Phosphorylation-Coupled Proteolysis of the Transcription Factor MYC2 Is Important for Jasmonate-Signaled Plant Immunity

Zhai, Qingzhe; Yan, Liuhua; Tan, Dan; Chen, Rong; Sun, Jiaqiang; Gao, Liyan; Dong, Meng‐Qiu; Wang, Yingchun; Li, Chuanyou

doi:10.1371/journal.pgen.1003422

Cited by 185 publications

(192 citation statements)

References 48 publications

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“…As shown in Figures 7A and 7B, after inoculation with spores of B. cinerea, the myc2 mutant exhibited disease resistance, as indicated by the smaller lesion size compared with the wild type, which is similar with previous studies demonstrating that MYC2 negatively regulates resistance against B. cinerea (Lorenzo et al, 2004;Zhai et al, 2013). The ein3 eil1 (C) Co-IP assay to verify the interaction of MYC2 with EIN3 in planta.…”

Section: Disruption Of Ein3 and Eil1 Suppresses Exaggerated Apical Hosupporting

confidence: 85%

“…Surprisingly, MYC2 also acts as a negative regulator to repress JA-mediated plant resistance to necrotrophic fungi and pathogenesis-related gene expression (e.g., PDF1.2) (Anderson et al, 2004;Lorenzo et al, 2004;Zhai et al, 2013). Such MYC2-regulated susceptibility to necrotrophic fungi seems incompatible with the previously reported synergistic model (Zhu et al, 2011).…”

Section: Discussionmentioning

confidence: 82%

“…benthamiana leaves were infiltrated with Agrobacterium strains harboring flag-EIN3, flag-EIN3 with myc-MYC2 (Zhai et al, 2013), or flag-EIN3 with myc-COI1 . Two days after infiltration, 3 g of agroinfiltrated leaves for each combination was collected and homogenized in Co-IP buffer containing 50 mM Tris-HCl, pH 7.5, 100 mM NaCl, 2 mM DTT, 0.1% Tween 20, 1 mM phenylmethylsulfonyl fluoride, 50 mM MG132, and complete protease inhibitor cocktail (Roche) and centrifuged twice at 16,000g at 4°C.…”

Section: Co-ipmentioning

confidence: 99%

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Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

et al. 2014

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Plants have evolved sophisticated mechanisms for integration of endogenous and exogenous signals to adapt to the changing environment. Both the phytohormones jasmonate (JA) and ethylene (ET) regulate plant growth, development, and defense. In addition to synergistic regulation of root hair development and resistance to necrotrophic fungi, JA and ET act antagonistically to regulate gene expression, apical hook curvature, and plant defense against insect attack. However, the molecular mechanism for such antagonism between JA and ET signaling remains unclear. Here, we demonstrate that interaction between the JA-activated transcription factor MYC2 and the ET-stabilized transcription factor ETHYLENE-INSENSITIVE3 (EIN3) modulates JA and ET signaling antagonism in Arabidopsis thaliana. MYC2 interacts with EIN3 to attenuate the transcriptional activity of EIN3 and repress ET-enhanced apical hook curvature. Conversely, EIN3 interacts with and represses MYC2 to inhibit JA-induced expression of wound-responsive genes and herbivory-inducible genes and to attenuate JA-regulated plant defense against generalist herbivores. Coordinated regulation of plant responses in both antagonistic and synergistic manners would help plants adapt to fluctuating environments.

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Section: Disruption Of Ein3 and Eil1 Suppresses Exaggerated Apical Hosupporting

confidence: 85%

Section: Discussionmentioning

confidence: 82%

Section: Co-ipmentioning

confidence: 99%

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Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

et al. 2014

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“…MYC2, MYC3, and MYC4 are stabilized by light, via the action of red and blue light photoreceptors, and also by JA treatment (Chico et al, 2014). Phosphorylationdependent proteolysis of MYC2 was reported to be essential for its transcriptional activity (Zhai et al, 2013). In all these cases, MYC protein degradation was mediated by the proteasome.…”

