Phosphoproteomic analysis of lettuce (Lactuca sativa L.) reveals starch and sucrose metabolism functions during bolting induced by high temperature

Abstract

High temperatures induce early bolting in lettuce (Lactuca sativa L.), which decreases both quality and production. However, knowledge of the molecular mechanism underlying high temperature promotes premature bolting is lacking. In this study, we compared lettuce during the bolting period induced by high temperatures (33/25 °C, day/night) to which raised under controlled temperatures (20/13 °C, day/night) using iTRAQ-based phosphoproteomic analysis. A total of 3,814 phosphorylation sites located on 1,766 phosphopeptides from 987 phosphoproteins were identified after high-temperature treatment,among which 217 phosphoproteins significantly changed their expression abundance (116 upregulated and 101 downregulated). Most phosphoproteins for which the abundance was altered were associated with the metabolic process, with the main molecular functions were catalytic activity and transporter activity. Regarding the functional pathway, starch and sucrose metabolism was the mainly enriched signaling pathways. Hence, high temperature influenced phosphoprotein activity, especially that associated with starch and sucrose metabolism. We suspected that the lettuce shorten its growth cycle and reduce vegetative growth owing to changes in the contents of starch and soluble sugar after high temperature stress, which then led to early bolting/flowering. These findings improve our understanding of the regulatory molecular mechanisms involved in lettuce bolting.

Bio-Based Solutions for Agriculture: Foliar Application of Wood Distillate Alone and in Combination with Other Plant-Derived Corroborants Results in Different Effects on Lettuce (Lactuca Sativa L.)

“…Studies on the effect of WD on starch are scanty: Sun-Ok and Dong-Hoon [43] found that WD concentrations of up to 0.5% decreased the leaf starch content of the orchid Neofinetia falcata but increased its root content. We have not assayed the root parts of the plant, but the starch content of our control lettuce leaves was consistent with that reported in other studies [44].…”

Shifts of the soil microbiome composition induced by plant–plant interactions under increasing cover crop densities and diversities

“…Incidentally, the abundance of the antiprotozoal microbe Oscillatoria nigro-viridis increased between brassica densities of 24 and 48 plants 39 . It was previously found that intraspecific competition in B. juncea manifested as increased counts of stress induced inflorescences and bolting 40 , 41 . In the present study, brassica bolted at the 24 and 48 plant densities which could be attributed to increasing intraspecific competition.…”

A Genome-Wide Analysis of the BAM Gene Family and Identification of the Cold-Responsive Genes in Pomegranate (Punica granatum L.)

“…The starch and sucrose metabolism pathway plays a pivotal role in plant abiotic stress responses, including salt [34,35], drought [36], high temperature [37], and cold [38][39][40]. This pathway contributes to the accumulation of soluble sugars, thereby increasing cellular permeation concentration and water potential, and ultimately enhancing stress tolerance.…”