Phosphorus-31 relaxation times of 2,3-diphosphoglycerate in intact human erythrocytes

31P NMR relaxation time studies of 2,3‐diphosphoglycerate in solution and intact erythrocytes

“…Contrary to the work of Lubansky et al (7) the result of our study show that there are not significant influences on 31P Tl values of 2,3-DPG upon oxygenation or deoxygenation in erythrocytes. Furthermore, Lubansky et al (7) have reported a pronounced difference in the behavior of the T , values of the two phosphorus nuclei in 2,3-DPG upon deoxygenation. We do not find such a difference.…”

“…The T I values in Table 4 represent the most accurate determination in all our measurements on erythrocytes. In agreement with Lubansky et al (7) we find that the Tl values of erythrocytes vary between different collections of blood samples. Furthermore, the time of measurements must be kept very low (less than f h) in order to obtain accurate TI values since the erythrocytes gradually lose energy with a decreasing concentration of 2,3-DPG as a consequence.…”

“…The T, values of 2,3-DPG in erythrocytes given in Table 4 can be compared with the results given by Lubansky et al (7). From the results in Fig.…”

“…Lubansky et al (7) have used TI relaxation measurement of 31P nuclei in 2,3-diphosphoglycerate (2, to study the binding of the molecule to oxy and deoxy hemoglobin in intact human erythrocytes. This same type of binding in a model solution of 2,3-DPG and hemoglobin in water has been studied by Gupta et al (8) using this technique.…”

31P-MRS of healthy human brain: revealing the hidden PME signals under phosphoethanolamine and phosphocholine resonances at 7T

“…24,26,31 Over the last three decades, a few attempts have been made to include 2,3-DPG in PME fitting, [32][33][34] though they differ widely on the details of lineshape model, due to a lack of obvious spectral features assignable to 2,3-DPG. In this study, the strategy to selectively detect h-PME was based on the T1 shortening of 2,3-DPG in RBC, 35,36 rather than on the classic resonance pattern of 2,3-DPG, considering that the resonance frequencies of 2,3-DPG in RBC may be dispersed due to sensitivity to variations in pH, oxygenation, and magnetic anisotropic susceptibility effect from spatial orientation of blood vessels. 24,31,[35][36][37][38] The in vivo brain spectral features documented in this study clearly demonstrated complexity of PME composition and inadequacy of the conventional two-component model.…”