Phosphoproteomics analysis reveals the anti-bacterial and anti-virulence mechanism of eugenol against Staphylococcus aureus and its application in meat products

Li, Hong; Li, Changzhu; Shi, Ce; Alharbi, Metab; Cui, Haiying; Lin, Lin

doi:10.1016/j.ijfoodmicro.2024.110621

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…The results of this study showed that the F. retinophylla extract inhibited the motility process of S. aureus which could be related to the bioactive compounds present in the extract. For example, it was previously shown that phenolic compounds (luteolin, -hydroxyemodin and 3-hydroxybenzoic acid) and terpenes (eugenol and (+)-nootkatone) affect the quorum sensing system of S. aureus and its agr system [ 38 , 39 , 40 , 41 , 42 ], inhibiting the transcriptional units RNAII and RNAIII, which are involved in the production of virulence factors such as phenol-soluble modulin [ 38 , 43 , 44 ]. Furthermore, the agr quorum sensing system of S. aureus is associated with the bacterial membrane [ 45 ].…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial and Antibiofilm Potential of Flourensia retinophylla against Staphylococcus aureus

Beltrán-Martínez,

Tapia-Rodríguez,

Ayala-Zavala

et al. 2024

Plants

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Staphylococcus aureus is a Gram-positive bacteria with the greatest impact in the clinical area, due to the high rate of infections and deaths reaching every year. A previous scenario is associated with the bacteria’s ability to develop resistance against conventional antibiotic therapies as well as biofilm formation. The above situation exhibits the necessity to reach new effective strategies against this pathogen. Flourensia retinophylla is a medicinal plant commonly used for bacterial infections treatments and has demonstrated antimicrobial effect, although its effect against S. aureus and bacterial biofilms has not been investigated. The purpose of this work was to analyze the antimicrobial and antibiofilm potential of F. retinophylla against S. aureus. The antimicrobial effect was determined using an ethanolic extract of F. retinophylla. The surface charge of the bacterial membrane, the K+ leakage and the effect on motility were determined. The ability to prevent and remove bacterial biofilms was analyzed in terms of bacterial biomass, metabolic activity and viability. The results showed that F. retinophylla presents inhibitory (MIC: 250 µg/mL) and bactericidal (MBC: 500 µg/mL) activity against S. aureus. The MIC extract increased the bacterial surface charge by 1.4 times and the K+ concentration in the extracellular medium by 60%. The MIC extract inhibited the motility process by 100%, 61% and 40% after 24, 48 and 72 h, respectively. The MIC extract prevented the formation of biofilms by more than 80% in terms of biomass production and metabolic activity. An extract at 10 × MIC reduced the metabolic activity by 82% and the viability by ≈50% in preformed biofilms. The results suggest that F. retinophylla affects S. areus membrane and the process of biofilm formation and removal. This effect could set a precedent to use this plant as alternative for antimicrobial and disinfectant therapies to control infections caused by this pathogen. In addition, this shrub could be considered for carrying out a purification process in order to identify the compounds responsible for the antimicrobial and antibiofilm effect.

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