Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation

Abstract

Mahonia bealei (M. bealei) is a traditional Chinese medicine containing a high alkaloid content used to treat various diseases. Generally, only dried root and stem are used as medicines, considering that the alkaloid content in M. bealei leaves is lower than in the stems and roots. Some previous research found that alkaloid and flavonoid contents in the M. bealei leaves may increase when exposed to ultraviolet B (UV-B) radiation. However, the underlying mechanism of action is still unclear. In this study, we used titanium dioxide material enrichment and mass-based label-free quantitative proteomics techniques to explore the effect and mechanism of M. bealei leaves when exposed to UV-B treatment. Our data suggest that UV-B radiation increases the ATP content, photosynthetic pigment content, and some enzymatic/nonenzymatic indicators in the leaves of M. bealei. Moreover, phosphoproteomics suggests phosphoproteins related to mitogen-activated protein kinase (MAPK) signal transduction and the plant hormone brassinosteroid signaling pathway as well as phosphoproteins related to photosynthesis, glycolysis, the tricarboxylic acid cycle, and the amino acid synthesis/metabolism pathway are all affected by UV-B radiation. These results suggest that the UV-B radiation activates the oxidative stress response, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway, which may lead to the accumulation of secondary metabolites in M. bealei leaves.

An analysis of natural variation inPinus pinasterthrough the lens of systems biology

“…These proteins had a direct correlation with the three features (Figure 8B). The abundance of a second malic enzyme (MAE), also unraveled as relevant by PCA and MOFA (Figure 4 and 6), correlated positively with the abundance of columbamine (Figure 8B), a secondary metabolite increasing under UV-B exposure (Liu et al, 2022).…”

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“…RNA extraction and qRT-PCR analysis were performed according to Liu et al [ 46 ]. The primer information used in this study is shown in Table S1 .…”

Integrative Proteomic and Phosphoproteomic Analyses Revealed the Regulatory Mechanism of the Response to Ultraviolet B Stress in Clematis terniflora DC

“…Moreover, UV-B stress also has the potential to increase the secondary metabolite contents, which can be used by plants for stress resistance . Berberine, jateorhizine, palmatine, and columbamine were increased when Mahonia bealei was exposed to UV-B radiation combined with dark treatment . It has been reported that the concentration of kaempferol increases after UV-B radiation in Arabidopsis thaliana .…”

“… 8 Berberine, jateorhizine, palmatine, and columbamine were increased when Mahonia bealei was exposed to UV-B radiation combined with dark treatment. 9 It has been reported that the concentration of kaempferol increases after UV-B radiation in Arabidopsis thaliana . ( 10 ) UV-B followed by dark treatment also increased the contents of coumarins in C. terniflora according to a previous study, 11 and the ATP content increased to provide energy for secondary metabolism.…”