Phosphorylation influences water and ion channel function of <scp>AtPIP2;1</scp>

Qiu, Jiaen; McGaughey, Samantha; Groszmann, Michael; Tyerman, Stephen D.; Byrt, Caitlin S.

doi:10.1111/pce.13851

Cited by 50 publications

(63 citation statements)

References 86 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4B) is an outcome of changes in stomata and/or PD reactions, changes in ion fluxes or pH, and/or other unknown at present mechanisms that shape or block the systemic hydraulic signal and require GLR3.3;GLR3.6. One possible mechanism could be mediated through the function of aquaporins that are actively regulated in plants by phosphorylation, calcium, and/or other reactions (33,(57)(58)(59)(60). If for example a particular signal such as wounding would trigger a GLR3.3;GLR3.6-dependent signaling mechanism that will cause the simultaneous closure of aquaporins in cells, at and around the vascular bundle, this process could cause an increase in hydraulic pressure in the xylem vessels; because the pressure that is usually relieved from the xylem column into neighboring cells will now cease to be relieved.…”

Section: Discussionmentioning

confidence: 99%

Integration of electric, calcium, reactive oxygen species and hydraulic signals during rapid systemic signaling in plants

Fichman

Mittler

2021

Preprint

View full text Add to dashboard Cite

The sensing of abiotic stress, mechanical injury, or pathogen attack by a single plant tissue results in the activation of systemic signals that travel from the affected tissue to the entire plant, alerting it of an impending stress or pathogen attack. This process is essential for plant survival during stress and is termed systemic signaling. Among the different signals triggered during this process are calcium, electric, reactive oxygen species (ROS) and hydraulic signals. These are thought to propagate at rapid rates through the plant vascular bundles and to regulate many of the systemic processes essential for plant survival. Although the different signals activated during systemic signaling are thought to be interlinked, their coordination and hierarchy remain to be determined. Here, using a combination of advanced whole-plant imaging and hydraulic pressure measurements, we studied the activation of all four systemic signals in wild type and different Arabidopsis thaliana mutants subjected to a local high light (HL) stress or wounding. Our findings reveal that in response to wounding systemic changes in membrane potential, calcium, ROS, and hydraulic pressure are coordinated by glutamate receptor-like (GLR) proteins 3.3 and 3.6, while in response to HL the respiratory burst oxidase homolog D-driven systemic ROS signal could be separated from systemic changes in membrane potential and calcium levels. We further determine that plasmodesmata functions are required for systemic changes in membrane potential, calcium, and ROS during systemic signaling. Our findings shed new light on the different mechanisms that integrate different systemic signals in plants during stress.

show abstract

Section: Discussionmentioning

confidence: 99%

Integration of electric, calcium, reactive oxygen species and hydraulic signals during rapid systemic signaling in plants

Fichman

Mittler

2021

Preprint

View full text Add to dashboard Cite

show abstract

“…None of the three NtPIP1s examined here enhanced permeability to water, but were permeable to H 2 O 2 (NtPIP1;1t) or BA (NtPIP1;5s) (Table 1). As permeability to H 2 O 2 and BA was not predicted for these PIP1s by Ahmed et al (2020), subtle sequence differences or pore dynamics of these isoforms clearly can modify an AQP’s preferential transport to a given substrate (Qiu et al 2020). Various PIP1 and 2 isoforms from other species are permeable to BA (e.g.…”

Section: Discussionmentioning

confidence: 99%

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

Evans

Groszmann

2021

Preprint

Self Cite

View full text Add to dashboard Cite

Aquaporins (AQPs) are involved in a variety of vital plant physiological processes, including water relations, development, stress responses and photosynthesis. Nicotiana tabacum (tobacco) has 84 AQP genes due to its recent hybridisation and allotetraploid genome. We functionally characterised a diverse subset of tobacco AQPs spanning the 3 largest AQP subfamilies, selecting nine isoforms from the PIPs, TIPs, and NIPs. Using high-throughput yeast-based functional assays, we determined AQP permeability to water, hydrogen peroxide (H2O2), boric acid (BA) and urea. For each AQP, subcellular localisations in planta were established using GFP translational fusions. From 3D protein homology modelling, we found that the monomeric pore shape/size, selectivity filter region and NPA motifs is insufficient to comprehensively predict their transport capabilities. PIPs had the narrowest pore diameter and were permeable to water, H2O2 and BA. The pore in TIPs was wider and more cylindrical in shape than for the PIPs. TIP1;1s was permeable to all four substrates tested, and is highly expressed in leaves and flowers, suggesting it functions in multiple roles. By contrast, NIP5;1t, with a larger pore size than the NtTIPs, is only expressed in young flowers and enhanced permeability only to BA. Its homolog in Arabidopsis (AtNIP5;1) has the same substrate specificity and functions as a boron channel.

show abstract

“…The phosphorylation of PIP channels is considered as the key mechanism for cell water flow regulation in response to environmental clues [43]. The phosphorylation of two serine residues on AtPIP2.1 was recently demonstrated to regulate the activity of this channel to shift between passage of water and ions [44]. Higher water absorption of rice grown in ammonium is connected with up-regulation of aquaporin genes [4,6].…”

Section: Discussionmentioning

confidence: 99%

The phosphoproteome of rice leaves responds to water and nitrogen supply

Hamzelou

Melino

Plett

et al. 2021

Preprint

View full text Add to dashboard Cite

The scarcity of freshwater is an increasing concern in flood-irrigated rice, whilst excessive use of nitrogen fertilizers is both costly and contributes to environmental pollution. To co-ordinate growth adaptation under prolonged exposure to limited water or excess nitrogen supply, plants have processes for signalling and regulation of metabolic processes. There is limited information on the involvement of one of the most important post-translational modifications (PTMs), protein phosphorylation, on plant adaptation to long-term changes in resource supply. Oryza sativa cv. Nipponbare was grown under two regimes of nitrogen from the time of germination to final harvest. Twenty-five days after germination, water was withheld from half the pots in each nitrogen treatment and low water supply continued for an additional 26 days, while the remaining pots were well watered. Leaves from all four groups of plants were harvested after 51 days in order to test whether phosphorylation of leaf proteins responded to prior abiotic events. The dominant impact of these resources is exerted in leaves, where PTMs have been predicted to occur. Proteins were extracted and phosphopeptides were analysed by nanoLC-MS/MS analysis, coupled with label-free quantitation. Water and nitrogen regimes triggered extensive changes in phosphorylation of proteins involved in membrane transport, such as the aquaporin OsPIP2-6, a water channel protein. Our study reveals phosphorylation of several peptides belonging to proteins involved in RNA-processing and carbohydrate metabolism, suggesting that phosphorylation events regulate the signalling cascades that are required to optimize plant response to resource supply.

show abstract

Phosphorylation influences water and ion channel function of AtPIP2;1

Cited by 50 publications

References 86 publications

Integration of electric, calcium, reactive oxygen species and hydraulic signals during rapid systemic signaling in plants

Integration of electric, calcium, reactive oxygen species and hydraulic signals during rapid systemic signaling in plants

Exploring the Plant Aquaporin Solute Transport Network: Functional characterisation ofNicotiana tabacumPIP, TIP and NIP isoforms

The phosphoproteome of rice leaves responds to water and nitrogen supply

Contact Info

Product

Resources

About