Phosphorus distribution in three crustacean zooplankton species

Vrede, Tobias; Andersen, Tom; Hessen, Dag O.

doi:10.4319/lo.1999.44.1.0225

Cited by 86 publications

(87 citation statements)

References 21 publications

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“…One possible explanation is the relatively small contribution of exoskeleton P to the total P content of daphnids. Although the exoskeleton became P-richer with the elevation of Ca content, this effect is overshadowed by the changes in P content in the somatic tissues and eggs which contain more than 80% of the total P of daphnids (Vrede et al 1999). The major pool of P in Daphnia galeata is the nucleic acids, which account for 35 to 60% of the total P content, followed by the phospholipids which con- We therefore speculate that the higher P content of daphnids in the lower Ca environment was due to the higher demand of nucleic acids and phospholipids involved in the active regulation of Ca efflux.…”

Section: Discussionmentioning

confidence: 99%

“…Although the exoskeleton became P-richer with the elevation of Ca content, this effect is overshadowed by the changes in P content in the somatic tissues and eggs which contain more than 80% of the total P of daphnids (Vrede et al 1999). The major pool of P in Daphnia galeata is the nucleic acids, which account for 35 to 60% of the total P content, followed by the phospholipids which con- tribute 20 to 25% to the total P (Vrede et al 1999). Therefore, the observed effects of Ca on the P content of daphnids should primarily be exerted on the nucleic acids and phospholipids.…”

Section: Discussionmentioning

confidence: 99%

“…The efflux rate constant (k e ) and loss budget of P in age 7 d Daphnia magna were also quantified at the 4 Ca levels. Newly born D. magna (< 24 h) were collected and cultured in M7 medium for 7 d before the efflux experiment, and the daphnids were labeled with 33 P by feeding them with 33 P-labeled algae during the last 4 d of this period (long enough to achieve homogeneous labeling; Vrede et al 1999). The radioactive algae were prepared by culturing Chlamydomonas reinhardtii in 200 ml WC medium (initial cell density 2 × 10 5 cells ml -1 ) spiked with 5.18 × 10 3 kBq of 33 P under continuous light for 2 to 5 d. In each treatment, 180 D. magna individuals were cultured in 1.6 l water.…”

Section: Methodsmentioning

confidence: 99%

“…Most Ca in Daphnia is bound within the carapace and ~90% of Ca in Daphnia magna is lost during 1 molting event (Alstad et al 1999). Although the major pool of P in D. magna and D. galeata is nucleic acids and phospholipids contained in the soft tissues, Vrede et al (1999) found that more than 14% of P is distributed within the carapace, possibly bound with Ca. Sterner et al (1993) found that D. obtusa on a P-deficient diet has problems in molting.…”

Section: Introductionmentioning

confidence: 99%

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Calcium influence on phosphorus regulation in Daphnia magna: implications for phosphorus cycling

Tan¹,

Wang

2009

Aquat. Biol.

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We examined the influence of environmental calcium on phosphorus biokinetics and regulation in Daphnia magna. When the Ca concentration in culturing media increased from 0.5 to 200 mg l -1 , the specific P content of D. magna feeding on P-sufficient algae decreased significantly from 1.43 to 1.05% of dry weight. There was a significant negative relationship between the specific Ca and P contents (% dry wt) of daphnids. However, measured biokinetic parameters, including the assimilation efficiency (AE) of dietary P, the weight-specific ingestion rate (IR), the uptake of inorganic P from water, and the efflux rate constant (k e ) of P, were similar over the wide range of Ca concentrations, with some exceptions at the lowest Ca level (0.5 mg l -1 ). Diet was the dominant source of P for daphnids, and only 1 to 2% of P was incorporated from water, based on the biokinetic calculation. With the elevation of the Ca level from 0.5 to 200 mg l -1 , the proportion of P lost through molting by D. magna feeding on P-sufficient diets increased 2.5-fold, whereas the dissolved release decreased 1.9-fold. P released into the dissolved phase is readily re-utilized by primary producers, while P contained within the shed molts sinks out of the epilimnion and becomes at least temporarily unavailable to primary producers; thus, the effects of Ca on molting and dissolved release have significant implications for P cycling in freshwater lakes. Our results suggest that the availability of P to primary producers can be reduced by sedimentation of P-rich daphnid molts in high-Ca lakes, and Ca concentration in the water needs to be considered in studying the stoichiometric regulation of P in freshwater cladocerans. KEY WORDS: Phosphorus regulation · Phosphorus cycling · Phosphorus biokinetics · Effects of calcium · Daphnia magna Resale or republication not permitted without written consent of the publisherThe relative importance of different routes of phosphorus loss from Daphnia magna (via excretion, reproduction and molting) is affected by the calcium level in the water. Photo: Ke Pan OPEN PEN ACCESS CCESSAquat Biol 5: [1][2][3][4][5][6][7][8][9][10][11] 2009 of P is distributed within the carapace, possibly bound with Ca. Sterner et al. (1993) found that D. obtusa on a P-deficient diet has problems in molting. Shedding molts accounts for ~70 and ~50% of P loss from juvenile and adult D. magna over a 3 to 4 d period, respectively (He & Wang 2007). The link between Ca and P in D. magna is also indicated by the crystalline calcium phosphate composition of the shell of its ephippia (Kawasaki et al. 2004). Ca levels in the water considerably affect the calcification of the carapace of daphnids (Alstad et al. 1999) and, thus, their total Ca content (Jeziorski & Yan 2006, Tan & Wang 2008. It is therefore tempting to ask whether the content and distribution of P in daphnids is also affected by the aqueous Ca level, considering the close association of these 2 elements in these organisms. Among the various freshwater zooplankton s...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Calcium influence on phosphorus regulation in Daphnia magna: implications for phosphorus cycling

