Photic control of nitric oxide synthase activity in golden hamster retina

Llomovatte, Diego Weinberg; Lacoste, Francisco Firpo; Zotter, Carolina; Sarmiento, María I. Keller; Rosenstein, Ruth E.

doi:10.1097/00001756-199712010-00021

Cited by 10 publications

(12 citation statements)

References 6 publications

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“…In a previous report, we showed that NOS activity is regulated by the photic information (Llomovatte et al . 1997) in a similar way to that showed herein for l ‐arginine uptake.…”

Section: Discussionmentioning

confidence: 83%

“…A necessary (although not suf®cient) condition to prove this idea would be to demonstrate changes in NOS activity in response to photic stimulus. Indeed, in a previous report, we demonstrated that retinal NOS activity in the hamster is regulated by light signals (Llomovatte et al 1997), being higher during the night than during the day. In addition, we showed that cGMP levels in the hamster retina are signi®cantly higher during the dark than during the light phase (Faillace et al 1995), in a similar manner than NOS activity.…”

mentioning

confidence: 65%

“…A necessary (although not sufficient) condition to prove this idea would be to demonstrate changes in NOS activity in response to photic stimulus. Indeed, in a previous report, we demonstrated that retinal NOS activity in the hamster is regulated by light signals (Llomovatte et al . 1997), being higher during the night than during the day.…”

mentioning

confidence: 75%

“…Therefore, it seems likely that to induce the activation of NOS, an obligatory influx of l ‐arginine is required. In the golden hamster, NOS activity was higher at the same time point in which the influx of l ‐arginine increased (Llomovatte et al . 1997).…”

Section: Discussionmentioning

confidence: 99%

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Photic regulation of l‐arginine uptake in the golden hamster retina

Sáenz¹,

Cymeryng²,

Nichilo³

et al. 2002

Journal of Neurochemistry

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One of the limiting steps in the regulation of nitric oxide (NO) synthesis is the availability of its precursor, L-arginine, which depends on the presence of a speci®c uptake system. A characterization of the L-arginine uptake mechanism in the golden hamster retina was performed. This mechanism was stereospeci®c, saturable, and monophasic, with an apparent K m of 56.1 2.0 lM and a maximum velocity of 36.0 2.8 pmol/mg prot/min. The basic amino acids L-lysine and L-ornithine but not D-arginine or the nitric oxide synthase inhibitors, N x -nitro-L-arginine methyl ester and N x -nitro-Larginine impaired L-arginine in¯ux. Preincubation with L-lysine for 1 h prior to the transport assay signi®cantly stimulated L-arginine uptake. Saturation studies of L-arginine uptake performed at 12.00 and 24.00 h indicated a higher value of V max at midnight than at midday. When the hamsters were placed under constant darkness or constant light for 48 h and killed at equivalent time points, representing subjective day and subjective night, the differences in L-arginine in¯ux disappeared. Semiquantitative RT-PCR analysis showed that the levels of mRNAs for both CAT-1 and CAT-2B were signi®-cantly higher at midnight than at midday. L-Arginine signi®-cantly increased cGMP accumulation in a time-dependent manner, with maximal effects during the night. Based on these results, it might be presumed that hamster retinal L-arginine uptake is regulated by the photic stimulus.

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“…In a previous report, we showed that NOS activity is regulated by the photic information (Llomovatte et al . 1997) in a similar way to that showed herein for l ‐arginine uptake.…”

Section: Discussionmentioning

confidence: 83%

mentioning

confidence: 65%

mentioning

confidence: 75%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Photic regulation of l‐arginine uptake in the golden hamster retina

Sáenz¹,

Cymeryng²,

Nichilo³

et al. 2002

Journal of Neurochemistry

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“…), and in the mammalian retina, NOS activity oscillates over the course of a day (Llomovatte et al . ; Zhang et al . ), we thereby investigated whether NO elicited a circadian phase‐dependent modulation of L‐VGCCs in avian cone photoreceptors.…”

