingomyelin, phosphatidyl ethanolamine, lysophosphatidyl ethanolamine, phosphatidyl serine, and phosphatidyl inositol. Identification of each was established by thin‐layer chromatography and infrared spectrophotometry. The content of plasmalogen was determined in both lecithin and phosphatidyl ethanolamine fractions. The composition of fatty acids and fatty aldehydes in isolated phospholipids is presented. The degree of unsaturation as reflected in the average content of double bonds per molecule of the fatty acids in phospholipids was: lecithin 1.2, choline plasmalogen 2.1, lysolecithin 0.6, sphingomyelin 0.2, phosphatidyl ethanolamine 2.8, lysophosphatidyl ethanolamine 1.0, phosphatidyl serine 1.0, and phosphatidyl inositol 1.8. Both chlline and ethanolamine plasmalogen aldehydes were predominantly saturated. Molecular weight of each phospholipid was calculated from determined fatty acid and fatty aldehyde compositions; the phosphorus factor for each phospholipid was computed. On a weight percent basis, lecithin, sphingomyelin, and lysolecithin accounted for 95% of the total phospholipids. The ethanolamine‐containing phospholipids accounted for 2.5%, and the remainder was divided among phosphatidyl inositol, choline plasmalogen and phosphatidyl serine.

Studies on Phytoconstituents and Biological Potential of Stem of Moringa oleifera

“…Its Rf value was found to be 0.42 in benzene. Its melting point was found to be 144-146 o C which is in agreement with the literature data 148-150 o C 10 . GC-MS analysis of compound I showed that its molecular mass is 400 with molecular formula C 28 H 48 O.…”

Lipids in Wild Ruminant Animals and Steers

“…Aliquots representing about -75 mg of lipid were used for analysis of free fatty acids (Bergmann et al 1980) and aliquots representing about 20 mg muscle were used for analysis of lipid phosphorus (Dodge and Phillips 1967). Lipid phosphorus values were multiplied by 25 for estimation of phospholipids (Williams et al 1966). Aliquots of lipid solutions representing lipid from about 500 mg muscle were transferred to screw cap (Teflon-lined) culture tubes and aliquots of a known amount of 5-alphacholestane (about 200 micrograms) in hexane were added to each tube as internal standard.…”

LIPIDS IN SUBCUTANEOUS TISSUES AND LONGISSIMUS MUSCLES OF FEEDLOT AND GRASS‐FED EWES1

“…Lipid phosphorus in muscle lipid was determined by procedure of Dodge and Phillips (1967) and these values multiplied by 25 for estimation of phospholipids (Williams et al 1966). After addition of heneicosanoic acid (21:O) as internal standard and evaporation of hexane, portions of subcutaneous and muscle lipids were saponified, nonsaponifiables extracted and discarded, and free fatty acids isolated.…”