Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Saraf, Amit; Luo, Jie; Morris, David R.; Storm, Daniel R.

doi:10.1074/jbc.m114.552638

Cited by 27 publications

(31 citation statements)

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“…Although rhythmic coordination of transcription and mRNA accumulation has been extensively studied during the last decades, rhythmic translation has been described only recently in mammals. Indeed, it has been shown that the TORC1 pathway is rhythmically activated in mouse liver (12,49), hippocampus (50), and SCN (51), influencing in this way rhythmic ribosome biogenesis (12) or light response and circadian behavior (52,53). Moreover, the circadian clock, in coordination with feeding rhythms, seems to play a modulating role in this activation (12,54,55).…”

Section: Translation Efficiency Is Regulated During the Diurnal Cyclementioning

confidence: 99%

Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver

Atger

Gobet

Marquis

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

217

287

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Diurnal oscillations of gene expression are a hallmark of rhythmic physiology across most living organisms. Such oscillations are controlled by the interplay between the circadian clock and feeding rhythms. Although rhythmic mRNA accumulation has been extensively studied, comparatively less is known about their transcription and translation. Here, we quantified simultaneously temporal transcription, accumulation, and translation of mouse liver mRNAs under physiological light-dark conditions and ad libitum or night-restricted feeding in WT and brain and muscle Arnt-like 1 (Bmal1)-deficient animals. We found that rhythmic transcription predominantly drives rhythmic mRNA accumulation and translation for a majority of genes. Comparison of wild-type and Bmal1 KO mice shows that circadian clock and feeding rhythms have broad impact on rhythmic gene expression, Bmal1 deletion affecting surprisingly both transcriptional and posttranscriptional levels. Translation efficiency is differentially regulated during the diurnal cycle for genes with 5′-Terminal Oligo Pyrimidine tract (5′-TOP) sequences and for genes involved in mitochondrial activity, many harboring a Translation Initiator of Short 5′-UTR (TISU) motif. The increased translation efficiency of 5′-TOP and TISU genes is mainly driven by feeding rhythms but Bmal1 deletion also affects amplitude and phase of translation, including TISU genes. Together this study emphasizes the complex interconnections between circadian and feeding rhythms at several steps ultimately determining rhythmic gene expression and translation.circadian rhythms | ribosome profiling | mRNA translation | 5′-TOP sequences | TISU motifs L iving organisms on Earth are subjected to light-dark cycles caused by rotation of the Earth around the sun. To anticipate these changes, virtually all organisms have acquired a circadian timing system during evolution that allows a better adaptation to their environment. As a consequence, most aspects of their physiology are orchestrated in a rhythmic way by the circadian clock (from the Latin circa diem, meaning "about a day"), an endogenous and autonomous oscillator with a period of around 24 h (1, 2). Not surprisingly, perturbations of this clock in mammals lead to pathologies including psychiatric, metabolic, and vascular disorders (1,3,4). At the organismal scale, the oscillatory clockwork is organized in a hierarchal manner. Within the suprachiasmatic nuclei (SCN) of the hypothalamus, the "master clock" receives light input via the retina and communicates timing signals to "enslave" oscillators in peripheral organs (1, 2). The molecular oscillator consists of interconnected transcriptional and translational feedback loops, in which multiple layers of control, including temporal posttranscriptional and posttranslational regulation, play important roles (5). These additional layers of regulation are largely coordinated by systemic cues originating from circadian clock and/or feeding-coordinated rhythmic metabolism, allowing the adjustment of the molecular clo...

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Section: Translation Efficiency Is Regulated During the Diurnal Cyclementioning

confidence: 99%

Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver

Atger

Gobet

Marquis

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

217

287

View full text Add to dashboard Cite

show abstract

“…Phosphorylation and activation of eIF4E-binding protein 1 (4EBP1) and ribosomal protein S6 are also markers for translation initiation. Therefore, the initiation of translation was measured by monitoring p-EIF4E, p-4EBP1 and Page 18 of 41 A c c e p t e d M a n u s c r i p t 18 pS6 30 minutes after training for contextual fear memory (Saraf et al 2014). Context trained mice but not unpaired controls show significant increases in p-EIF4E, p-4EBP1 and pS6 indicating an increase in protein synthesis in area CA1 following training (Fig.4 ).…”

