Phosphorus Starvation Response in Plants and Opportunities for Crop Improvement

Pandey, Bharati; Mehra, Poonam; Giri, Jitender

doi:10.1002/9783527675265.ch37

Cited by 10 publications

(10 citation statements)

References 133 publications

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“…Under P deficiency we found a significant decrease in primary root length of chickpea which corresponds to the Arabidopsis phenotype (Pandey et al, 2013 ). P deficiency inhibits the primary root growth, increases lateral root length and enhances root hair length and density (Lynch, 2011 ; Niu et al, 2012 ).…”

Section: Discussionsupporting

confidence: 52%

JAZ Repressors: Potential Involvement in Nutrients Deficiency Response in Rice and Chickpea

et al. 2015

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Jasmonates (JA) are well-known phytohormones which play important roles in plant development and defense against pathogens. Jasmonate ZIM domain (JAZ) proteins are plant-specific proteins and act as transcriptional repressors of JA-responsive genes. JA regulates both biotic and abiotic stress responses in plants; however, its role in nutrient deficiency responses is very elusive. Although, JA is well-known for root growth inhibition, little is known about behavior of JAZ genes in response to nutrient deficiencies, under which root architectural alteration is an important adaptation. Using protein sequence homology and a conserved-domains approach, here we identify 10 novel JAZ genes from the recently sequenced Chickpea genome, which is one of the most nutrient efficient crops. Both rice and chickpea JAZ genes express in tissue- and stimuli-specific manners. Many of which are preferentially expressed in root. Our analysis further showed differential expression of JAZ genes under macro (NPK) and micronutrients (Zn, Fe) deficiency in rice and chickpea roots. While both rice and chickpea JAZ genes showed a certain level of specificity toward type of nutrient deficiency, generally majority of them showed induction under K deficiency. Generally, JAZ genes showed an induction at early stages of stress and expression declined at later stages of macro-nutrient deficiency. Our results suggest that JAZ genes might play a role in early nutrient deficiency response both in monocot and dicot roots, and information generated here can be further used for understanding the possible roles of JA in root architectural alterations for nutrient deficiency adaptations.

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Section: Discussionsupporting

confidence: 52%

JAZ Repressors: Potential Involvement in Nutrients Deficiency Response in Rice and Chickpea

et al. 2015

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“…Traditional and modern genotypes differ significantly in their RSA under P deficiency. Higher density of root hairs, increased lateral root length and density contribute significantly to improve the P deficiency by enhancing P uptake from soil solution to plant cells 53 . We identified 7 missense variants in PSR genes involved in RSA modulation like lateral root development (Table S10).…”

Section: Resultsmentioning

confidence: 99%

Genome-wide DNA polymorphisms in low Phosphate tolerant and sensitive rice genotypes

Mehra

Pandey

Giri

2015

Sci Rep

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Soil Phosphorus (P) deficiency is one of the major challenges to rice crop world-wide. Modern rice genotypes are highly P-responsive and rely on high input of P fertilizers. However, low P tolerant traditional cultivars and landraces have genetic potential to sustain well under low P. Identification of high resolution DNA polymorphisms (SNPs and InDels) in such contrasting genotypes is largely missing for low P response at gene levels. Here, we report high quality DNA polymorphisms in low P sensitive genotype, PB1 and tolerant traditional genotype, Dular. We performed whole genome resequencing using Illumina NGS platform and identified a total of 5,157,939 sequence variants in PB1 and Dular with reference to Nipponbare genome. We have identified approximately 2.3 million and 2.9 million high quality polymorphisms in PB1 and Dular, respectively, with an average read depth of ≥24X. We further mapped several DNA polymorphisms (non-synonymous and regulatory variants) having potential functional significance to key Phosphate Starvation Responsive (PSR) and root architecture genes in Dular and Kasalath using a compiled list of low P responsive genes. These identified variants can serve as a useful source of genetic variability for improving low P tolerance and root architecture of high yielding modern genotypes.

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“…Thus, upregulation of OsGDPDs after 7 days of different nutrient deficiencies might play a role in regulating PA homeostasis within cell for controlling root growth or root hair formation. Modulation of root system architecture plays essential roles in nutrient acquisition (López-Bucio et al 2003;Pandey et al 2013). Further, some studies have reported differential regulation of GDPDs under -K and -Fe in rice (Ma et al 2012;Zheng et al 2009).…”

Section: Discussionmentioning

confidence: 96%

Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme

Mehra

Giri

2016

Plant Cell Rep

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Rice and chickpea GDPD s are transcriptionally influenced by mineral deficiencies; especially, by phosphate starvation and CaGDP1 encodes an active glycerophosphodiester phosphodiesterase enzyme. Glycerophosphodiester phosphodiesterases (GDPDs) are enzymes involved in the degradation of glycerophosphodiesters into sn-glycerol-3-phosphate and corresponding alcohols. These phospholipid remodeling genes have been suggested to play important roles in phosphate homeostasis. However, comprehensive information about the role of GDPDs under low phosphate (P) and other nutrient deficiencies (N, K, Fe, Zn) in rice and chickpea is missing. Here, we identified 13 OsGDPDs and 6 CaGDPDs in rice and chickpea, respectively, and partly characterized their roles in multiple nutrient stresses. Expression profiling after 7 and 15 days of deficiency treatments revealed unique and overlapping differential expression patterns of OsGDPDs and CaGDPDs under different nutrient stresses. Principal component analysis on the expression patterns of OsGDPDs and CaGDPDs revealed their preferential role in P starvation. Some of the GDPDs were also induced by N, K, Fe and Zn deficiency in temporal manner in both crops suggesting their roles in multiple nutrient stresses. Biochemical characterization of highly responsive chickpea GDPD, CaGDPD1, confirmed its in vitro GDPD activity and revealed its optimal temperature, pH and cofactor requirements. Further, CaGDPD1 showed its accumulation in ER and endomembranes. We hereby propose CaGDPD1 and various OsGDPDs as low P responsive marker genes in chickpea and rice, respectively. Our data uphold role of GDPDs in multinutrient responses and suggest them as candidates for rice and chickpea improvement for tolerance to various nutrient deficiencies.

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Phosphorus Starvation Response in Plants and Opportunities for Crop Improvement

Cited by 10 publications

References 133 publications

JAZ Repressors: Potential Involvement in Nutrients Deficiency Response in Rice and Chickpea

JAZ Repressors: Potential Involvement in Nutrients Deficiency Response in Rice and Chickpea

Genome-wide DNA polymorphisms in low Phosphate tolerant and sensitive rice genotypes

Rice and chickpea GDPDs are preferentially influenced by low phosphate and CaGDPD1 encodes an active glycerophosphodiester phosphodiesterase enzyme

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