Phosphoproteomic Analysis in Phaseolus vulgaris Roots Treated with Rhizobium etli Nodulation Factors

Jáuregui-Zúñiga, David; Ortega-Ortega, Yolanda; Pedraza-Escalona, Martha; Reyes‐Grajeda, Juan Pablo; Ruiz, María Isabel Romero; Quinto, Carmen

doi:10.1007/s11105-016-0978-y

Cited by 2 publications

(3 citation statements)

References 41 publications

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“…Meanwhile, the expression of PvAnn1 in P. vulgaris was slightly upregulated in developing nodules (CarrascoCastilla et al, 2018). How ever, a phosphoproteomic approach revealed that PvAnn1 was a phosphorylated protein with enhanced levels of synthesis during nodulation (JáureguiZúñiga et al, 2016). Hence, the regulation of annexins involved in nodulation might be dif ferent and is probably connected with different functions that annexins fulfil in this process.…”

Section: Discussionmentioning

confidence: 99%

“…Another annexin, MtAnn3 (Medtr4g097180), was found to be important for root hair deformations in M. truncatula (Gong et al, 2012). At the same time, close homologs of MtAnn1 -PvAnn1 (Phvul.011g209300) and LjAnn1 (Lj0g3v0203419), which belong to the same phylogenetic group as MtAnn1, play important roles in the symbiotic process in P. vulgaris and L. japonicus (Wienkoop, Saalbach, 2003;JáureguiZúñiga et al, 2016;CarrascoCastilla et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

“…A protein homologous to MtAnn1 -PvAnn1 from Phaseo lus vulgaris -is activated at the early stages of symbiosis development (JáureguiZúñiga et al, 2016;CarrascoCastilla et al, 2018). The stimulation of Ca 2+ ion transfer through the plasma membrane and ROS production caused by Nod factors constitute an early response in the signal transduction pathway.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

et al. 2021

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Annexins as Ca2+/phospholipid-binding proteins are involved in the control of many biological processes essential for plant growth and development. In a previous study, we had shown, using a proteomic approach, that the synthesis of two annexins is induced in pea roots in response to rhizobial inoculation. In this study, phylogenetic analysis identified these annexins as PsAnn4 and PsAnn8 based on their homology with annexins from other legumes. The modeling approach allowed us to estimate the structural features of these annexins that might influence their functional activity. To verify the functions of these annexins, we performed comparative proteomic analysis, experiments with calcium influx inhibitors, and localization of labeled proteins. Essential down-regulation of PsAnn4 synthesis in a non-nodulating pea mutant P56 (sym10) suggests an involvement of this annexin in the rhizobial symbiosis. Quantitative RT-PCR analysis showed that PsAnn4 was upregulated at the early stages of symbiosis development, starting from 1–3 days after inoculation to up to 5 days after inoculation, while experiments with the Ca2+ channel blocker LaCl3 revealed its negative influence on this expression. To follow the PsAnn4 protein localization in plant cells, it was fused to the fluorophores such as red fluorescent protein (RFP) and yellow fluorescent protein (YFP) and expressed under the transcriptional regulation of the 35S promoter in Nicotiana benthamiana leaves by infiltration with Agrobacterium tumefaciens. The localization of PsAnn4 in the cell wall or plasma membrane of plant cells may indicate its participation in membrane modification or ion transport. Our results suggest that PsAnn4 may play an important role during the early stages of pea-rhizobial symbiosis development.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

et al. 2021

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The seed-borne Southern bean mosaic virus hinders the early events of nodulation and growth in Rhizobium-inoculated Phaseolus vulgaris L.

López

Muñoz

Lascano

et al. 2017

Functional Plant Biol.

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To simulate seed-borne virus transmission, a noninvasive protocol was designed to infect the radicle of germinating seeds, with 100% effectiveness. Preinfection of 24-h-old black bean (Phaseolus vulgaris L.) radicles by Southern bean mosaic virus (SBMV) followed by Rhizobium inoculation 48 h later caused a drastic reduction in root nodulation. Results were attributed to active virus replication within the elongating zone of the radicle at least 32 h before Rhizobium inoculation, which elicited severe anatomical malformations; an abnormal accumulation of apoplastic reactive oxygen species in the rhizodermis, cortex, inner cortical and endodermic root cells; the formation of atypical root hair tips and the collapse of 94% of the root hairs in the SBMV-preinfected radicles. Adult SBMV-preinfected plants showed exacerbated virus symptoms and 80% growth reduction ascribed to major virus-induced ultrastructural alterations in the nodules. The accumulation of ureides, α−amino acids and total reducing sugars in the leaves and nodules of SBMV-preinfected plants are indicators of the hindering effects of SBMV infection on N2 fixation and ureide catabolism, causing N starvation. The exogenous addition of 1 or 4 μM naringenin, genistein or daidzein did not counteract the deleterious effects of SBMV preinfection on nodulation.

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Phosphoproteomic Analysis in Phaseolus vulgaris Roots Treated with Rhizobium etli Nodulation Factors

Cited by 2 publications

References 41 publications

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

Phylogenetic and structural analysis of annexins in pea (<i>Pisum sativum</i> L.) and their role in legume-rhizobial symbiosis development

The seed-borne Southern bean mosaic virus hinders the early events of nodulation and growth in Rhizobium-inoculated Phaseolus vulgaris L.

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