Phosphorylation of Phosphoenolpyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO2 Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species Kalanchoë fedtschenkoi

Boxall, Susanna Flavia; Dever, Louisa V.; Kneřová, Jana; Gould, Peter; Hartwell, James

doi:10.1105/tpc.17.00301

Cited by 70 publications

(119 citation statements)

References 60 publications

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“…Hence, the light/dark synchronization of the PPC-mediated primary carboxylation reactions in C 4 -and CAM-performing leaves may, at least partially, rely on the opposite diel transcriptional regulation of these two PPC genes (Fig. However, PPC is also post-translationally regulated, with PPCK-mediated PPC phosphorylation during the daytime (C 4 ) or night-time (CAM), adjusting the enzyme's sensitivity to its allosteric inhibitor L-malate over the diel cycle (Nimmo et al, 2001;Hibberd & Covshoff, 2010;Boxall et al, 2017). However, PPC is also post-translationally regulated, with PPCK-mediated PPC phosphorylation during the daytime (C 4 ) or night-time (CAM), adjusting the enzyme's sensitivity to its allosteric inhibitor L-malate over the diel cycle (Nimmo et al, 2001;Hibberd & Covshoff, 2010;Boxall et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

Ferrari¹,

Bittencourt²,

Rodrigues³

et al. 2019

New Phytologist

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Although biochemically related, C 4 and crassulacean acid metabolism (CAM) systems are expected to be incompatible. However, Portulaca species, including P. oleracea, operate C 4 and CAM within a single leaf, and the mechanisms behind this unique photosynthetic arrangement remain largely unknown.Here, we employed RNA-seq to identify candidate genes involved exclusively or shared by C 4 or CAM, and provided an in-depth characterization of their transcript abundance patterns during the drought-induced photosynthetic transitions in P. oleracea. Data revealed fewer candidate CAM-specific genes than those recruited to function in C 4 . The putative CAMspecific genes were predominantly involved in night-time primary carboxylation reactions and malate movement across the tonoplast. Analysis of gene transcript-abundance regulation and photosynthetic physiology indicated that C 4 and CAM coexist within a single P. oleracea leaf under mild drought conditions. Developmental and environmental cues were shown to regulate CAM expression in stems, whereas the shift from C 4 to C 4 -CAM hybrid photosynthesis in leaves was strictly under environmental control. Moreover, efficient starch turnover was identified as part of the metabolic adjustments required for CAM operation in both organs.These findings provide insights into C 4 /CAM connectivity and compatibility, contributing to a deeper understanding of alternative ways to engineer CAM into C 4 crop species.

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Section: Discussionmentioning

confidence: 99%

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

Ferrari¹,

Bittencourt²,

Rodrigues³

et al. 2019

New Phytologist

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“…These early reports would later be supported by data on how this process is regulated by the clock in Kalanchoë at the transcriptional level (Boxall et al . ; Yang et al . ).…”

Section: The Molecular Clockmentioning

confidence: 99%

On the move through time – a historical review of plant clock research

Johansson

Köster

2018

Plant Biol J

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The circadian clock is an important regulator of growth and development that has evolved to help organisms to anticipate the predictably occurring events on the planet, such as light-dark transitions, and adapt growth and development to these. This review looks back in history on how knowledge about the endogenous biological clock has been acquired over the centuries, with a focus on discoveries in plants. Key findings at the physiological, genetic and molecular level are described and the role of the circadian clock in important molecular processes is reviewed.

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“…This is supported by the analysis of the pineapple ( Ananas comosus ) genome showing that CAM has evolved from re‐wiring of existing C3 components through regulatory neofunctionalisation, rather than by acquisition of new genes with specialised CAM functions through duplication processes (Ming et al , ) . Additional evidence comes from the extent that the circadian expression of the CAM cycle can be disrupted when key metabolic enzymes have been manipulated (Dever et al , ; Hartwell et al , ; Boxall et al , ). These are explored in more detail in a subsequent section of this review.…”

Section: Introductionmentioning

confidence: 99%

Model approaches to advance crassulacean acid metabolism system integration

Chomthong

Griffiths

2020

The Plant Journal

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Summary This review summarises recent progress in understanding crassulacean acid metabolism (CAM) systems and the integration of internal and external stimuli to maximise water‐use efficiency. Complex CAM traits have been reduced to their minimum and captured as computational models, which can now be refined using recently available data from transgenic manipulations and large‐scale omics studies. We identify three key areas in which an appropriate choice of modelling tool could help capture relevant comparative molecular data to address the evolutionary drivers and plasticity of CAM. One focus is to identify the environmental and internal signals that drive inverse stomatal opening at night. Secondly, it is important to identify the regulatory processes required to orchestrate the diel pattern of carbon fluxes within mesophyll layers. Finally, the limitations imposed by contrasting succulent systems and associated hydraulic conductance components should be compared in the context of water‐use and evolutionary strategies. While network analysis of transcriptomic data can provide insights via co‐expression modules and hubs, alternative forms of computational modelling should be used iteratively to define the physiological significance of key components and informing targeted functional gene manipulation studies. We conclude that the resultant improvements of bottom‐up, mechanistic modelling systems can enhance progress towards capturing the physiological controls for phylogenetically diverse CAM systems in the face of the recent surge of information in this omics era.

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Phosphorylation of Phosphoenolpyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO₂ Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species Kalanchoë fedtschenkoi

Cited by 70 publications

References 60 publications

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

On the move through time – a historical review of plant clock research

Model approaches to advance crassulacean acid metabolism system integration

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Phosphorylation of Phosphoenolpyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO2 Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species Kalanchoë fedtschenkoi

Cited by 70 publications

References 60 publications

C4 and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

C4 and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

On the move through time – a historical review of plant clock research

Model approaches to advance crassulacean acid metabolism system integration

Contact Info

Product

Resources

About

Phosphorylation of Phosphoenolpyruvate Carboxylase Is Essential for Maximal and Sustained Dark CO₂ Fixation and Core Circadian Clock Operation in the Obligate Crassulacean Acid Metabolism Species Kalanchoë fedtschenkoi

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation

C₄ and crassulacean acid metabolism within a single leaf: deciphering key components behind a rare photosynthetic adaptation