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confidence: 99%

Circadian phase‐dependent effect of nitric oxide on L‐type voltage‐gated calcium channels in avian cone photoreceptors

Shi

Huang

et al. 2013

Journal of Neurochemistry

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Nitric oxide (NO) plays an important role in phase-shifting of circadian neuronal activities in the suprachiasmatic nucleus and circadian behavior activity rhythms. In the retina, NO production is increased in a light-dependent manner. While endogenous circadian oscillators in retinal photoreceptors regulate their physiological states, it is not clear whether NO also participates in the circadian regulation of photoreceptors. In the present study, we demonstrate that NO is involved in the circadian phase-dependent regulation of L-type voltage-gated calcium channels (L-VGCCs). In chick cone photoreceptors, the L-VGCCα1 subunit expression and the maximal L-VGCC currents are higher at night, and both Ras-MAPK (mitogen-activated protein kinase)-Erk (extracellular-signal-regulated kinase) and Ras-phosphatidylinositol 3 kinase (PI3K)-protein kinase B (Akt) are part of the circadian output pathways regulating L-VGCCs. The NO-cGMP-protein kinase G (PKG) pathway decreases L-VGCCα1 subunit expression and L-VGCC currents at night, but not during the day, and exogenous NO donor or cGMP decreases the phosphorylation of Erk and Akt at night. The protein expression of neural NO synthase (nNOS) is also under circadian control, with both nNOS and NO production being higher during the day. Taken together, NO/cGMP/PKG signaling is involved as part of the circadian output pathway to regulate L-VGCCs in cone photoreceptors.

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Postnatal development of nicotinamide adenine dinucleotide phosphate‐diaphorase‐positive neurons in the retina of the golden hamster

Tay

Diao

Xiao

et al. 2002

J of Comparative Neurology

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The histochemical method was used to investigate the postnatal development of nicotinamide adenine dinucleotide phosphate-diaphorase (NADPH-d) -positive neurons in retinas of the golden hamster. NADPH-d-positive neurons were discernible in the retina at postnatal day (P)1. From P4 onward to adulthood, when the retina acquired its laminated characteristics, NADPH-d- positive neurons were observed in the inner nuclear layer (INL) and the ganglion cell layer (GCL). Results showed that NADPH-d-positive neurons in INL and GCL followed different time courses and patterns in their development. NADPH-d-positive neurons in INL underwent a sharp increase from P4 to P8 (3.6-fold), followed by a decrease to 46% of the maximum at P12. This value was maintained relatively constant to the adult level. The mean diameters of NADPH-d-positive neurons in INL, which were smaller than those in the GCL for all ages, increased from P8 to P12 and from P20 to adulthood. As for neurons in the GCL, the increase in cell number was not so apparent for the earlier postnatal days until P20; thereafter, an obvious increase to the adult level was observed. The mean diameters of the NADPH-d-positive cell bodies in the GCL increased with age, except for P16-P20, during which time there was a slight and insignificant decrease. The tendency of changes in cell density was basically similar to that of the total number for both the INL and the GCL. Between P12 and P20, the density distribution map of the NADPH-d-positive neurons underwent dramatic changes: The highest density shifted from the upper central retina at the earlier postnatal days to the lower central retina in the adult. The two waves of increase in NADPH-d-positive neurons coincide with the process of axonal elongation and synaptogenesis and the acquisition of visual function and experience. It is suggested that these NADPH-d-positive neurons are related to these two developmental events.

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Photic control of nitric oxide synthase activity in golden hamster retina

Cited by 10 publications

References 6 publications

Photic regulation of l‐arginine uptake in the golden hamster retina

Photic regulation of l‐arginine uptake in the golden hamster retina

Circadian phase‐dependent effect of nitric oxide on L‐type voltage‐gated calcium channels in avian cone photoreceptors

Postnatal development of nicotinamide adenine dinucleotide phosphate‐diaphorase‐positive neurons in the retina of the golden hamster

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