Phosphoprotein Profile of Rice (Oryza sativa L.) Seedlings under Osmotic Stress after Pretreatment with Chitosan

Abstract

This study aims to identify novel chitosan (CTS)-responsive phosphoproteins in Leung Pratew 123 (LPT123) and Khao Dawk Mali 105 (KDML105) as drought-sensitive rice cultivars and differences in the CTS response. Rice seeds were soaked in CTS solution before germination, and 2- and 4-week-old rice seedlings sprayed with CTS before osmotic stress comprised the following four groups: (1) seedlings treated with distilled water; (2) seedlings treated with CTS; (3) seedlings pretreated with distilled water and subjected to osmotic stress; and (4) seedlings pretreated with CTS and subjected to osmotic stress. Phosphoproteins of leaf tissues were enriched using immobilized metal affinity chromatography (IMAC) before tryptic digestion and analysis via LC-MS. Phosphoprotein profiling analyses led to the identification of 4721 phosphoproteins representing 1052 and 1040 unique phosphoproteins in the LPT123 and KDML105 seedlings, respectively. In response to CTS pretreatment before osmotic stress, 22 differently expressed proteins were discovered, of which 10 and 12 were identified in the LPT123 and KDML105, respectively. These proteins are typically involved in signaling, transport, protein folding, protein degradation, and metabolism. This study provides fruitful data to understand the signal transduction mechanisms of rice seedlings pretreated with CTS before exposure to osmotic stress.

Phenylalanine Ammonia-Lyase-Mediated Differential Response of Tomato (Solanum lycopersicum L.) Cultivars with Different Stress Tolerance to Treatment with Low-Molecular-Weight Chitosan

“…Browning of the root tip also indirectly indicates the accumulation of phenolic compounds, as mentioned by many researchers [29,39,64,68]. The reduction in plant growth rate upon application of the chitosan is further corroborated by our study on cv.…”

Bioformulations Derived from <i>Enterobacter</i> sp. NRRU-N13 and Oligochitosan Alleviate Drought Stress in Thai Jasmine Rice (<i>Oryza sativa</i> L. var. KDML105)

“…However, the application of the FN13, FCN13, and FON13 bioformulations did not affect proline accumulation in rice plants under normal conditions. Previous studies reported drought-induced proline accumulation in rice leaves ( Xiong et al , 2012 ; Dien et al , 2019 ; Pongprayoon et al , 2022 ).…”

“…Chitosan and its derivatives have been employed as bioactive chemicals or binding materials in mixtures with other substances to promote plant growth and development ( Stasińska-Jakubas and Hawrylak-Nowak, 2022 ) and secure protection against plant pathogens ( Stanley-Raja et al , 2021 ). The involvement of chitosan in plant defense responses to biotic and biotic stresses have been reported in several plants, such as grapes ( Vitis vinifera L.) ( Singh et al , 2019 ) and rice ( O. sativa L.) ( Stanley-Raja et al , 2021 ; Pongprayoon et al , 2022 ).…”

“…They are exploited in agriculture as bioformulations to promote and protect plants from biotic and abiotic stresses by triggering several sensitive genes, proteins, and secondary metabolites ( Limpanavech et al , 2008 ) as well as up-regulating ROS pathway-related genes and enhancing antioxidant defense mechanisms in plants ( Singh et al , 2019 ). Chitosan induces resistance to the osmotic stresses of rice seedlings ( Pongprayoon et al , 2022 ). They stimulate favorable responses in crops, particularly chitosan-sprayed plants, to various abiotic stressors.…”