Phosphorimetric investigation of several tryptophan metabolites: Determination of Kynurenic Acid in Urine

Effects of external heavy atoms and other factors on the room-temperature phosphorescence and fluorescence of tryptophan and tyrosine

“…aminations of the fluorescence characteristics of the aromatic amino acids and their peptides have been reported (3)(4)(5)(6)(7)(8)(9). The usefulness of phosphorescence in identifying tryptophan and other indole derivatives has been recognized for some time (10,11), and tyrosine phosphorescence has also proved important in some protein studies (12). Interest in the triplet states of these amino acids is further increased by the observations of triplet-singlet energy transfer in proteins from tryptophan donors (13) and of phosphorescence from tryptophan residues of enzymes in room-temperature solutions (14).…”

“…In the external heavy-atom effect, environmental atoms of high atomic numbers enhance molecular spin-forbidden transitions via a spin-orbital coupling mechanism (20). This effect has been employed to enhance phosphorescence intensity in both low-temperature (11,21,22) and room-temperature (18) studies of tryptophan and related compounds.…”

Development of luminescence spectrometry as an analytical tool

“…After 1965, the field of analytical phosphorimetry was developed vigorously and almost exclusively by the Winefordner group. A large number of papers were published on the phosphorescence characteristics of various types of drugs and related compounds including sulfonamides (108,109), anticoagulants (110), tryptophan metabolites (111), antimetabolites (112), vitamins (113,114), plant growth hormones (115), purines (116), catecholamine metabolites (117), hallucinogens (118), and carcinogens (119).…”

Room Temperature Phosphorimetry as a New Spectrochemical Method of Analysis

“…Taylor and Francis shall not be liable for any losses, actions, claims, proceedings, demands, costs, expenses, damages, and other liabilities whatsoever or howsoever caused arising directly or indirectly in connection with, in relation to or arising out of the use of the Content. pharmaceutical compounds in biological fluids [18][19][20][21][22][23][24][25][26] . Only recently has phosphorimetry been extensively developed into a practical qualitative and quantitative analytical method.…”