Photo controlled release of nitric oxide (NO) from amphiphilic and nanoscale vesicles based ruthenium nitrosyl complex: NO release and cytotoxicity studies

Sharma, Nancy; Arjunan, Porkizhi; Marepally, Srujan; Jain, Nimisha; Naziruddin, Abbas Raja; Ghosh, Amrita; Mariappan, C.R.

doi:10.1016/j.jphotochem.2021.113703

Cited by 15 publications

(8 citation statements)

References 45 publications

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“…However, the complex L.Ru-NO is not water-soluble, which is a trouble for biological study and application. As observed in our earlier studies, the bio-incompatibility of the amphiphilic metal nitrosyl complex could be improved by incorporating the Ru-NO donor in self-assembling soft materials such as liposomes . Liposomes are biocompatible and are used for site-specific delivery without showing biological activity.…”

Section: Resultsmentioning

confidence: 58%

“…As observed in our earlier studies, the bio-incompatibility of the amphiphilic metal nitrosyl complex could be improved by incorporating the Ru-NO donor in self-assembling soft materials such as liposomes. 38 Liposomes are biocompatible and are used for site-specific delivery without showing biological activity. Therefore, we incorporated our amphiphilic ruthenium complex L.Ru-NO with the commercial phospholipids to form the nanoscale vesicles.…”

Section: Under Reflux Condition (Schemes 1 and S1)mentioning

confidence: 99%

“…Ligand L was synthesized according to the procedure reported in our previous work 38 (see Figures S1 and S2). To prepare L.Ru-NO, ligand L (0.2595 mmol, 150 mg) was dissolved in 30 mL of EtOH:H 2 O (3:1) mixture under reflux condition.…”

Section: Synthesis Of [Ru II (Cl) 2 No(l)](pf 6 ) (Lru-no)mentioning

confidence: 99%

“…Vesicles. Unilamellar vesicles were prepared by using 5 mol % of the L.Ru-NO complex and lipids such as DOPC, DSPC, DPPC, and DMPC to prepare four different sets of vesicles by the thin-film hydration method 38 using extruder purchased from Avanti Polar Lipids. For each lipid, 0.5 μmol solution of L.Ru-NO in CHCl 3 was added to a 10 μmol solution of the respective lipid (DOPC/DSPC/DPPC/DMPC) in chloroform.…”

Section: Preparation Of Nanoscalementioning

confidence: 99%

“…37 Incorporation of the Ru-NO complex on nanoscale materials is rare. 38,39 The role of membrane fluidity in vesicular systems was investigated in a few examples such as photocatalytic hydrogen evolution 40 and chemical sensing. 41,42 However, membrane fluidity control on NO delivery from Ru-NO complex-incorporated vesicular systems is not known.…”

Section: Introductionmentioning

confidence: 99%

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Regulation of Nitric Oxide (NO) Release by Membrane Fluidity in Ruthenium Nitrosyl Complex-Embedded Phospholipid Vesicles

et al. 2022

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Incorporating water-insoluble nitric oxide (NO)-releasing molecules into biocompatible vesicles may allow for the tunable control of NO release on a specific target site. In vesicles, membrane fluidity plays an important role and influences the final therapeutic efficiency of drugs loaded into the vesicles. Hence, we aimed to investigate the effect of lipid fluidity on the NO release behavior of the photo-controllable ruthenium nitrosyl (Ru-NO) complex. In this regard, a new photoactive ruthenium nitrosyl complex (L.Ru-NO) with amphiphilic terpyridine ligand was synthesized and characterized in detail. L.Ru-NO was incorporated with commercial phospholipids to form nanoscale vesicles L.Ru-NO@Lip. The photoactive {Ru-NO}6 type complex released NO in the organic solvent CH3CN and aqueous liposome solution by irradiating under low-intensity blue light (λ = 410 nm, 3 W). To demonstrate the effect of lipid structure and fluidity on NO release, four different liposome systems L.Ru-NO@Lip1–4 were prepared by using phospholipids such as DOPC, DSPC, DPPC, and DMPC having different chain lengths and saturation. The NO-releasing abilities of these liposomes in aqueous medium were studied by UV–vis spectrum, colorimetric Greiss, and fluorescent DAF assay. The results show that the rate of NO release could be easily tuned by varying the lipid fluidity. The effect of temperature and pH on NO release was also studied. Further, the complex L.Ru-NO and liposomes L.Ru-NO@Lip1 were assayed as an antibacterial agent against the strains of bacteria Escherichia coli and Staphylococcus aureus.