Tan¹,

Wang

2009

Aquat. Biol.

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“…Indeed, strong couplings between l, RNA and P have been documented in various daphnids (DeMott et al, 1998;Vrede, Andersen & Hessen, 1999;Acharya, Kyle & Elser, 2004), the bacterium Escherichia coli (Makino et al, 2003), cyanobacteria (Lepp & Schmidt, 1998), yeast (Aiking & Tempest, 1976), algae (Rhee, 1978) and other biota . While there are consistent general patterns within species and groups of species, considerable variation in the relationships of RNA and P contents with growth does exist among taxa.…”

Section: Introductionmentioning

confidence: 99%

Coupling of growth rate and body stoichiometry in Daphnia: a role for maintenance processes?

et al. 2006

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1. The growth rate hypothesis predicts positive relationships among growth rate (l), body RNA (%RNA of dry mass) and body P (%P of dry mass) contents. 2. We tested this within-and across-species by growing five species/clones of Daphnia (Daphnia magna, Daphnia pulex, Daphnia galeata and two isolates of Daphnia pulicaria) with different combinations of food quantity and stoichiometric food quality. 3. Within each species, positive correlations among l, %RNA and %P were seen and across species there was a strong association between%RNA and %P, consistent with the growth rate hypothesis. However, coupling of growth to %RNA and to %P differed for different species. In particular, the %RNA-l and %P-l relationships had similar slopes but considerably different y-intercepts (i.e.%P or %RNA at zero growth), with D. pulicaria and D. galeata having higher intercepts than D. magna and especially D. pulex. As a result of these displacements, the relative rankings of the species on the basis of %P and %RNA did not correspond to their rankings based on l. 4. These findings suggest that within a narrow clade (e.g. the daphnids), interspecific differences in body P content may reflect not growth rate-related RNA allocation but instead the amount of RNA required for support of maintenance processes.

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An experimental test of the growth rate hypothesis as a predictive framework for microevolutionary adaptation

Lemmen

Zhou

Papakostas

et al. 2022

Ecology

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The growth rate hypothesis (GRH) posits that the relative body phosphorus content of an organism is positively related to somatic growth rate, as protein synthesis, which is necessary for growth, requires P-rich rRNA. This hypothesis has strong support at the interspecific level. Here, we explore the use of the GRH to predict microevolutionary responses in consumer body stoichiometry.For this, we subjected populations of the rotifer Brachionus calyciflorus to selection for fast population growth rate (PGR) in P-rich (HPF) and P-poor (LPF) food environments. With common garden transplant experiments, we demonstrate that in HP populations evolution toward increased PGR was concomitant with an increase in relative phosphorus content. In contrast, LP populations evolved higher PGR without an increase in relative phosphorus content. We conclude that the GRH has the potential to predict microevolutionary change, but that its application is contingent on the environmental context. Our results highlight the potential of cryptic evolution in determining the performance response of populations to elemental limitation of their food resources.

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Phosphorus distribution in three crustacean zooplankton species

Cited by 86 publications

References 21 publications

Calcium influence on phosphorus regulation in Daphnia magna: implications for phosphorus cycling

Calcium influence on phosphorus regulation in Daphnia magna: implications for phosphorus cycling

Coupling of growth rate and body stoichiometry in Daphnia: a role for maintenance processes?

An experimental test of the growth rate hypothesis as a predictive framework for microevolutionary adaptation

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