Phosphorylation of protein kinase B, the key enzyme in insulin-signaling cascade, is enhanced in linoleic and arachidonic acid–treated HT29 and HepG2 cells

Mariniello, Katia; Min, Yoeju; Ghebremeskel, Kebreab

doi:10.1016/j.nut.2018.05.033

Cited by 5 publications

(2 citation statements)

References 46 publications

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“…Walnuts are rich source of LA, while arachidonic acid is its metabolite (Croitoru et al 2019). Our finding that walnut diet increased Akt phosphorylation is in concordance with the data showing that phosphorylation of Akt is enhanced in LA and arachidonic acid-treated HT29 and HepG2 cells (Mariniello et al 2019). Hennig et al (2006) suggests that both PI3K/Akt-and ERK1/2-mediated proinflammatory signalling events are critical in LA-induced endothelial cell activation and vascular inflammation.…”

Section: Protein Kinasessupporting

confidence: 90%

Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Stanišić

Ivković

Romić

et al. 2020

International Journal of Food Sciences and Nutrition

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Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.

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Section: Protein Kinasessupporting

confidence: 90%

Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Stanišić

Ivković

Romić

et al. 2020

International Journal of Food Sciences and Nutrition

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“…The metabolic-related signaling pathways such as linoleic acid, arachidonic acid, arginine and proline metabolism were also enhanced in the HF mice with CLA supplemented. Through treating human liver hepatocellular (HepG2) cells with linoleic acid and arachidonic acid, respectively, Mariniello et al [ 41 ] found that these two polyunsaturated fatty acids substantially enhanced the insulin action by increasing the phosphorylation of AKT. Arginine is proposed to be the largest nitrogen-donating amino acid in the body and acts as the precursor of proline.…”

Section: Discussionmentioning

confidence: 99%

Conjugated Linoleic Acid Ameliorates High Fat-Induced Insulin Resistance via Regulating Gut Microbiota–Host Metabolic and Immunomodulatory Interactions

Wu,

Ye,

Deng

et al. 2024

Nutrients

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Interaction between gut microbiota, host immunity and metabolism has been suggested to crucially affect the development of insulin resistance (IR). This study aims to investigate how gut microbiota, inflammatory responses and metabolism in individuals with IR are affected by the supplementation of conjugated linoleic acid (CLA) and how this subsequently affects the pathophysiology of IR by using a high-fat diet-induced IR mouse model. Serum biochemical indices showed that 400 mg/kg body weight of CLA effectively attenuated hyperglycemia, hyperlipidemia, glucose intolerance and IR, while also promoting antioxidant capacities. Histomorphology, gene and protein expression analysis revealed that CLA reduced fat deposition and inflammation, and enhanced fatty acid oxidation, insulin signaling and glucose transport in adipose tissue or liver. Hepatic transcriptome analysis confirmed that CLA inhibited inflammatory signaling pathways and promoted insulin, PI3K-Akt and AMPK signaling pathways, as well as linoleic acid, arachidonic acid, arginine and proline metabolism. Gut microbiome analysis further revealed that these effects were highly associated with the enriched bacteria that showed positive correlation with the production of short-chain fatty acids (SCFAs), as well as the improved SCFAs production simultaneously. This study highlights the therapeutic actions of CLA on ameliorating IR via regulating microbiota–host metabolic and immunomodulatory interactions, which have important implications for IR control.

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Identifying Codium fragile extract components and their effects on muscle weight and exercise endurance

et al. 2021

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Phosphorylation of protein kinase B, the key enzyme in insulin-signaling cascade, is enhanced in linoleic and arachidonic acid–treated HT29 and HepG2 cells

Cited by 5 publications

References 46 publications

Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2

Conjugated Linoleic Acid Ameliorates High Fat-Induced Insulin Resistance via Regulating Gut Microbiota–Host Metabolic and Immunomodulatory Interactions

Identifying Codium fragile extract components and their effects on muscle weight and exercise endurance

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