Phosphorylation-regulated HMGA1a-P53 interaction unveils the function of HMGA1a acidic tail phosphorylations via synthetic proteins

Wei, Tongyao; Liu, Heng; Chu, Binbin; Blasco, Pilar; Liu, Zheng; Tian, Ruijun; Li, David Xiang; Li, Xuechen

doi:10.1016/j.chembiol.2021.01.007

Cited by 12 publications

(20 citation statements)

References 59 publications

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“… Note: Coupling Fmoc-Ser(HPO 3 Bzl)-OH at Ser98, Ser101, and Ser102 using HATU/HOBt (4 equivalent) in DMF/NMP (1/1, v/v) as coupling reagent. CRITICAL: Coupling Fmoc-Gly(Hmb)-OH at Gly96 for 12 h. Fmoc-Gly(Hmb)-OH is commercially available, or refer to the synthetic route at Wei et al., 2021 . After coupling of the last Boc-Ser(tBu)-OH, treat resin with 10 mL 2% hydrazine in DMF (v/v) and shake for 30 min to liberate hydroxyl group on Hmb.…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… CRITICAL: Coupling Fmoc-Gly(Hmb)-OH at Gly96 for 12 h. Fmoc-Gly(Hmb)-OH is commercially available, or refer to the synthetic route at Wei et al., 2021 .…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…The synthesis involves C-to-N sequential Ser/Thr ligations. This figure is reprinted with permission from Wei et al., 2021 . (B) Effect of C-terminal amino acid on STL by a model study.…”

Section: Before You Begin ( Thompson and Muir 2019 )mentioning

confidence: 99%

“…The synthesis involves C-to-N sequential Ser/Thr ligations. This figure is reprinted with permission from Wei et al., 2021 .…”

Section: Before You Begin ( Thompson and Muir 2019 )mentioning

confidence: 99%

“…Here, a detailed protocol is introduced for chemical synthesis of HMGA1a protein with site-specific modifications via Ser/Thr ligation strategy, by which we can systematically study the function of the triple phosphorylation (3pSer) in the HMGA1a acidic tail. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021) .…”

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confidence: 99%

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Serine/threonine ligation-assisted chemical synthesis of HMGA1a protein with site-specific post-translational modifications

Wei

Liu

et al. 2021

STAR Protocols

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Summary Dissecting the function of proteins’ post-translational modifications (PTMs) is seriously hindered by the difficulty in obtaining the homogeneous protein with the PTMs of interest. Chemical protein synthesis offers a great potential to overcome this limitation. Here, a detailed protocol is introduced for chemical synthesis of HMGA1a protein with site-specific modifications via Ser/Thr ligation strategy, by which we can systematically study the function of the triple phosphorylation (3pSer) in the HMGA1a acidic tail. For complete details on the use and execution of this protocol, please refer to Wei et al. (2021) .

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Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“… CRITICAL: Coupling Fmoc-Gly(Hmb)-OH at Gly96 for 12 h. Fmoc-Gly(Hmb)-OH is commercially available, or refer to the synthetic route at Wei et al., 2021 .…”

Section: Step-by-step Methods Detailsmentioning

confidence: 99%

“…The synthesis involves C-to-N sequential Ser/Thr ligations. This figure is reprinted with permission from Wei et al., 2021 . (B) Effect of C-terminal amino acid on STL by a model study.…”

Section: Before You Begin ( Thompson and Muir 2019 )mentioning

confidence: 99%

“…The synthesis involves C-to-N sequential Ser/Thr ligations. This figure is reprinted with permission from Wei et al., 2021 .…”

Section: Before You Begin ( Thompson and Muir 2019 )mentioning

confidence: 99%

mentioning

confidence: 99%

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Serine/threonine ligation-assisted chemical synthesis of HMGA1a protein with site-specific post-translational modifications

Wei

Liu

et al. 2021

STAR Protocols

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Selective Peptide Cysteine Manipulation on Demand and Difficult Protein Chemical Synthesis Enabled by Controllable Acidolysis of N,S‐Benzylidene Thioacetals

Wu,

Sun,

2024

Angewandte Chemie

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Although solid‐phase peptide synthesis combining with chemical ligation provides a way to build up customized polypeptides in general, many targets are still presenting challenges for the conventional synthetic process, such as hydrophobic proteins. New methods and strategies are still required to overcome these obstacles. In this study, kinetic studies of Cys/Pen ligation and its acidolysis were performed, from which the fast acidolysis of substituted N,S‐Benzylidene Thioacetals (NBTs) was discovered. The study demonstrates the potential of NBTs as a promising Cys switchable protection, facilitating the chemical synthesis of peptides and proteins by efficiently disrupting peptide aggregation. The compatibility of NBTs with other commonly adopted Cys protecting groups and their applications in sequential disulfide bond formation were also investigated. The first chemical synthesis of the native human programmed death ligand 1 immunoglobulin V‐like (PD‐L1 IgV) domain was achieved using the NBT strategy, showcasing its potentials in difficult protein synthesis

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Selective Peptide Cysteine Manipulation on Demand and Difficult Protein Chemical Synthesis Enabled by Controllable Acidolysis of N,S‐Benzylidene Thioacetals

Wu,

Sun,

2024

Angew Chem Int Ed

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Phosphorylation-regulated HMGA1a-P53 interaction unveils the function of HMGA1a acidic tail phosphorylations via synthetic proteins

Cited by 12 publications

References 59 publications

Serine/threonine ligation-assisted chemical synthesis of HMGA1a protein with site-specific post-translational modifications

Serine/threonine ligation-assisted chemical synthesis of HMGA1a protein with site-specific post-translational modifications

Selective Peptide Cysteine Manipulation on Demand and Difficult Protein Chemical Synthesis Enabled by Controllable Acidolysis of N,S‐Benzylidene Thioacetals

Selective Peptide Cysteine Manipulation on Demand and Difficult Protein Chemical Synthesis Enabled by Controllable Acidolysis of N,S‐Benzylidene Thioacetals

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