Phosphorylation of Ribosomal Proteins Induced by Auxins in Maize Embryonic Tissues

Perez, Laura Collazo; Aguilar, Raúl; Méndez, Alma Pérez; Jiménez, Estela Sánchez de

doi:10.1104/pp.94.3.1270

Cited by 22 publications

(15 citation statements)

References 30 publications

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“…At the 4-h time point, genes encoding ribosomal proteins, translation initiation factors, and regulators of ribosomal biogenesis constituted the largest funcat of the induced genes, ''protein synthesis.'' Some of the ribosomal proteins have been shown to be directly regulated by developmental signals, such as auxin (34,35). Unlike transcriptional regulation during cell cycle, regulation of cell cyclespecific translation is poorly described (36).…”

Section: Resultsmentioning

confidence: 99%

Transcript profiling of early lateral root initiation

Himanen

Vuylsteke

Vanneste

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

190

161

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At the onset of lateral root initiation in Arabidopsis thaliana, the phytohormone auxin activates xylem pole pericycle cells for asymmetric cell division. However, the molecular events leading from auxin to lateral root initiation are poorly understood, in part because the few responsive cells in the process are embedded in the root and are thus difficult to access. A lateral root induction system, in which most xylem pole pericycle cells were synchronously activated by auxin transport inhibition followed by auxin application, was used for microarray transcript profiling. Of 4,600 genes analyzed, 906 significantly differentially regulated genes were identified that could be grouped into six major clusters. Basically, three major patterns were discerned representing induced, repressed, and transiently expressed genes. Analysis of the coregulated genes, which were specific for each time point, provided new insight into the molecular regulation and signal transduction preceding lateral root initiation in Arabidopsis. The reproducible expression profiles during a time course allowed us to define four stages that precede the cell division in the pericycle. These early stages were characterized by G 1 cell cycle block, auxin perception, and signal transduction, followed by progression over G 1͞S transition and G2͞M transition. All these processes took place within 6 h after transfer from N-1-naphthylphthalamic acid to 1-naphthalene acetic acid. These results indicate that this lateral root induction system represents a unique synchronized system that allows the systematic study of the developmental program upstream of the cell cycle activation during lateral root initiation.auxin ͉ cell cycle ͉ microarray

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Section: Resultsmentioning

confidence: 99%

Transcript profiling of early lateral root initiation

Himanen

Vuylsteke

Vanneste

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

190

161

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“…These mRNAs predominantly encode components of the translational apparatus that are required for cell growth and proliferation (Jefferies et al, 1994;Holland et al, 2004). In plants, the phosphorylation of S6 is developmentally programmed and regulated by temperature, oxygen availability, and other growth conditions (Scharf and Nover, 1982;Perez et al, 1990;Turck et al, 1998;Williams et al, 2003). The stimulation of S6 phosphorylation upon transfer of cultured Arabidopsis cells to fresh medium containing auxin and kinetin was correlated with increased association of 2 r-protein mRNAs with polysomes (Turck et al, 2004).…”

