2023
DOI: 10.7554/elife.82428
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Phosphorylation of tyrosine 90 in SH3 domain is a new regulatory switch controlling Src kinase

Abstract: The activation of Src kinase in cells is strictly controlled by intramolecular inhibitory interactions mediated by SH3 and SH2 domains. They impose structural constraints on the kinase domain holding it in a catalytically non‑permissive state. The transition between inactive and active conformation is known to be largely regulated by the phosphorylation state of key tyrosines 416 and 527. Here we identified that phosphorylation of tyrosine 90 reduces binding affinity of the SH3 domain to its interacting partne… Show more

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“…The spheroid invasion assay was performed following a previously published protocol. 63 Briefly, BLM cells were grown as spheroids using 3D Petri Dish (Microtissues). After 2 days of spheroid formation, 1.5 mg mL −1 collagen solution (rat tail, collagen type I) was prepared (1.5 mg mL −1 rat-tail collagen, 1× DMEM, 1% (v/v) FBS) and added at the bottom of a 96-well plate.…”
Section: Methodsmentioning
confidence: 99%
“…The spheroid invasion assay was performed following a previously published protocol. 63 Briefly, BLM cells were grown as spheroids using 3D Petri Dish (Microtissues). After 2 days of spheroid formation, 1.5 mg mL −1 collagen solution (rat tail, collagen type I) was prepared (1.5 mg mL −1 rat-tail collagen, 1× DMEM, 1% (v/v) FBS) and added at the bottom of a 96-well plate.…”
Section: Methodsmentioning
confidence: 99%