Photo-enhancement of macrophage phagocytic activity via Rac1-mediated signaling pathway: Implications for bacterial infection

Lu, Cuixia; Fan, Zhijin; Xing, Da

doi:10.1016/j.biocel.2016.06.010

Cited by 15 publications

(12 citation statements)

References 53 publications

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“…3 E). The effects of laser irradiation on the production of reactive oxygen species in cells have been discussed in previous studies [ 46 ], but the phenomenon is not significant. Thus, ICG-CpG@MOF is the main reason for the increase of reactive oxygen species after laser irradiation.…”

Section: Resultsmentioning

confidence: 99%

“…The mouse breast cancer cell line 4T1 cells were obtained from the Cell bank of the Animal Experiment Center of Sun Yat-sen University. The peritoneal macrophage is acquired by the peritoneal lavatory as we did before [ 46 ]. Spleen lymphocytes were isolated as previously reported [ 49 ].…”

Section: Methodsmentioning

confidence: 99%

“…Lambda 900 spectrophotometer (PerkinElmer, USA) was used to measure the UV–Vis absorption spectra of ICG-CpG@MOF. FL-8500 fluorescence spectrophotometer (PerkinElmer, USA) was used to record the fluorescence excitation and emission spectra of ICG-CpG@MOF, as we reported earlier [ 46 ]. A Nikon confocal laser scanning microscope was used to observe the internalization of fluorescently labeled MOF with a slight change from previous article [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

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Reversing cold tumors to hot: An immunoadjuvant-functionalized metal-organic framework for multimodal imaging-guided synergistic photo-immunotherapy

Fan

Liu

Xue

et al. 2021

Bioactive Materials

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135

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Immunotherapy assays using immunoadjuvants and tumor antigens could greatly increase the survival rates of patients with malignant tumors. As effective carriers, metal-organic frameworks (MOFs) have been widely utilized in cancer therapy due to their remarkable histocompatibility and low toxicity. Herein, we constructed a multimodal imaging-guided synergistic cancer photoimmunotherapy by employing a specific MOF (MIL101-NH 2 ) as the core carrier; the MOF was dual-dressed with photoacoustic and fluorescent signal donors (indocyanine green, ICG) and immune adjuvants (cytosine-phosphate-guanine sequence, CpG) and named ICG-CpG@MOF. This nanocarrier could passively target the tumor site through the EPR effect and achieve multimodal imaging (fluorescence, photoacoustic, photothermal and magnetic resonance imaging) of the tumor. Synergistic cancer photoimmunotherapy was achieved via simultaneous photodynamic and photothermal methods with 808 nm laser irradiation. ICG-CpG@MOF achieved the GSH-controlled release of immunoadjuvant into the tumor microenvironment. Furthermore, the released tumor-associated antigen along with CpG could induce the transformation of tumor cells from cold to hot by activating the immune system, which significantly enhanced tumor cytotoxicity and achieved high cure rates with minimal side-effects. This strategy utilizing multimodal imaging and synergistic cancer photoimmunotherapy provides a promising approach for the diagnosis and treatment of cancer.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Reversing cold tumors to hot: An immunoadjuvant-functionalized metal-organic framework for multimodal imaging-guided synergistic photo-immunotherapy

Fan

Liu

Xue

et al. 2021

Bioactive Materials

Self Cite

135

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“…LSM : According to a published article, fluorescence imaging was observed by LSM (ZEISS LSM 780, Germany) using a 40 × oil objective lens. DIO fluorescence was excited using a 488 nm Ar ion laser, and the fluorescence emission was detected through a 500–530 nm bandpass filter.…”

Section: Methodsmentioning

confidence: 99%

Functionalized DNA Enables Programming Exosomes/Vesicles for Tumor Imaging and Therapy

Fan

Xiao

Lin

et al. 2019

Small

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Exosomes serve as significant information carriers that regulate important physiological and pathological processes. Herein, functionalized DNA is engineered to be a hinge that anchors quantum dots (QDs) onto the surface of exosomes, realizing a moderate and biocompatible labeling strategy. The QDs‐labeled exosomes (exosome–DNA–QDs complex) can be swiftly engulfed by tumor cells, indicating that exosome–DNA–QDs can be applied as a specific agent for tumor labeling. Furthermore, the engineered artificial vesicles of M1 macrophages (M1mv) are constructed via a pneumatic liposome extruder. The results reveal that the individual M1mv can kill tumor cells and realize desirable biological treatment. To reinforce the antitumor efficacy of M1mv and the specificity of drug release, a target‐triggered drug delivery system is constructed to realize a specific microRNA‐responded delivery system for visual therapy of tumors. These strategies facilitate moderate labeling and functionalization of exosomes/vesicles and construct artificial drug‐delivery vesicles that simultaneously possess biological treatment and chemotherapy functions, and thus have the potential to serve as a new paradigm for tumor labeling and therapy.

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“…underlying anti-inflammatory effects) responsive to ROS signaling pathways [eg. 17,18,[37][38][39]. This is also true for resolution of chronic and acute pain, for which PEMF therapy has been proven effective, and which has the great advantage that there are no potential negative side effects as caused by pharmaceutical remedies [40].…”

Section: Plos Onementioning

confidence: 98%

HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

Pooam

Jourdan²,

Esawi

et al. 2020

PLoS ONE

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PEMF (Pulsed Electromagnetic Field) stimulation has been used for therapeutic purposes for over 50 years including in the treatment of memory loss, depression, alleviation of pain, bone and wound healing, and treatment of certain cancers. However, the underlying cellular mechanisms mediating these effects have remained poorly understood. In particular, because magnetic field pulses will induce electric currents in the stimulated tissue, it is unclear whether the observed effects are due to the magnetic or electric component of the stimulation. Recently, it has been shown that PEMFs stimulate the formation of ROS (reactive oxygen species) in human cell cultures by a mechanism that requires cryptochrome, a putative magnetosensor. Here we show by qPCR analysis of ROS-regulated gene expression that simply removing cell cultures from the Earth’s geomagnetic field by placing them in a Low-Level Field condition induces similar effects on ROS signaling as does exposure of cells to PEMF. This effect can be explained by the so-called Radical Pair mechanism, which provides a quantum physical means by which the rates and product yields (e.g. ROS) of biochemical redox reactions may be modulated by magnetic fields. Since transient cancelling of the Earth’s magnetic field can in principle be achieved by PEMF exposure, we propose that the therapeutic effects of PEMFs may be explained by the ensuing modulation of ROS synthesis. Our results could lead to significant improvements in the design and therapeutic applications of PEMF devices.

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Photo-enhancement of macrophage phagocytic activity via Rac1-mediated signaling pathway: Implications for bacterial infection

Cited by 15 publications

References 53 publications

Reversing cold tumors to hot: An immunoadjuvant-functionalized metal-organic framework for multimodal imaging-guided synergistic photo-immunotherapy

Reversing cold tumors to hot: An immunoadjuvant-functionalized metal-organic framework for multimodal imaging-guided synergistic photo-immunotherapy

Functionalized DNA Enables Programming Exosomes/Vesicles for Tumor Imaging and Therapy

HEK293 cell response to static magnetic fields via the radical pair mechanism may explain therapeutic effects of pulsed electromagnetic fields

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