Section: Products Of Evolutionarily Distinct Gene Family Members Are mentioning

confidence: 99%

Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes

et al. 2005

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Analysis of 80S ribosomes of Arabidopsis (Arabidopsis thaliana) by use of high-speed centrifugation, sucrose gradient fractionation, one-and two-dimensional gel electrophoresis, liquid chromatography purification, and mass spectrometry (matrix-assisted laser desorption/ionization time-of-flight and electrospray ionization) identified 74 ribosomal proteins (r-proteins), of which 73 are orthologs of rat r-proteins and one is the plant-specific r-protein P3. Thirty small (40S) subunit and 44 large (60S) subunit r-proteins were confirmed. In addition, an ortholog of the mammalian receptor for activated protein kinase C, a tryptophan-aspartic acid-domain repeat protein, was found to be associated with the 40S subunit and polysomes. Based on the prediction that each r-protein is present in a single copy, the mass of the Arabidopsis 80S ribosome was estimated as 3.2 MD (1,159 kD 40S; 2,010 kD 60S), with the 4 single-copy rRNAs (18S, 26S, 5.8S, and 5S) contributing 53% of the mass. Despite strong evolutionary conservation in r-protein composition among eukaryotes, Arabidopsis 80S ribosomes are variable in composition due to distinctions in mass or charge of approximately 25% of the r-proteins. This is a consequence of amino acid sequence divergence within r-protein gene families and posttranslational modification of individual r-proteins (e.g. amino-terminal acetylation, phosphorylation). For example, distinct types of r-proteins S15a and P2 accumulate in ribosomes due to evolutionarily divergence of r-protein genes. Ribosome variation is also due to amino acid sequence divergence and differential phosphorylation of the carboxy terminus of r-protein S6. The role of ribosome heterogeneity in differential mRNA translation is discussed.The ribosome is a two-subunit ribonucleoprotein complex that catalyzes the peptidyl transferase reaction of polypeptide synthesis, an absolute requirement for cellular growth and differentiation. The structure and function of both prokaryotic and eukaryotic ribosomes have been investigated, with the eukaryotic emphasis on ribosomes of Baker's yeast (Saccharomyces cerevisiae) and rat (Rattus rattus and Rattus norvegicus). The cytosolic ribosomes of eukaryotes are composed of a large number of ribosomal proteins (r-proteins) and four distinct rRNAs, the 18S rRNA of the 40S subunit, and the 5S, 5.8S, and 23S-like (25-28S) rRNAs of the 60S subunit (Bielka, 1982). Early evaluation of the buoyant density and sedimentation coefficients of eukaryotic ribosomes predicted that the rat 80S ribosome has a higher mass (4.2-4.6 MD) than that of pea (Pisum sativum; 3.9 MD) due to distinctions in the 60S subunit (Cammarano et al., 1972). Further evidence that the plant ribosome is smaller than that of mammals was provided by the three-dimensional reconstruction of wheat (Triticum aestivum) and rabbit (Oryctolagus cuniculus) ribosomes by use of cryoelectron microscopy (Verschoor et al., 1996). Despite an overall similarity in architecture, the 60S subunit of wheat appeared approximately 20% smaller than that...

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“…Several ribosomal proteins have been associated with auxin-related developmental processes, such as cell proliferation, cell expansion, and polarity establishment in leaves (39)(40)(41). Auxins also trigger the coordinate expression of ribosomal protein mRNAs (55,56), increase de novo ribosomal protein synthesis (57), and change ribosomal protein phosphorylation patterns (58,59). In our study, mutations in multiple ribosomal genes caused specific auxin-related defects rather than a nonspecific defect in translation.…”

Section: Arf3 Uorfs Are Partially Responsible For Several Auxin-relatedmentioning

confidence: 99%

Arabidopsis ribosomal proteins control developmental programs through translational regulation of auxin response factors

Rosado

Ven

et al. 2012

Proc. Natl. Acad. Sci. U.S.A.

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Upstream ORFs are elements found in the 5′-leader sequences of specific mRNAs that modulate the translation of downstream ORFs encoding major gene products. In Arabidopsis, the translational control of auxin response factors (ARFs) by upstream ORFs has been proposed as a regulatory mechanism required to respond properly to complex auxin-signaling inputs. In this study, we identify and characterize the aberrant auxin responses in specific ribosomal protein mutants in which multiple ARF transcription factors are simultaneously repressed at the translational level. This characteristic lends itself to the use of these mutants as genetic tools to bypass the genetic redundancy among members of the ARF family in Arabidopsis. Using this approach, we were able to assign unique functions for ARF2, ARF3, and ARF6 in plant development.ribosomal mutants | uORFs | auxin regulation

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Phosphorylation of Ribosomal Proteins Induced by Auxins in Maize Embryonic Tissues

Cited by 22 publications

References 30 publications

Transcript profiling of early lateral root initiation

Transcript profiling of early lateral root initiation

Proteomic Characterization of Evolutionarily Conserved and Variable Proteins of Arabidopsis Cytosolic Ribosomes

Arabidopsis ribosomal proteins control developmental programs through translational regulation of auxin response factors

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