Phospholipids of yeast

Letters, R.

doi:10.1016/0005-2760(66)90119-6

Cited by 75 publications

(33 citation statements)

References 29 publications

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“…The phospholipid composition of the mitochondria of wild type diploid yeast (YFp+) is strikingly similar to that reported for mitochondria of several mammalian tissues (Fleischer et al, 1967 ;Getz et al ., 1968) and, more recently, of yeast (Letters, 1966 ;Paltauf and Schatz, 1969) . The phospholipid composition of mitochondria in all of these cell types is characterized by a high proportion of cardiolipin and a higher ratio of phosphatidyl ethanolamine to lecithin than is found in other cellular fractions .…”

Section: Discussionmentioning

confidence: 99%

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

“…This substituted for the overnight room temperature extraction with 19 vol of ethanol (final concentration of ethanol 95%) used with all other yeast samples . This variation was an attempt to limit the phospholipolytic activity in the fractions prior to analysis, as suggested by Letters (1967) . Evidence for the quantitative nature of this extraction procedure has been provided by Letters (1966Letters ( , 1967 and Harrison and Trevelyan (1963) .…”

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

“…This variation was an attempt to limit the phospholipolytic activity in the fractions prior to analysis, as suggested by Letters (1967) . Evidence for the quantitative nature of this extraction procedure has been provided by Letters (1966Letters ( , 1967 and Harrison and Trevelyan (1963) . Although peroxides were not measured directly in the lipid extract, major deterioration of lipids did not ap- $ Abbreviations: DPG, diphosphatidyl glycerol or cardiolipin ; PE, phosphatidyl ethanolamine ; PC, phosphatidyl choline or lecithin ; DMPE, dimethylphosphatidyl ethanolamine, PG, phosphatidyl glycerol ; PA, phosphatidic acid ; PS, phosphatidyl serine ; LPE, lysophosphatidyl ethanolamine ; PI, phosphatidyl inositol ; LPC, lysophosphatidyl choline or lysolecithin ; PLP, phospholipid phosphorus .…”

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

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Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

Jakovcic

Getz

Rabinowitz

et al. 1971

The Journal of Cell Biology

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The phospholipid composition of various strains of the yeast, Saccharomyces cerevisiae, and several of their derived mitochondrial mutants grown under conditions designed to induce variations in the complement of mitochondrial membranes has been examined . Wild type and petite (cytoplasmic respiratory deficient) yeasts were fractionated into various subcellular fractions, which were monitored by electron microscopy and analyzed for cytochrome oxidase (in wild type) and phospholipid composition . 90% or more of the phospholipid, cardiolipin was found in the mitochondrial membranes of wild type and petite yeast . Cardiolipin content differed markedly under various growth conditions . Stationary yeast grown in glucose had better developed mitochondria and more cardiolipin than repressed log phase yeast . Aerobic yeast contained more cardiolipin than anaerobic yeast . Respiration-deficient cytoplasmic mitochondrial mutants, both suppressive and neutral, contained less cardiolipin than corresponding wild types . A chromosomal mutant lacking respiratory function had normal cardiolipin content . Log phase cells grown in galactose and lactate, which do not readily repress the development of mitochondrial membranes, contained as much cardiolipin as stationary phase cells grown in glucose . Cytoplasmic mitochondrial mutants respond to changes in the glucose concentration of the growth medium by variations in their cardiolipin content in the same way as wild type yeast does under similar growth conditions. It is concluded that cardiolipin content of yeast is correlated with, and is a good indicator of, the state of development of mitochondrial membrane .

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Section: Discussionmentioning

confidence: 99%

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

Section: Lipid Extraction and Chromatographic Analysismentioning

confidence: 99%

See 2 more Smart Citations

Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

Jakovcic

Getz

Rabinowitz

et al. 1971

The Journal of Cell Biology

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“…This is due to the challenge to distinguish specific phospholipids in mixed membranes and due to insufficient ssNMR signal sensitivity. These problems could be overcome with 13 C, 15 N-labeled phospholipids that could be selectively detected with high sensitivity in mixed membranes. However, uniformly 13 C, 15 N-labeled phospholipids are not commercially available, and their purification has hitherto not been reported, severely curtailing the scope of ssNMR studies of phospholipids.…”

Section: Introductionmentioning

confidence: 99%

Specific Lipid Studies in Complex Membranes by Solid‐State NMR Spectroscopy

Beekveld

Derks

Kumar

et al. 2022

Chemistry A European J

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Specific interactions with phospholipids are often critical for the function of proteins or drugs, but studying these interactions at high resolution remains difficult, especially in complex membranes that mimic biological conditions. In principle, molecular interactions with phospholipids could be directly probed by solid‐state NMR (ssNMR). However, due to the challenge to detect specific lipids in mixed liposomes and limited spectral sensitivity, ssNMR studies of specific lipids in complex membranes are scarce. Here, by using purified biological 13C,15N‐labeled phospholipids, we show that we can selectively detect traces of specific lipids in complex membranes. In combination with 1H‐detected ssNMR, we show that our approach provides unprecedented high‐resolution insights into the mechanisms of drugs that target specific lipids. This broadly applicable approach opens new opportunities for the molecular characterization of specific lipid interactions with proteins or drugs in complex fluid membranes.

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Sporotrichum Schenkii Ecology Major Lipid Constituents

Arora¹

2022

Preprint

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Phospholipids of yeast

Cited by 75 publications

References 29 publications

Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

Cardiolipin Content of Wild Type and Mutant Yeasts in Relation to Mitochondrial Function and Development

Specific Lipid Studies in Complex Membranes by Solid‐State NMR Spectroscopy

Sporotrichum Schenkii Ecology Major Lipid Constituents

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