Phosphorus nuclear magnetic resonance spectroscopy: <i>In vivo</i> magnesium measurements in the skeletal muscle of normal subjects

Ward, Kathleen; Rajan, Sunder S.; Wysong, Melissa; D, Radulović; Clauw, Daniel J.

doi:10.1002/mrm.1910360323

Cited by 8 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, we considered the significantly lower [Mg 2+ ] as observed in the whole DMD data set, which was investigated in this study only with 31 P NMRS, as a genuine finding, with decreased [Mg 2+ ] between 10% and 18% in the anterior and posterior compartment of the leg, respectively, compared with normal values ranging between 14% and 20% in the anterior part of the forearm of ambulant and nonambulant patients, respectively. The normal [Mg 2+ ] values as derived in our study were approximately 0.53 ± 0.05mM and correspond well with values in the literature for skeletal muscle of healthy male children (0.51 ± 0.03mM) 25 , and between 0.52 and 0.56mM in two other studies performed in normal adult subjects 23,28 . Iotti et al, however, found lower muscle Mg 2+ concentrations of~0.30mM in controls, which was due to them taking into account additional Mg 2+ formation constants (corresponding to exchange processes with other Mg 2+ binding ligands) 26 .…”

Section: [Mg 2+ ] In Extended 31 P Nmrs Databasesupporting

confidence: 91%

“…Free intramuscular magnesium concentration ([Mg 2+ ]) can be determined with phosphorus nuclear magnetic resonance spectroscopy ( 31 P NMRS), based on the chemical shift between the αATP and βATP resonances or between phosphocreatine (PCr) and βATP. The assessment of [Mg 2+ ] is highly dependent on a precise knowledge of intracellular pH because of a competition between H + and Mg 2+ ions for binding with ATP, with ion changes having an effect on its chemical shift.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

2019

View full text Add to dashboard Cite

Early studies have demonstrated that (total) magnesium was decreased in skeletal muscle of Duchenne muscular dystrophy (DMD) patients. Free intramuscular Mg2+ can be derived from 31P NMRS measurements. The value of free intramuscular magnesium concentration ([Mg2+]) is highly dependent on precise knowledge of intracellular pH, which is abnormally alkaline in dystrophic muscle, possibly due to an expanded interstitial space, potentially causing an underestimation of [Mg2+]. We have recently shown that intracellular pH can be derived using 1H NMRS of carnosine. Our aim was to determine whether 31P NMRS‐based [Mg2+] is, in fact, abnormally low in DMD patients, taking advantage of the 1H NMRS‐based pH. A comparative analysis was, therefore, made between [Mg2+] values calculated with both 1H and 31P NMRS‐based approaches to determine pH in 25 DMD patients, on a 3‐T clinical NMR scanner. [Mg2+] was also assessed with 31P NMRS only in (forearm or leg) skeletal muscle of 60 DMD patients and 63 age‐matched controls. Additionally, phosphodiester levels as well as quantitative NMRI indices including water T2, fat fraction, contractile cross‐sectional area and one‐year changes were evaluated. The main finding was that the significant difference in [Mg2+] between DMD patients and controls was preserved even when the intracellular pH determined with 1H NMRS was similar in both groups. Consequently, we observed that [Mg2+] is significantly lower in DMD patients compared with controls in the larger database where only 31P NMRS data were obtained. Significant yet weak correlations existed between [Mg2+] and PDE, water T2 and fat fraction. We concluded that low [Mg2+] is an actual finding in DMD, whether intracellular pH is normal or alkaline, and that it is a likely consequence of membrane leakiness. The response of Mg2+ to therapeutic treatment remains to be investigated in neuromuscular disorders. Free [Mg2+] determination with 31P NMRS is highly dependent on a precise knowledge of intracellular pH. The pH of Duchenne muscular dystrophy (DMD) patients, as determined by 31P NMRS, is abnormally alkaline. We have recently shown that intracellular pH could be determined using 1H NMRS of carnosine, and that intracellular pH was alkaline in a proportion of, but not all, DMD patients with a 31P NMRS‐based alkaline pH. Taking advantage of this 1H NMRS‐based intracellular pH, we found that free intramuscular [Mg2+] is in fact abnormally low in DMD patients.

