Phosphorus strategy in bloom-forming cyanobacteria (Dolichospermum and Microcystis) and its role in their succession

Wan, Lingling; Chen, Xiaoyan; Deng, Qinghui; Yang, Liuyan; Li, Xiaowen; Zhang, Junyi; Song, Changchun; Zhou, Yiyong; Cao, Xiuyun

doi:10.1016/j.hal.2019.02.007

Cited by 86 publications

(42 citation statements)

References 59 publications

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“…During the transition from Dolichospermum to Microcystis in Lake Taihu and Chaohu, the competitive advantage of Microcystis spp. was displayed at low P concentrations: where it could rapidly uptake and store inorganic P, which also increased the P de ciency of the coexisting phytoplankton species [35]. Also, in this study, compared to bacteria attached Dolichospermum, bacteria attached to Microcystis displayed signi cantly higher abundances of functional genes (phosphatase genes) mediating organic P hydrolysis ( Fig.…”

Section: Discussionmentioning

confidence: 53%

“…Also, the coarse GLU, with a signi cantly higher percentage and activities in Ponds A and B where Microcystis was absolutely dominant throughout the experiment than in Ponds C and D (Fig. 8), should be attributed to bacteria attached to Microcystis, now that Microcystis has no ability to produce extracellular enzymes [35]. All these results indicated that Microcystis secreted a large amount of extracellular organic C surrounding the cell surface, which fuelled the DNRA.…”

Section: For N and C Cyclesmentioning

confidence: 86%

“…The P-solubilizing bacteria (PSB) attached to Microcystis showed lower numbers, higher P-solubilizing ability and more extensive substrate adaptability than that of attached to Dolichospermum [36]. Furthermore, it has been proven that Microcystis has no ability to produce extracellular alkaline phosphatase to hydrolyse organic P [35]. So, the advantage of P acquisition of Microcystis and organic P hydrolysis by bacteria attached to Microcystis mutually drove Microcystis growth and succession from Dolichospermum to Microcystis [28,36].…”

Section: Discussionmentioning

confidence: 99%

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Community composition and functional genes as well as ecological role of bacteria attached to different bloom-forming cyanobacteria

Song¹,

Liu²,

Cao³

et al. 2020

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Background Eutrophication leads to frequent outbreaks of cyanobacterial blooms, especially those of the genera Dolichospermum and Microcystis. The contribution of bacteria attached to algal cells to cyanobacterial blooms is still not clear and specific. To gain a deeper understanding of functional genes and their role in bacteria attached to different bloom-forming cyanobacteria, we carried out microbial experiments associated with Dolichospermum and Microcystis in four fish ponds. Results The significantly positive relationships between Dolichospermum density and total nitrogen (TN) and between Microcystis density and particle nitrogen (PN) indicated the strong nitrogen (N) demand of these two species. The lack of functional genes mediating the nitrification process in bacteria attached to both Microcystis and Dolichospermum indicated that these two species preferred ammonium (NH 4 + -N). Dolichospermum could overcome N limitation through N 2 fixation expressed by high nitrogenase genes abundance. Compared to bacteria attached to Dolichospermum cells, bacteria attached to Microcystis cells showed a higher activity of leucine aminopeptidase (LAP) and a significantly higher abundance of functional genes (such as nrfA , nirB and aminopeptidase genes) mediating the dissimilatory nitrate reduction to ammonium (DNRA). The significantly higher abundance of functional genes (carbon degradation) and β-glucosidase (GLU) activity of bacteria attached to Microcystis than those of bacteria attached to Dolichospermum suggested the abundant organic carbon bound Microcystis cells, which was prerequisite for DNRA. Also, Microcystis had a great advantage in solving phosphorus (P) stress, including high levels of organic phosphorus hydrolysis associated with high levels of phosphatase genes of attached bacteria. The difference of functional community compositions of bacteria attached to Microcystis and Dolichospermum resulted in the functional differentiation. Conclusions Dolichospermum and Microcystis growth was susceptible to P and N (especially NH 4 + -N) limitation, respectively, the latter of which could be effectively solved by attached bacteria through DNRA and ammonification. The P acquisition disadvantage of Dolichospermum resulted in its frequent replacement by Microcystis , especially in conditions of P deficiency. Hence, the evaluation of nutrient limitation (N or P) type of algal growth should combine the nutrient concentration and ratio as well as the ability to solve nutrient deficiency.

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Section: Discussionmentioning

confidence: 53%

Section: For N and C Cyclesmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 99%

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Community composition and functional genes as well as ecological role of bacteria attached to different bloom-forming cyanobacteria

Song¹,

Liu²,

Cao³

et al. 2020

Preprint

Self Cite

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“…This hypothesis was verified by the field observation in Lake Chaohu that high percentage of ELF labeling was accompanied by high biomass. Our previous work about another N 2 -fixing genus Dolichospermum flos-aquae also suggested that it suffered from severe P limitation at the end of the bloom [24]. An obviously lower cell P quota was observed in cyanobacteria during their bloom (unpublished data) and its underlying mechanism needs further investigation.…”

