1997
DOI: 10.1074/jbc.272.45.28252
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Phosphorylation of Elongation Factor 1 and Ribosomal Protein S6 by Multipotential S6 Kinase and Insulin Stimulation of Translational Elongation

Abstract: Stimulation of protein synthesis in response to insulin is concomitant with increased phosphorylation of initiation factors 4B and 4G and ribosomal protein S6 (Morley, S. J., and Traugh, J. A. (1993) Biochimie 75, 985-989) and is due at least in part to multipotential S6 kinase. When elongation factor 1 (EF-1) from rabbit reticulocytes was examined as substrate for multipotential S6 kinase, up to 1 mol/mol of phosphate was incorporated into the ␣, ␤, and ␦ subunits. Phosphorylation of EF-1 resulted in a 2-2.6-… Show more

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Cited by 48 publications
(42 citation statements)
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“…Overall phosphorylation is significantly decreased under conditions of stress. Serum deprivation reduces translation and phosphorylation of eIF4B, ribosomal protein S6, and elongation factor 1 (41)(42)(43). Incubation of cells at elevated temperatures (43-44°C) for 20 min causes an inhibition of translation with concomitant dephosphorylation of eIF4B and eIF4E (44 -46) and increased binding of 4E-BP1 to eIF4E (47).…”
Section: Discussionmentioning
confidence: 99%
“…Overall phosphorylation is significantly decreased under conditions of stress. Serum deprivation reduces translation and phosphorylation of eIF4B, ribosomal protein S6, and elongation factor 1 (41)(42)(43). Incubation of cells at elevated temperatures (43-44°C) for 20 min causes an inhibition of translation with concomitant dephosphorylation of eIF4B and eIF4E (44 -46) and increased binding of 4E-BP1 to eIF4E (47).…”
Section: Discussionmentioning
confidence: 99%
“…Many agents that raise intracellular Ca 2ϩ concentrations also bring about eEF2 phosphorylation, including histamine treatment of epithelial cells (92,93), or glutamate or N-methyl-D-aspartate (NMDA) treatment of neurons (see below). Conversely, insulin stimulation leads to eEF2 dephosphorylation, resulting in a decrease in ribosomal transit time, and an increase in elongation rates (94)(95)(96). Rapamycin treatment inhibits the insulin-stimulated dephosphorylation of eEF2 (91,96).…”
Section: E-bpsmentioning
confidence: 99%
“…The phosphorylation leads to the stimulation of poly(U)-dependent polyphenylalanine synthesis. 53) Since the TOP includes an oligo(U) sequence, phosphorylated S6 is thought to stimulate polyphenylalanine synthesis by the same mechanism that mediates the preferential translation of TOP mRNAs.…”
Section: )mentioning
confidence: 99%