Photo-Onycholysis Induced by 8-Methoxypsoralen

Zala, L; Omar, Amer; Krebs, A

doi:10.1159/000251067

Cited by 38 publications

(11 citation statements)

References 13 publications

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“…It appears, therefore, that most of the epidermal cells retain their mitotic ability despite the PUVA insult, and that they replicate with a distorted mitotic mechanism evolving into multinucleate cells. These results are in accordance with the histological findings of the photo-onycholytic nail bed which had revealed the highest number of multinucleate cells [2] as compared with the uninvolved skin of the PUVA-treated psoriatic patients [1], and implicate a temporal factor, namely, that cells in a tissue with a slow turnover would have ample time to germinate into multinucleate cells.…”

Section: Multinucleate Epidermal Cellssupporting

confidence: 89%

“…Histological sections prepared from biopsies of the uninvolved skin of PUVA-treated psoriatic patients and also the nail bed of patients who had contracted photo-onycholysis due to 8-MOP and sunlight irradiation, like wise revealed the presence of multinucleate epidermal cells and multinucleate fibroblasts [1,2]. These findings disclosed that PUVA treatment virtually had the same effect on the cells in vivo as in vitro.…”

Section: Introductionmentioning

confidence: 74%

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Polyploidization and Hemiploidization Induced by PUVA in vivo

Omar¹,

Wiesmann²,

Krebs³

1979

Dermatology

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To obtain additional information on the in vivo injury induced by PUVA treatment, primary cultures were initiated from biopsies of the uninvolved skin of 15 PUVA-treated psoriatic patients, and the cells generated were analysed for morphological and chromosomal modifications. Three biopsies were obtained from each patient, the first one before commencing PUVA therapy (control) and the other two during the course of therapy. It was found that PUVA treatment has diverse effects on the mitotic activity, mitotic mechanisms and chromosomes of the cutaneous cells: (1) an inhibition of cell proliferation during the early stages of therapy followed by a reversion to normal proliferation as the PUVA treatments were continued; the production of (2) cells with more than one nucleus, (3) macrocells, (4) cells with micronuclei, (5) polyploid cells, (6) haploid cells, (7) cells with 13 chromosomes denoting a twofold reduction in the normal number of metaphase chromosomes, (8) end-associations of the chromosomes as in the pachytene phase of meiosis (9) diplotene chromosomes, and (10) chromosomal translocations.

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Section: Multinucleate Epidermal Cellssupporting

confidence: 89%

Section: Introductionmentioning

confidence: 74%

Polyploidization and Hemiploidization Induced by PUVA in vivo

Omar¹,

Wiesmann²,

Krebs³

1979

Dermatology

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“…It appears that the multinucleate cells undergo a similar fate since these cells are also detected in the granular cell layers. Furthermore, the production of the multinucleate cells seem to be dependent on the turnover rate of the tissue because they are readily detected in the onycholytic nail bed [18] and in the uninvolved skin of the PUVA-treated psoriatic patients.…”

Section: Discussionmentioning

confidence: 99%

“…One biopsy was taken immediately after treatment and the other one 24 h later. Light-and electron-microscopic preparations were done as described previously [17,18], …”

Section: Light and Electron Microscopymentioning

confidence: 99%

Induction of Multinucleate Cells by 8-MOP and UV Treatment in vitro and in vivo

Omar¹,

Wiesmann²,

Krebs³

1977

Dermatology

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Fibroblast cells were treated with a single dose of 8-methoxypsoralen (8-MOP) and long-wave UV irradiation in vitro and the cytotoxic effects of the treatment followed up for 5 passages. During the first passage, there was an inhibition in cell proliferation, the induction of chromosomal aberrations and the production of macrocells as well as cells with more than one nucleus. In the subsequent passages, the proliferation rate of the cells gradually normalized but there was a concomitant increase in chromosomal aberrations and in the number of macrocells and multinucleate cells. Specimens, which were taken from the uninvolved skin of psoriatic patients who had been treated between 20 and 40 times with an oral dose of 8-MOP and black light irradiation (PUVA) also revealed the presence of binucleate, tetranucleate and multinucleate epidermal cells as well as binucleate and tetranucleate dermal fibroblasts.

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“…It is unlikely that photochemotherapy was responsible for the onycholysis in the 2nd of our cases. Photoonycholysis has only rarely been reported to result from PUVA [5,6]. Its sudden onset, its rapid disappearance despite the continuation of therapy, and the absence of any local protection of the nails tends to implicate the synthetic quinolone derivative.…”

Section: Commentsmentioning

confidence: 99%