Phosphorus Nutrition of Proteaceae in Severely Phosphorus-Impoverished Soils: Are There Lessons To Be Learned for Future Crops?

Lambers, Hans; Finnegan, Patrick M.; Laliberté, Étienne; Pearse, Stuart J.; Ryan, Megan H.; Shane, Michael W.; Veneklaas, Erik J.

doi:10.1104/pp.111.174318

Cited by 188 publications

(141 citation statements)

References 97 publications

(101 reference statements)

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“…Consistent with previous studies (Shane et al, 2003;UhdeStone et al, 2003;Lambers et al, 2011;Wang et al, 2014): (1) 6-week-old, Pi-deprived white lupin plants formed abundant CRs (>50 % of total root biomass, data not shown) with a developmental sequence of juvenile, mature, senescing and senesced CRs visible along the lateral root axis (Fig. 1); (2) a significant increase in PEPC specific activity occurred during CR development that peaked at maturity (4-6 d) then decreased during senescence ($10 d) (Fig.…”

Section: Cluster Root Pepc Of Illuminated White Lupin Plants Is Phospsupporting

confidence: 82%

“…The upregulation of PEPC during Pi starvation has also been linked to the synthesis and exudation of large quantities of organic acid anions (e.g. malate, citrate) by roots of various ÀPi plants (Neumann et al, 2000;Vance et al, 2003;Shane and Lambers, 2005;Plaxton and Tran, 2011;Cheng et al, 2011;Lambers et al, 2011). This greatly increases root Pi acquisition by solubilizing otherwise inaccessible sources of mineralized soil Pi, thus increasing soluble Pi concentrations by up to 1000-fold (Vance et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

“…This greatly increases root Pi acquisition by solubilizing otherwise inaccessible sources of mineralized soil Pi, thus increasing soluble Pi concentrations by up to 1000-fold (Vance et al, 2003). This adaptive strategy is especially prevalent in highly specialized cluster (proteoid) roots (CRs), which mediate efficient Pi acquisition by certain plant species acclimating to soluble Pi-impoverished soils (Keerthisinghe et al, 1998;Neumann et al, 2000;Watt and Evans, 1999;Shane and Lambers, 2005;Cheng et al, 2011;Lambers et al, 2011). Of the various plants that form CRs, white lupin (Lupinus albus) stands out as an important agricultural crop, and is the species that has been most intensively investigated with respect to Pi nutrition (Cheng et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Shane

Feil

Lunn

et al. 2016

Ann Bot

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Background and Aims Phosphoenolpyruvate carboxylase (PEPC) is a tightly regulated enzyme that controls carbohydrate partitioning to organic acid anions (malate, citrate) excreted in copious amounts by cluster roots of inorganic phosphate (Pi)-deprived white lupin plants. Excreted malate and citrate solubilize otherwise inaccessible sources of mineralized soil Pi for plant uptake. The aim of this study was to test the hypotheses that (1) PEPC is post-translationally activated by reversible phosphorylation in cluster roots of illuminated white lupin plants, and (2) light-dependent phosphorylation of cluster root PEPC is associated with elevated intracellular levels of sucrose and its signalling metabolite, trehalose-6-phosphate.Methods White lupin plants were cultivated hydroponically at low Pi levels ( 1 mM) and subjected to various light/ dark pretreatments. Cluster root PEPC activity and in vivo phosphorylation status were analysed to assess the enzyme's diurnal, post-translational control in response to light and dark. Levels of various metabolites, including sucrose and trehalose-6-phosphate, were also quantified in cluster root extracts using enzymatic and spectrometric methods.Key Results During the daytime the cluster root PEPC was activated by phosphorylation at its conserved Nterminal seryl residue. Darkness triggered a progressive reduction in PEPC phosphorylation to undetectable levels, and this was correlated with 75-80 % decreases in concentrations of sucrose and trehalose-6-phosphate.Conclusions Reversible, light-dependent regulatory PEPC phosphorylation occurs in cluster roots of Pi-deprived white lupin plants. This likely facilitates the well-documented light-and sucrose-dependent exudation of Pi-solubilizing organic acid anions by the cluster roots. PEPC's in vivo phosphorylation status appears to be modulated by sucrose translocated from CO 2 -fixing leaves into the non-photosynthetic cluster roots.

