2023
DOI: 10.1021/acs.biochem.3c00438
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Photoacoustic Calorimetry Studies of O2-Sensing FixL and (R200, I209) Variants from Sinorhizobium meliloti Reveal Conformational Changes Coupled to Ligand Photodissociation from the Heme-PAS Domain

Audrey Mokdad,
EuTchen Ang,
Michael Desciak
et al.

Abstract: FixL is an oxygen-sensing heme-PAS protein that regulates nitrogen fixation in the root nodules of plants. In this paper, we present the first photothermal studies of the full-length wild-type FixL protein from Sinorhizobium meliloti and the first thermodynamic profile of a full-length heme-PAS protein. Photoacoustic calorimetry studies reveal a quadriphasic relaxation for Sm FixL*WT and the five variant proteins ( Sm FixL*R200H, … Show more

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