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Section: Resultsmentioning

confidence: 58%

Section: Under Reflux Condition (Schemes 1 and S1)mentioning

confidence: 99%

Section: Synthesis Of [Ru II (Cl) 2 No(l)](pf 6 ) (Lru-no)mentioning

confidence: 99%

Section: Preparation Of Nanoscalementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Regulation of Nitric Oxide (NO) Release by Membrane Fluidity in Ruthenium Nitrosyl Complex-Embedded Phospholipid Vesicles

et al. 2022

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Bimetallic Ruthenium Nitrosyl Complexes with Enhanced Two‐Photon Absorption Properties for Nitric Oxide Delivery

Juarez-Martinez

Labra‐Vázquez

Enríquez-Cabrera

et al. 2022

Chemistry A European J

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One monometallic and three bimetallic ruthenium nitrosyl (RuNO) complexes are presented and fully characterized in reference to a parent monometallic complex of formula [FTRu(bpy)(NO)] 3 + , where FT is a fluorenyl-substituted terpyridine ligand, and bpy the 2,2'-bipyridine. These new complexes are built with the new ligands FFT, TFT, TFFT, and TF-CC-TF (where an alkyne C�C group is inserted between two fluorenes). The crystal structures of the bis-RuNO 2 and bis-RuNO complexes built from the TFT ligand are presented. The evolution of the spectroscopic features (intensities and energies) along the series, at one-photon absorption (OPA) correlates well with the TD-DFT computations. A spectacular effect is observed at two-photon absorption (TPA) with a large enhancement of the molecular cross-section (σ TPA ), in the bimetallic species. In the best case, σ TPA is equal to 1523 � 98 GM at 700 nm, in the therapeutic window of transparency of biological tissues. All compounds are capable of releasing NO * under irradiation, which leads to promising applications in TPA-based drug delivery.

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Gas Therapy: Generating, Delivery, and Biomedical Applications

Ghaffari‐Bohlouli,

Jafari,

Okoro

et al. 2024

Small Methods

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Oxygen (O2), nitric oxide (NO), carbon monoxide (CO), hydrogen sulfide (H2S), and hydrogen (H2) with direct effects, and carbon dioxide (CO2) with complementary effects on the condition of various diseases are known as therapeutic gases. The targeted delivery and in situ generation of these therapeutic gases with controllable release at the site of disease has attracted attention to avoid the risk of gas poisoning and improve their performance in treating various diseases such as cancer therapy, cardiovascular therapy, bone tissue engineering, and wound healing. Stimuli‐responsive gas‐generating sources and delivery systems based on biomaterials that enable on‐demand and controllable release are promising approaches for precise gas therapy. This work highlights current advances in the design and development of new approaches and systems to generate and deliver therapeutic gases at the site of disease with on‐demand release behavior. The performance of the delivered gases in various biomedical applications is then discussed.

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Photo controlled release of nitric oxide (NO) from amphiphilic and nanoscale vesicles based ruthenium nitrosyl complex: NO release and cytotoxicity studies

Cited by 15 publications

References 45 publications

Regulation of Nitric Oxide (NO) Release by Membrane Fluidity in Ruthenium Nitrosyl Complex-Embedded Phospholipid Vesicles

Regulation of Nitric Oxide (NO) Release by Membrane Fluidity in Ruthenium Nitrosyl Complex-Embedded Phospholipid Vesicles

Bimetallic Ruthenium Nitrosyl Complexes with Enhanced Two‐Photon Absorption Properties for Nitric Oxide Delivery

Gas Therapy: Generating, Delivery, and Biomedical Applications

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