show abstract

Section: [Mg 2+ ] In Extended 31 P Nmrs Databasesupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

2019

View full text Add to dashboard Cite

show abstract

“…An analogous titration curve can be found for the resonances of ATP, which depend on the concentration of Mg ++ according to the equilibrium: Since the interaction with Mg shifts the ATP resonances (γ > β > α), this titration allows for an estimation of the magnesium concentration in skeletal muscle (61, 133–137).…”

Section: Structural Aspects Of Skeletal Muscle That Influence Mr Spectramentioning

confidence: 91%

Musculoskeletal spectroscopy

Boesch

2007

Magnetic Resonance Imaging

113

View full text Add to dashboard Cite

Magnetic resonance spectroscopy (MRS) of skeletal muscle has been successfully applied by physiologists over several decades, particularly for studies of high-energy phosphates (by (31)P-MRS) and glycogen (by (13)C-MRS). Unfortunately, the observation of these heteronuclei requires equipment that is typically not available on clinical MR scanners, such as broadband capability and a second channel for decoupling and nuclear Overhauser enhancement (NOE). On the other hand, (1)H-MR spectra of skeletal muscle can be acquired on many routine MR systems and also provide a wealth of physiological information. In particular, studies of intramyocellular lipids (IMCL) attract physiologists and endocrinologists because IMCL levels are related to insulin resistance and thus can lead to a better understanding of major health problems in industrial countries. The combination of (1)H-, (13)C-, and (31)P-MRS gives access to the major long- and short-term energy sources of skeletal muscle. This review summarizes the technical aspects and unique MR-methodological features of the different nuclei. It reviews clinical studies that employed MRS of one or more nuclei, or combinations of MRS with other MR modalities. It also illustrates that MR spectra contain additional physiological information that is not yet used in routine clinical applications.

show abstract

“…Since the advent of larger bore spectrometers, human studies in healthy individuals have been carried out showing the usefulness of in vivo 31 P MRS to study normal muscle [23][24][25][26][27][28][29][30][31][32][33]4,[35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53], liver [54,55], kidney [56], brain [4,7,[57][58][59][60][61][62], testes [63], spleen [64], skin [65,66], heart [67,68], breast [69], uterus [70], and blood…”

Section: In Vivo Markers Of Disease Using 31 P Mrsmentioning

confidence: 99%

In Vivo Measurement of Phosphorous Markers of Disease

Arias‐Mendoza

Brown²

2004

Disease Markers

View full text Add to dashboard Cite

Phosphorus Magnetic Resonance Spectroscopy (31P-MRS) has been utilized to study energy, carbohydrate, and phospholipid metabolism in vitro and in vivo in live tissues non-invasively. Despite its lack of sensitivity, its application has extended to in situ human tissues and organs since proper signal localization was devised. Follow-up of phosphocreatine in neuromuscular diseases and schizophrenia and follow-up of phospholipid-related molecules in tumors are described here to demonstrate the value of 31P-MRS as an imaging technique to determine in vivo markers of disease and in the diagnosis, prognosis, and follow-up of human diseases.

show abstract

Phosphorus nuclear magnetic resonance spectroscopy: In vivo magnesium measurements in the skeletal muscle of normal subjects

Abstract: 31P nuclear magnetic resonance spectroscopy was used to study the skeletal muscle of 33 normal males and 32 females. Free intracellular magnesium levels and the ratios of the phosphorus metabolites were determined. Males had significantly lower free magnesium levels (499.8 pM f 26.3 pM vs.

Cited by 8 publications

References 23 publications

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

Musculoskeletal spectroscopy

In Vivo Measurement of Phosphorous Markers of Disease

Contact Info

Product

Resources

About

Phosphorus nuclear magnetic resonance spectroscopy: In vivo magnesium measurements in the skeletal muscle of normal subjects

Cited by 8 publications

References 23 publications

Free intramuscular Mg2+ concentration calculated using both 31P and 1H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

Free intramuscular Mg2+ concentration calculated using both 31P and 1H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

Musculoskeletal spectroscopy

In Vivo Measurement of Phosphorous Markers of Disease

Contact Info

Product

Resources

About

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients

Free intramuscular Mg²⁺ concentration calculated using both ³¹P and ¹H NMRS‐based pH in the skeletal muscle of Duchenne muscular dystrophy patients