Phosphorylation of endogenous and TEST-yolk buffer proteins by intact human sperm

Miceli, Dora C.; Llanos, Ricardo J.; Dı́az, Maykelis; Peralta, Luisa

doi:10.1017/s0967199498000136

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…This change was not observed in the samples cooled with AM-EY, despite the basic extender being the same, so possibly this difference could be attributed to the presence of EY in the medium, as it has been hypothesized that egg yolk would induce llama sperm, obtained by electroejaculation, to attain progressive motility (11,27). Likewise, Miceli et al (28) observed that specific surface proteins on human spermatozoa were phosphorylated to a greater extent in sperm incubated with TEST buffer with egg yolk (TYB) than those incubated in other media without EY. Results of this study also indicated that TYB proteins could also be phosphorylated by the spermatozoa during the incubation and attach to the sperm membranes.…”

Section: Discussionmentioning

confidence: 93%

Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts

et al. 2020

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The use of non-commercial and commercial extenders for cooling alpaca sperm has already been reported, the latter showing certain advantages over the first. The Andromed® (AM) extender was created for use in ruminants and has also been tested in ejaculated and epididymal alpaca sperm. According to the manufacturer, this extender does not need the addition of egg yolk (EY); however, it is known that the addition of EY to some extenders improves the preservation of cooled sperm. The objective of this study therefore was to compare a non-commercial extender (Tris) with the addition of EY vs. the commercial extender AM with and without the addition of EY, for cooling alpaca sperm obtained from diverted deferent ducts. Fifteen pools of deferent duct sperm were formed using samples from two or three different males for each. Each sperm pool was evaluated and then divided into three aliquots that were diluted to a final concentration of 30 × 106 sperm ml-1 (0 h) with either: (1) Tris with 20% EY (T-EY), (2) AM, or (3) AM with 20% EY (AM-EY). Samples were cooled to 5°C and the following sperm parameters were evaluated after 24 and 48 h of storage: motility, viability, membrane function, acrosome integrity, morphology, and chromatin condensation. Motility was also evaluated after 72 h of storage. The samples that best preserved progressive and total sperm motility at the 24 and 48 h evaluation periods were the ones diluted with AM-EY, observing that with this extender these motility patterns decreased significantly after 72 h of storage compared to time 0 h (p < 0.05). A significant decrease (p < 0.05) in total and progressive motility was observed at 48 h for the T-EY and AM extender compared to 0 h. AM was the only extender in which the percentages of viable sperm decreased significantly (p < 0.05) after 48 h of conservation. For the rest of sperm parameters evaluated, no significant differences were observed between any of the extenders at any evaluation time. The Andromed® extender with the addition of 20% EY could be an alternative option for cooling alpaca sperm obtained from deferent ducts.

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Section: Discussionmentioning

confidence: 93%

Comparison of Extenders With the Addition of Egg Yolk for Cooling Alpaca Sperm Obtained From Deferent Ducts

et al. 2020

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“…These results would seem to indicate that the presence of egg yolk in the extender at 37 • C would maintain sperm motility in this species. To be able to explain the effect of the egg yolk in the extender on sperm motility, Miceli et al (1998) examined the effect of preserving human semen in the TEST buffer with egg yolk (TYB). These authors observed that specific surface proteins on spermatozoa were phosphorylated to a greater extent in sperm incubated with TYB than those incubated in other media.…”

Section: Discussionmentioning

confidence: 99%