Section: Discussionmentioning

confidence: 87%

“…150 μL of Tris-HCl (pH = 8.5) was added to 2.7 mL subsample, after pipetting aliquots of fluorogenic substrate (MUFP-phosphate, final concentration of 100 μM), fluorescence was measured immediately, after incubation at room temperature for 2 h, fluorescence was read again. The contribution of APA to the algal (APA >3.0μm ) and bacterial (APA 0.45-3.0μm ) fractions were calculated as follows: APA >3.0μm = APA T − APA <3.0μm , APA 0.45-3.0μm = APA <3.0μm − APA <0.45μm , in which APA <0.45μm represented the dissolved phosphatase activity [24].…”

Section: Size-fractionation Of Apamentioning

confidence: 99%

Strategies adopted by Aphanizomenon flos-aquae in response to phosphorus deficiency and their role on growth

et al. 2020

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Background: The N 2 -fixing cyanobacterium, Aphanizomenon flos-aquae is a globally distributed bloom causing species that degrades water quality of fresh and marine water bodies. Overcoming phosphorus (P) deficiency is one of the ecological advantages for bloom-forming cyanobacteria. It remains unclear to what extent can A. flos-aquae alleviate P deficiency by regulating P using strategies.Results: Based on in situ observations of extracellular alkaline phosphatase (APase) in A. flos-aquae via enzymelabeled fluorescence in freshwater bodies in China, Poland and Czechia, we further investigated responses of isolated A. flos-aquae to different P supplies (dissolved inorganic P (Pi) as +DIP, dissolved organic α-glycerophosphate and β-glycerophosphate as +DOP α and +DOP β , P-free condition as P-depleted). The significantly negative relationships between percentage of APase producing cells and soluble reactive P concentration in both fields and cultures suggested that the excretion of APase in cyanobacterium was regulated by ambient Pi supply. Suffering from P deficiency in the P-depleted treatments, A. flos-aquae showed the highest APase activity but a vigorous growth at the early culture stage, which might also benefit from the formation of polyphosphate body (PPB) and the decrease of cell P quota. In the +DOP treatments, the coordination of dissolved DOP mineralization and continuous prompt utilization of PPB might contribute to a maintenance but not reproduction of A. flos-aquae when relying on DOP, since the specific growth rate kept around 0 cells L −1 day −1 at the second half culture period and the highest cell density reached only 13.38% of that in +DIP treatments while photochemical efficiency was comparable during the whole experiment. Luxury uptake of phosphate as PPB in the +DIP treatments was consistent to the canonical view of polyphosphate as P storage. Conclusion:Aphanizomenon flos-aquae could achieve an instantaneous growth in response to P deficiency with the coordination of P utilization strategies, while it maintained a long-term sustainable growth but not reproduction under sole DOP supply. Persistent and active reproduction could only be achieved in high Pi supply, which implying that an effective consequence can be expected for combating the bloom of A. flos-aquae when controlling P supply.

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Watershed, lake, and food web factors influence diazotrophic cyanobacteria in mountain lakes

Jansen,

Sobota,

Pan

et al. 2024

Limnology & Oceanography

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Cyanobacterial blooms can occur in freshwater ecosystems largely isolated from development and not experiencing extensive cultural eutrophication. For example, remote mountain lakes can experience intense blooms of diazotrophic (nitrogen‐fixing) cyanobacteria caused by factors acting at different spatial and temporal scales. In this study, we examined how cross‐scale interactions among watershed, lake, and food web characteristics influence diazotrophic cyanobacteria biovolume in mountain lakes. We quantified diazotrophic cyanobacteria biovolume, zooplankton abundance, and physico‐chemical variables for 29 lakes in the Cascade Mountains of Oregon, USA, in summer 2019. Watershed characteristics were compiled from historical datasets available for the region. Diazotrophic cyanobacteria biovolume ranged across the lakes from 0 to 1,930,000 μm3 mL−1; Dolichospermum was the most common genus. Random forest models showed that 11 watershed, lake, and food web characteristics explained 76% of the variance in diazotrophic cyanobacteria biovolume among the sampled lakes. Structural equation models suggested that the drainage ratio (i.e., the relative area of the lake to the watershed) was positively related to phosphorus concentrations and, in turn, to diazotroph biovolume. Among lakes, hypolimnetic dissolved oxygen was negatively correlated with diazotroph biovolume, possibly due to the release of nutrients, like phosphate and iron, bound to sediments. In addition, zooplankton grazers were negatively related to diazotrophic cyanobacteria biovolume, potentially reflecting the influence of stocked fish. Thus, lake management must account for bottom‐up factors, such as nutrient loading, which is influenced by lake morphometry and watershed size, as well as top‐down factors, such as fish stocking, to effectively mitigate diazotrophic cyanobacterial blooms.

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Phosphorus strategy in bloom-forming cyanobacteria (Dolichospermum and Microcystis) and its role in their succession

Cited by 86 publications

References 59 publications

Community composition and functional genes as well as ecological role of bacteria attached to different bloom-forming cyanobacteria

Community composition and functional genes as well as ecological role of bacteria attached to different bloom-forming cyanobacteria

Strategies adopted by Aphanizomenon flos-aquae in response to phosphorus deficiency and their role on growth

Watershed, lake, and food web factors influence diazotrophic cyanobacteria in mountain lakes

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