Section: Initiation Of Protein Synthesis Undergoes Diurnal Oscillatiomentioning

confidence: 98%

“…M a n u s c r i p t 22 A c c e p t e d M a n u s c r i p t 25 4hr. Data is shown as mean ± sem, *** p<0.001 ZT4 hr vs. ZT16 hr using student's t-test (Saraf et al 2014). Step-through latency in passive avoidance.…”

Section: Conflict Of Interestmentioning

confidence: 99%

“…Step-through latency in passive avoidance. Data is expressed as mean ± sem, *** p<0.001, ** p<0.01 vehicle vs. anisomycin group using student's t-test (Saraf et al 2014). A c c e p t e d M a n u s c r i p t A c c e p t e d M a n u s c r i p t 28 M a n u s c r i p t…”

Section: Conflict Of Interestmentioning

confidence: 99%

“…Data is Mean ± Standard error of mean (sem), and is expressed as a percentage of control (shock). N (number of mice) is 5 each for control and context-shock vs. control (shock) using student's t-test(Saraf et al 2014).…”

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Role of circadian rhythm and REM sleep for memory consolidation

Xia

Storm

2017

Neuroscience Research

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Although sleep is strongly implicated in memory consolidation, the molecular basis for the role of sleep in memory is not known. It has been established that the consolidation of hippocampus-dependent memory depends on the activation of the Erk1,2 MAP kinase (MAPK) pathway which activates de novo CRE-mediated transcription and translation, two processes required for memory consolidation pathway. The activation of MAPK during memory formation and its nuclear translocation both depend upon cAMP signals generated by the calmodulin-stimulated adenylyl cyclases, type 1 and type 8 (AC1 and AC8). This signaling pathway undergoes a circadian oscillation in the hippocampus with maximal activation during REM sleep. This data supports the hypothesis that the persistence of long-term memory traces may depend upon the reactivation and circadian oscillation of the cAMP/MAP kinase/CRE transcriptional pathway in tagged neurons which reaches a maximum during REM sleep.

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Aldosterone: Renal Action and Physiological Effects

Johnston

Welch

Cain

et al. 2023

Comprehensive Physiology

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Aldosterone exerts profound effects on renal and cardiovascular physiology. In the kidney, aldosterone acts to preserve electrolyte and acid-base balance in response to changes in dietary sodium (Na + ) or potassium (K + ) intake. These physiological actions, principally through activation of mineralocorticoid receptors (MRs), have important effects particularly in patients with renal and cardiovascular disease as demonstrated by multiple clinical trials. Multiple factors, be they genetic, humoral, dietary, or otherwise, can play a role in influencing the rate of aldosterone synthesis and secretion from the adrenal cortex. Normally, aldosterone secretion and action respond to dietary Na + intake. In the kidney, the distal nephron and collecting duct are the main targets of aldosterone and MR action, which stimulates Na + absorption in part via the epithelial Na + channel (ENaC), the principal channel responsible for the fine-tuning of Na + balance. Our understanding of the regulatory factors that allow aldosterone, via multiple signaling pathways, to function properly clearly implicates this hormone as central to many pathophysiological effects that become dysfunctional in disease states. Numerous pathologies that affect blood pressure (BP), electrolyte balance, and overall cardiovascular health are due to abnormal secretion of aldosterone, mutations in MR, ENaC, or effectors and modulators of their action. Study of the mechanisms of these pathologies has allowed researchers and clinicians to create novel dietary and pharmacological targets to improve human health. This article covers the regulation of aldosterone synthesis and secretion, receptors, effector molecules, and signaling pathways that modulate its action in the kidney. We also consider the role of aldosterone in disease and the benefit of mineralocorticoid antagonists.

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Phosphorylation of Eukaryotic Translation Initiation Factor 4E and Eukaryotic Translation Initiation Factor 4E-binding Protein (4EBP) and Their Upstream Signaling Components Undergo Diurnal Oscillation in the Mouse Hippocampus

Cited by 27 publications

References 54 publications

Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver

Circadian and feeding rhythms differentially affect rhythmic mRNA transcription and translation in mouse liver

Role of circadian rhythm and REM sleep for memory consolidation

Aldosterone: Renal Action and Physiological Effects

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