Section: Discussionmentioning

confidence: 99%

Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract

et al. 2016

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Arabidopsis (Arabidopsis thaliana) plants recognize insect eggs and activate the salicylic acid (SA) pathway. As a consequence, expression of defense genes regulated by the jasmonic acid (JA) pathway is suppressed and larval performance is enhanced. Cross talk between defense signaling pathways is common in plant-pathogen interactions, but the molecular mechanism mediating this phenomenon is poorly understood. Here, we demonstrate that egg-induced SA/JA antagonism works independently of the APETALA2/ETHYLENE RESPONSE FACTOR (AP2/ERF) transcription factor ORA59, which controls the ERF branch of the JA pathway. In addition, treatment with egg extract did not enhance expression or stability of JASMONATE ZIM-domain transcriptional repressors, and SA/JA cross talk did not involve JASMONATE ASSOCIATED MYC2-LIKEs, which are negative regulators of the JA pathway. Investigating the stability of MYC2, MYC3, and MYC4, three basic helix-loop-helix transcription factors that additively control jasmonate-related defense responses, we found that egg extract treatment strongly diminished MYC protein levels in an SA-dependent manner. Furthermore, we identified WRKY75 as a novel and essential factor controlling SA/JA cross talk. These data indicate that insect eggs target the MYC branch of the JA pathway and uncover an unexpected modulation of SA/JA antagonism depending on the biological context in which the SA pathway is activated.

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“…Considering that MYC2 regulates diverse aspects of JA responses (Boter et al, 2004;Lorenzo et al, 2004;Dombrecht et al, 2007;Zhai et al, 2013), we examined whether this master regulator is also involved in COI1-mediated regulation of flowering time. Interestingly, in both LD and SD conditions, the flowering time of the myc2-2 mutant, which harbors a T-DNA insertion that disrupts the function of MYC2 (Boter et al, 2004), was comparable to its wild-type counterpart ( Figures 1A to 1E), indicating that MYC2 is not involved in COI1-dependent regulation of flowering time.…”

Section: Coi1-dependent Signaling Pathway Delays Flowering Of Arabidomentioning

confidence: 99%

Transcriptional Mechanism of Jasmonate Receptor COI1-Mediated Delay of Flowering Time in Arabidopsis

Zhai¹,

Zhang²,

Wu³

et al. 2015

Plant Cell

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Flowering time of plants must be tightly regulated to maximize reproductive success. Plants have evolved sophisticated signaling network to coordinate the timing of flowering in response to their ever-changing environmental conditions. Besides being a key immune signal, the lipid-derived plant hormone jasmonate (JA) also regulates a wide range of developmental processes including flowering time. Here, we report that the CORONATINE INSENSITIVE1 (COI1)-dependent signaling pathway delays the flowering time of Arabidopsis thaliana by inhibiting the expression of the florigen gene FLOWERING LOCUS T (FT). We provide genetic and biochemical evidence that the APETALA2 transcription factors (TFs) TARGET OF EAT1 (TOE1) and TOE2 interact with a subset of JAZ (jasmonate-ZIM domain) proteins and repress the transcription of FT. Our results support a scenario that, when plants encounter stress conditions, bioactive JA promotes COI1-dependent degradation of JAZs. Degradation of the JAZ repressors liberates the transcriptional function of the TOEs to repress the expression of FT and thereby triggers the signaling cascades to delay flowering. Our study identified interacting pairs of TF and JAZ transcriptional regulators that underlie JA-mediated regulation of flowering, suggesting that JA signals are converted into specific context-dependent responses by matching pairs of TF and JAZ proteins.

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Phosphorylation-Coupled Proteolysis of the Transcription Factor MYC2 Is Important for Jasmonate-Signaled Plant Immunity

Cited by 185 publications

References 48 publications

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

Interaction between MYC2 and ETHYLENE INSENSITIVE3 Modulates Antagonism between Jasmonate and Ethylene Signaling inArabidopsis

Arabidopsis MYC Transcription Factors Are the Target of Hormonal Salicylic Acid/Jasmonic Acid Cross Talk in Response to Pieris brassicae Egg Extract

Transcriptional Mechanism of Jasmonate Receptor COI1-Mediated Delay of Flowering Time in Arabidopsis

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