Photo-Cleavable Peptide-Poly(Ethylene Glycol) Conjugate Surfaces for Light-Guided Control of Cell Adhesion

Abstract

Photo-responsive cell attachment surfaces can simplify patterning and recovery of cells in microdevices for medicinal and pharmaceutical research. We developed a photo-responsive surface for controlling the attachment and release of adherent cells on a substrate under light-guidance. The surface comprises a poly(ethylene glycol) (PEG)-based photocleavable material that can conjugate with cell-adhesive peptides. Surface-bound peptides were released by photocleavage in the light-exposed region, where the cell attachment was subsequently suppressed by the exposed PEG. Simultaneously, cells selectively adhered to the peptide surface at the unexposed microscale region. After culture, the adhered and spread cells were released by exposure to a light with nontoxic dose level. Thus, the present surface can easily create both cell-adhesive and non-cell-adhesive regions on the substrate by single irradiation of the light pattern, and the adhered cells were selectively released from the light-exposed region on the cell micropattern without damage. This study shows that the photo-responsive surface can serve as a facile platform for the remote-control of patterning and recovery of adherent cells in microdevices.

Novel Quick Cell Patterning Using Light-Responsive Gas-Generating Polymer and Fluorescence Microscope

“…For example, when culturing adhesive cells, an ECM such as fibronectin or collagen must be used, but simultaneously using the ECM and chemical coated substances for cell patterning is extremely difficult because they overlap on the substrate and cancel each other's effect. Previous research tried to accurate cell patterning with a coating multilayer that consists of two kinds of chemical materials for cell adsorbing and releasing [31,32]. However, it is extremely difficult to simultaneously use tissue-derived biomaterial (e.g., collagen, fibronectin, type of ECM) for cell adhesion and culture.…”

Editorial for the Special Issue on Micro and Nano Devices for Cell Analysis

“…Furthermore, cell analysis devices combined with chemical and biochemical materials are also reported. Yamaguchi et al [ 10 ] developed a photo-responsive surface for controlling the attachment and release of adherent cells on a substrate under light guidance. The surface comprises a poly (ethylene glycol) (PEG)-based photocleavable material that can conjugate with cell-adhesive peptides (arginine-glycine-aspartic acid (RGD) motif).…”