Photoacoustic Calorimetry Studies of O2-Sensing FixL and (R200, I209) Variants from Sinorhizobium meliloti Reveal Conformational Changes Coupled to Ligand Photodissociation from the Heme-PAS Domain
Audrey Mokdad,
EuTchen Ang,
Michael Desciak
et al.
Abstract:FixL is an oxygen-sensing heme-PAS protein that regulates
nitrogen
fixation in the root nodules of plants. In this paper, we present
the first photothermal studies of the full-length wild-type FixL protein
from
Sinorhizobium meliloti
and the
first thermodynamic profile of a full-length heme-PAS protein. Photoacoustic
calorimetry studies reveal a quadriphasic relaxation for
Sm
FixL*WT and the five variant proteins (
Sm
FixL*R200H,
… Show more
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