Photoacoustic imaging enhanced by indocyanine green-conjugated single-wall carbon nanotubes

Zanganeh, Saeid; Li, Hai; Kumavor, Patrick D.; Alqasemi, Umar; Aguirre, Andres; Mohammad, Innus; Stanford, Courtney; Smith, Michael B.; Zhu, Quing

doi:10.1117/1.jbo.18.9.096006

Cited by 59 publications

(43 citation statements)

References 60 publications

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“…172,210,214 SWNT has a diameter of several nm and length of 50–300 nm and ICG was attached to the surface of the SWNT. Hence it is expected that the total particle size can remain relatively small, enabling tumor targeting by iv injection.…”

Section: Organic Compoundsmentioning

confidence: 99%

Structural and functional photoacoustic molecular tomography aided by emerging contrast agents

2014

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Photoacoustic tomography (PAT) can offer structural, functional and molecular contrasts at scalable observation level. By ultrasonically overcoming the strong optical scattering, this imaging technology can reach centimeters penetration depth while retaining high spatial resolution in biological tissue. Recent extensive research has been focused on developing new contrast agents to improve the imaging sensitivity, specificity and efficiency. These emerging materials have substantially accelerated PAT applications in signal sensing, functional imaging, biomarker labeling and therapy monitoring etc. Here, the potentials of different optical probes as PAT contrast agents were elucidated. We first describe the instrumental embodiments and the measured functional parameters, then focus on emerging contrast agent-based PAT applications, and finally discuss the challenges and prospects.

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Section: Organic Compoundsmentioning

confidence: 99%

Structural and functional photoacoustic molecular tomography aided by emerging contrast agents

2014

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“…Several photoacoustic imaging studies utilized various exogenous agents, such as gold nanoparticles (AuNPs) 12,13 and indocyanine green (ICG) 14,15 . AuNPs can be good candidates as contrast agents for photoacoustic imaging due to their excellent biocompatibility and high optical absorption.…”

Section: Introductionmentioning

confidence: 99%

Dual-wavelength optical-resolution photoacoustic microscopy for cells with gold nanoparticle bioconjugates in three-dimensional cultures

et al. 2016

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Three-dimensional (3D) in vitro models bridge the gap between typical two-dimensional cultures and in vivo conditions. However, conventional optical imaging methods such as confocal microscopy and two-photon microscopy cannot accurately depict cellular processing in 3D models due to limited penetration of photons. We developed a dualwavelength optical-resolution photoacoustic microscopy (OR-PAM), which provides sufficient penetration depth and spatial resolution, for studying CD8+ cytotoxic T lymphocytes (CTLs) trafficking in an in vitro 3D tumor microenvironment. CTLs play a cardinal role in host defense against tumor. Efficient trafficking of CTLs to the tumor microenvironment is a critical step for cancer immunotherapy. For the proposed system, gold nanospheres and indocyanine green (ICG) have been remarkable choices for contrast agents for photoacoustic signals due to their excellent biocompatibility and high optical absorption. With distinct absorption spectrums, targeted cells with gold nanospheres and ICG respectively can be identified by switching 523-nm and 800-nm laser irradiation. Moreover, we use an x-y galvanometer scanner to obtain high scanning rate. In the developed system, lateral and axial resolutions were designed at 1.6 µm and 5 µm, respectively. We successfully showed that dual-spectral OR-PAM can map either the distribution of CTLs with gold nanospheres at a visible wavelength of 523 nm or the 3D structure of tumor spheres with ICG in an in vitro 3D microenvironment. Our OR-PAM can provide better biological relevant information in cellular interaction and is potential for preclinical screening of anti-cancer drugs.

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“…12 One of the oldest and best studied PAI contrast agents is the FDA-approved ocular angiographic dye indocyanine green (ICG, 3 ), and related derivatives. 13 ICG possesses NIR absorption (λ max ~ 800 nm) properties but is otherwise far from ideal. For instance, ICG is fluorescent.…”

Section: Introductionmentioning

confidence: 99%

In vivo photoacoustic tumor tomography using a quinoline-annulated porphyrin as NIR molecular contrast agent

et al. 2017

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The synthesis and photophysical properties of a tetra-PEG-modified and freely water-soluble quinoline-annulated porphyrin are described. We previously demonstrated the ability of quinoline-annulated porphyrins to act as an in vitro NIR photoacoustic imaging (PAI) contrast agent. The solubility of the quinoline-annulated porphyrin derivative in serum now allowed the assessment of the efficacy of the PEGylated derivative as an in vivo NIR contrast agent for the PAI of an implanted tumor in a mouse model. A multi-fold contrast enhancement when compared to the benchmark dye ICG could be shown, a finding that could be traced to its photophysical properties (short triplet lifetimes, low fluorescence and singlet oxygen sensitization quantum yields). A NIR excitation wavelength of 790 nm could be used, fully taking advantage of the optical window of tissue. Rapid renal clearance of the dye was observed. Its straight-forward synthesis, optical properties with the possibility for further optical fine-tuning, nontoxicity, favorable elimination rates, and contrast enhancement make this a promising PAI contrast agent. The ability to conjugate the PAI chromophore with a fluorescent tag using a facile and general conjugation strategy was also demonstrated.

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Photoacoustic imaging enhanced by indocyanine green-conjugated single-wall carbon nanotubes

Cited by 59 publications

References 60 publications

Structural and functional photoacoustic molecular tomography aided by emerging contrast agents

Structural and functional photoacoustic molecular tomography aided by emerging contrast agents

Dual-wavelength optical-resolution photoacoustic microscopy for cells with gold nanoparticle bioconjugates in three-dimensional cultures

In vivo photoacoustic tumor tomography using a quinoline-annulated porphyrin as NIR molecular contrast agent

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