-Phosphorescent Mesoporous Surface Imprinting Microspheres: Preparation and Application for Transferrin Recognition from Biological Fluids

Abstract

The relationship between the thickness of surface molecularly imprinted polymers (MIPs) and specific recognition performance of transferrin (Trf) as well as the quantitative relation between the grafting amount of Mn-ZnS room-temperature phosphorescence (RTP) quantum dots (QDs) (short for PQDs) and RTP signals for recognition of Trf was analyzed in this study. Based on analysis results, RTP protein mesoporous imprinting microspheres (SiO 2 −PQDs−MIPs) with high specificity and strong interference resistance were developed using a mesoporous SiO 2 nanomaterial that can create more three-dimensional precise recognition sites as the matrix and using PQDs with strong resistance to background fluorescence interference as the luminescent materials. A discriminatory analysis of Trf was realized by the phosphorescence quenching principle based on light quenching caused by the photoinduced electron transfer. The concentration range, limit of detection, relative standard deviation, and imprinting factor of Trf detection under pH 7.4 are 0.05−1.0 μM, 0.014 μM, 3.23%, and 3.09, respectively. Although the sensing signals of SiO 2 −PQDs−MIPs for proteins are based on the phosphorescence of PQDs, they are particularly suitable for specific recognition and accurate quantitative detection of proteins in biological fluids. Research conclusions are expected to realize high-efficiency recognition of target proteins in actual biological samples.

A Review on the Application of Zeolites and Mesoporous Silica Materials in the Removal of Non-Steroidal Anti-Inflammatory Drugs and Antibiotics from Water

“…They are used to remove pharmaceuticals from water, which is confirmed by the results presented below. It is also possible to modify their properties through different processes (grafting and co-condensation) [ 111 , 112 , 113 ] or by creating hybrid core/sheath structures, which allows new applications such as molecular printing [ 114 , 115 ].…”

Designing and controlling the morphology of spherical molecularly imprinted polymers

“…Therefore, the solid core-mesoporous shell MIPs are widely applied for construction of chemical sensors or target enrichment. Generally Fe 3 O 4 217,218 and SiO 2 [219][220][221][222][223][224][225] are employed as the solid core and the mesoporous shell layer is formed during the molecular imprinting process. The preparation process is similar to that of core-shell MIPs.…”

Fabrication of Surface-Imprinted Magnetic Wrinkled Microspheres and Their Specific Adsorption of BSA

“…Protein imprinting is different from small-molecule imprinting, and the difficulty in mass transfer is a core problem. − Therefore, surface protein imprinting has become an effective method and research focus. − Strictly speaking, a material with the thickness of the imprinting layer less than or equal to the maximum dimension of protein molecules is called a surface-imprinting material. For example, for a bovine serum albumin (BSA) molecule, the molecular size is 14 × 4 × 4 nm, so the imprinting layer thickness of the surface-imprinting material should be less than 14 nm.…”