Phosphorylation of serine 392 in p53 is a common and integral event during p53 induction by diverse stimuli

Evaluation of an Actinomycin D/VX-680 aurora kinase inhibitor combination in p53-based cyclotherapy

“…Phosphorylation of p53 at serine 392 was initially observed after LDActD treatment, but not following Nutlin-3 treatment [17]. A more detailed analysis showed that phosphorylation at this site is also detectable in response to Nutlin-3 [25]. However, because of the nature of their p53-activation mechanisms, some differences in the speed at which p53 is induced by Nutlin-3 and ActD could be expected.…”

Mechanism-specific signatures for small-molecule p53 activators

“…8 This is also the case for actD at doses below 10 nM. 9,11 In contrast, at higher doses, actD has a substantial impact on Ser15.…”

The RAX/PACT-PKR stress response pathway promotes p53 sumoylation and activation, leading to G1arrest

“…Significantly, others have reported that p53 activation leading to G 1 arrest is impaired in pkr -/-cells, and that increased PKR expression/activity will promote p53 stability. 23,43 It has also been reported that PKR can phosphorylate p53 on serine 392, and that phosphorylation may inhibit the turnover of p53 leading to enhanced stability. 21,30 Findings here both support and extend this mechanism, Luciferase reporter assay.…”

“…An integral membrane GFP marker, Us9-GFP, was used to test for the positively transfected cells in FACS analysis. 43 Cells were transfected with lipofectamine 2000 with Us9-GFP and other plasmid as indicated. Twenty-four hours post transfection cells were treated with trypsin, fixed and stained by propidium iodide.…”