2009
DOI: 10.1038/ncb1995
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Phosphorylation of STIM1 underlies suppression of store-operated calcium entry during mitosis

Abstract: SUMMARY Store-operated Ca2+ entry (SOCE) and Ca2+ release-activated Ca2+ currents (Icrac) are strongly suppressed during mitosis, the only known physiological situation in which Ca2+ store depletion is uncoupled from the activation of Ca2+ influx. We found that the ER Ca2+ sensor, STIM1, failed to rearrange into near-plasma membrane puncta in mitotic cells, a critical step in the SOCE activation pathway. We also found that STIM1 from mitotic cells is recognized by the phosphospecific MPM-2 antibody, suggesting… Show more

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Cited by 162 publications
(141 citation statements)
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“…Several phosphorylated residues in STIM1 were found in response to epidermal growth factor stimulation in HeLa cells, but the more detailed physiological connection of these phosphorylations with STIM1 function in SOCE activation remains impalpable [14, 15]. Recent studies have revealed that SOCE is inhibited by phosphorylation of STIM1 in non-excitable cells [15, 47]. Some studies have demonstrated that the phosphorylation of STIM1 in ERK1/2 target serine residues is essential to fully trigger SOCE [14].…”
Section: Discussionmentioning
confidence: 99%
“…Several phosphorylated residues in STIM1 were found in response to epidermal growth factor stimulation in HeLa cells, but the more detailed physiological connection of these phosphorylations with STIM1 function in SOCE activation remains impalpable [14, 15]. Recent studies have revealed that SOCE is inhibited by phosphorylation of STIM1 in non-excitable cells [15, 47]. Some studies have demonstrated that the phosphorylation of STIM1 in ERK1/2 target serine residues is essential to fully trigger SOCE [14].…”
Section: Discussionmentioning
confidence: 99%
“…Fig. 2 (A and B) shows that STIM2 was diffusely distributed in proliferation (control) and during the first 30 min of differentiation with only a few clusters detectable (50). During the initiation of differentiation, however, STIM2 formed discrete clusters of progressively increasing size (Fig.…”
Section: Stim1 and Stim2 Form Discrete Clusters During Initiation Of mentioning
confidence: 99%
“…Putney and colleagues demonstrated the first example of phosphorylation mediated regulation of STIM1 during mitosis. 87 It was known that SOCE was suppressed during cell division, however, the molecular mechanism behind this event was not known. Smyth et al showed that S486 and S668 located within the serine/threonine-rich domain of STIM1 were phosphorylated during mitosis by cyclin dependent kinase 1 (CDK1) and these phosphorylations suppressed SOCE (Fig.…”
Section: Posttranslational Modification Of Orai1 and Stim1mentioning
confidence: 99%