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Section: Cluster Root Pepc Of Illuminated White Lupin Plants Is Phospsupporting

confidence: 82%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Shane

Feil

Lunn

et al. 2016

Ann Bot

Self Cite

View full text Add to dashboard Cite

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“…In addition, due to the scarce mobility of Pi, plant uptake, via root epidermal cells and root hairs, leads to a rapid exhaustion of Pi availability near the root system, and creates a depleted zone . In order to increase Pi uptake, plants have evolved different strategies, such as the enhanced growth of lateral roots and root hairs, and/or the solubilization of soil Pi by means of organic acid and phosphatase secretion: all these processes are probably orchestrated by a systemic signalling that is triggered during Pi starvation (Doener 2008;Lambers et al 2011;Péret et al 2011) and involves specific gene expression regulators, as has been demonstrated in Arabidopsis (Pérez Torres et al 2009). Another widespread and evolutionary ancient strategy is the establishment of arbuscular mycorrhizal (AM) symbiosis which involves the majority of land plants and fungi belonging to the Glomeromycota phylum (Parniske 2008;Bonfante and Genre 2010).…”

Section: Introductionmentioning

confidence: 99%

The expression of GintPT, the phosphate transporter of Rhizophagus irregularis, depends on the symbiotic status and phosphate availability

Fiorilli

Lanfranco

Bonfante

2013

Planta

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The development of mutualistic interactions with arbuscular mycorrhizal (AM) fungi is one of the most important adaptation of terrestrial plants to face mineral nutrition requirements. As an essential plant nutrient, phosphorus uptake is acknowledged as a major benefit of the AM symbiosis, but the molecular mechanisms of its transport as inorganic phosphate (Pi) from the soil to root cells via AM fungi remain poorly known. Here we monitored the expression profile of the high-affinity phosphate transporter (PT) gene (GintPT) of Rhizophagus irregularis (DAOM 197198) in fungal structures (spores, extraradical mycelium and arbuscules), under different Pi availability, and in respect to plant connection. GintPT resulted constitutively expressed along the major steps of the fungal life cycle and the connection with the host plant was crucial to warrant GintPT high expression levels in the extraradical mycelium. The influence of Pi availability on gene expression of the fungal GintPT and the Medicago truncatula symbiosis-specific Pi transporter (MtPT4) was examined by qRT-PCR assay on microdissected arbusculated cells. The expression profiles of both genes revealed that these transporters are sensitive to changing Pi conditions: we observed that MtPT4 mRNA abundance is higher at 320 than at 32 μM suggesting that the flow towards the plant requires high concentrations. Taken on the whole, the findings highlight novel traits for the functioning of the GintPT gene and offer a molecular scenario to the models describing nutrient transfers as a cooperation between the mycorrhizal partners.

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“…Indeed, another type of root, 'cluster roots', comprising a morphology of dense clusters of rootlets from a parent root, can be observed in some species, most notably members of the Proteaceae family. These cluster roots are formed as part of the normal root developmental programme rather than as a responsive change [105], and are an example of a root structure which appears to have evolved primarily for the maximization of phosphorus acquisition from the soil [106,107]. In this issue, Nussaume et al [108] discuss a novel imaging technique for the visualization of nutrient uptake.…”

Section: Control Of Root Branching In Arabidopsis (A) Hormonesmentioning

confidence: 99%

Root system architecture: insights fromArabidopsisand cereal crops

Smith

Smet

2012

Phil. Trans. R. Soc. B

383

289

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Roots are important to plants for a wide variety of processes, including nutrient and water uptake, anchoring and mechanical support, storage functions, and as the major interface between the plant and various biotic and abiotic factors in the soil environment. Understanding the development and architecture of roots holds potential for the exploitation and manipulation of root characteristics to both increase food plant yield and optimize agricultural land use. This theme issue highlights the importance of investigating specific aspects of root architecture in both the model plant Arabidopsis thaliana and (cereal) crops, presents novel insights into elements that are currently hardly addressed and provides new tools and technologies to study various aspects of root system architecture. This introduction gives a broad overview of the importance of the root system and provides a snapshot of the molecular control mechanisms associated with root branching and responses to the environment in A. thaliana and cereal crops.

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Phosphorus Nutrition of Proteaceae in Severely Phosphorus-Impoverished Soils: Are There Lessons To Be Learned for Future Crops?

Cited by 188 publications

References 97 publications

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

Light-dependent activation of phosphoenolpyruvate carboxylase by reversible phosphorylation in cluster roots of white lupin plants: diurnal control in response to photosynthate supply

The expression of GintPT, the phosphate transporter of Rhizophagus irregularis, depends on the symbiotic status and phosphate availability

Root system architecture: insights fromArabidopsisand cereal crops

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