Phosphorescent iridium(iii) complexes capable of imaging and distinguishing between exogenous and endogenous analytes in living cells

Abstract

Phosphorescent iridium(iii) complexes bearing two carbon chains are able to distinguish between endogenous and exogenous analytes when serving as luminescent cellular probes.

Time-resolved analysis of photoluminescence at a single wavelength for ratiometric and multiplex biosensing and bioimaging

“…In another experiment, HeLa cells were pretreated with elesclomol (125 nM, 37 °C, 2 h) to induce production of endogenous hypochlorite. 51 The cells were then incubated with P1 (200 μg mL −1 , 37 °C, 1 h). The PLIM image showed substantial short-lived signals <300 ns ( Fig.…”

One‐Step Fabrication of Perovskite‐Based Upconversion Devices

“…In recent years, wavelength interconversion has proven to be a promising technique in many optoelectronic applications. In particular, photon upconversion (UC) creates higher energy light from lower energy light and has applications in in vivo biological imaging and other biomedical applications, [1][2][3] photocatalysis, [4][5][6][7][8] sub-bandgap sensitization of photovoltaics to surpass the Shockley-Queisser [9,10] limit or low-cost nightvision devices. [11] There are several distinct routes possible for creating higher energy photons, including non-linear frequency generation, UC based on the ladder-like energy levels in lanthanide ions [12][13][14] and triplet-triplet annihilation (TTA) in organic semiconductors.…”

Cell‐Membrane Staining Properties and Photocytotoxicity of a Ruthenium(II) Photosensitizer

“…The positively‐charged ruthenium(II) polypyridine core allows the electronic attraction of the complex with negatively‐charged cell surface, while the two lipophilic carbon chains facilitate the hydrophobic‐hydrophobic interactions with phospholipid in the cell membrane. [ 20–23 ] Complex 1 was synthesized via a reported procedure [ 20 ] and characterized via 1 H and 13 C nuclear magnetic resonance (NMR) spectroscopy (Figure S1) and matrix‐assisted laser desorption ionization time‐of‐flight (MALDI‐TOF) mass spectrometry (MS) (Figure S2). Complex 1 displayed a typical singlet metal‐to‐ligand charge‐transfer ( 1 MLCT) absorption band centered at 457 nm in methanol at room temperature (Figure 1).…”