Photo‐induced hydrophilicity enhances initial cell behavior and early bone apposition

Saeki, Takashi; Jimbo, Ryo; Baba, Koumei; Shibata, Yasuaki; Ikeda, Tohru; Atsuta, Mitsuru

doi:10.1111/j.1600-0501.2007.01509.x

Cited by 152 publications

(118 citation statements)

References 29 publications

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“…Since the method of rapid colorimetric MTT is based on metabolic cell activity, the present study results can be more reliable than those reported by Sawase et al (2008), where counting the number of adherent cells was performed by laser microscopy and specialized software; on the other hand Onuki et al (2010) detached with trypsin adherent cells and cell counting was performed with hemocytometer and finally Koseki et al (2009) isolated cells from the surface with trypsin and ultrasonic waves, those methods previously reported are ineffective for correct estimate of the number of viable cells. However, the MTT method has a limitation, minimum cells still remain on the surface, underestimation due to lack of contact time MTT reagent with cells, therefore, in future research an increase in the incubation time of the reagent with cells is recommended.…”

Section: Discussioncontrasting

confidence: 55%

Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion

Ornelas-Ponce

Amezcua-Romero

Acosta-Torres

et al. 2016

Int. J. Odontostomat.

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ORNELAS-PONCE, A.; AMEZCUA-ROMERO, J.; ACOSTA-TORRES, L. S. & GARCIA-CONTRERAS, R. Effects of TiO 2NPs coating-titanium for fibroblast adhesion. Int. J. Odontostomat., 10(2):237-242, 2016. ABSTRACT:The objective of this study was to determine the effects of coating nanoparticles of titanium dioxide (TiO 2 NPs) and irradiation -UV on plates of titanium (Ti) for the adhesion and proliferation of human gingival fibroblasts (HGF). A total of 15 Ti plates were divided into three groups (n = 5); (i) control Ti, (ii) experimental: Ti+TiO 2 NPs, (iii) experimental: Ti+TiO 2 NPs+UV. The plates were analyzed with atomic force microscopy (AFM) and the roughness (Ra and Rmax) was determined. UV irradiation was performed for 20 min. HGF were subcultured in DMEM+10 % fetal bovine serum (FBS) at 37 °C with 5 % CO 2 . 2x106 cells/mL were inoculated on the plates and incubated for 1 h and washed with phosphate buffer saline (PBS). In the case of cell proliferation, cells were incubated for further 24 h more. Cell viability was determined with the MTT method, the formazan was dissolved with dimethylsulfoxide (DMSO) and analyzed at 540 nm. Experiments were performed of three independent experiments and data were analyzed by Kruskall-Wallis and multiple comparison of MannWhitney test. The surface topography of samples corresponded as follow: Ti (Ra= 0,492 µm y Rms= 0.640 µm), Ti+NPs TiO 2 , (Ra= 0.55 µm y Rms= 0.714 µm), respectively. The coating with TiO 2 NPs significantly (p <0.05) increased the adhesion and proliferation of HGF compared with the group. The modification of Ti plates by coated with TiO 2 NPs significantly increased adhesion and proliferation of HGF with the formation of a hydrophilic surface which favors the humectancy. This treatment may be reported here convenient to accelerate osseointegration of dental implants based titanium.

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Section: Discussioncontrasting

confidence: 55%

Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion

Ornelas-Ponce

Amezcua-Romero

Acosta-Torres

et al. 2016

Int. J. Odontostomat.

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“…Sawase et al 30) found that Ti surfaces modified to attain photocatalytic hydrophilicity led to a significant increase in the attachment, spreading and proliferation of C2C12 cells, and such effects seemed to accelerate the early bone apposition to the implant surface. The present results obtained by culturing C2C12 directly on the cured materials indicate the possible advantage of Bis-GMA/TEGDMA-based composite resins or 4-META/ MMA-based resins to induce better biological responses in tissue regeneration.…”

Section: Discussionmentioning

confidence: 99%

Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

et al. 2010

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This study investigated the proliferation and differentiation potential of pluripotent mesenchymal cells on three resin-based restoratives using a typical pluripotent mesenchymal precursor cell line, C2C12. C2C12 cells were cultured for 3-21 days on cured specimens of a Bis-GMA/TEGDMA-based composite resin (APX; Clearfil AP-X), a 4-META/MMA-based resin cement (SB; Superbond C&B) or a HEMA-containing resin modified glass-ionomer (LC; Fuji Ionomer Type II LC). To examine the influences on differentiation potential, alkaline phosphatase (ALP) activity of the cells cultured on each material was determined. On APX and SB, cells adhered and proliferated well, and no significant influences on ALP activity were observed. In contrast, poor cell proliferation and significant suppression of ALP activity were observed for cells cultured on LC, similar to those cultured on a zinc oxide EBA cement used as a control material. Bis-GMA/TEGDMA-based composite resin and 4-META/MMA-based resin exhibited better biocompatibility for C2C12 cells than HEMA-containing resin modified glass-ionomer, suggesting a potential advantage of the former two resins to show smaller influences on regeneration of periapical or periodontal tissue.

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“…Since the surface roughness of CFRC was not significantly changed by the coating with the carbon-titanium layer, the beneficial effects of this coating on cell colonization could be explained by spontaneous oxidation of Ti and C in the cell culture system, including the formation of titanium oxides and oxygen-containing chemical functional groups. These features are known to enhance cell adhesion and growth (He et al, 2008;Sawase et al, 2008) and attract phosphate and calcium ions (Toworfe et al, 2006;Rakngarm et al, 2008), which facilitate bone tissue formation and bonding between the artificial implant and bone. Interestingly, the improvement of cell colonization by the C:Ti film was more pronounced in VSMC than in MG 63 cells, which was probably due to a different sensitivity of low-passaged cells and cell line to the physicochemical properties of the material surface.…”

Section: Amorphous Carbonmentioning

confidence: 99%

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

Bačáková¹,

Grausova²,

Vacı́k³

et al. 2011

Advances in Diverse Industrial Applications of Nanocomposites

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Photo‐induced hydrophilicity enhances initial cell behavior and early bone apposition

Abstract: The photo-induced hydrophilic surface used in the current study improves the initial cell reactions and enhances early bone apposition to the implant.

Cited by 152 publications

References 29 publications

Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion

Effects of TiO2 NPs Coating-Titanium for Fibroblast Adhesion

Proliferation and differentiation potential of pluripotent mesenchymal precursor C2C12 cells on resin-based restorative materials

Nanocomposite and Nanostructured Carbon-based Films as Growth Substrates for Bone Cells

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