Photoacoustic Imaging in Gastroenterology: Advances and Needs

Bhushan, Sheena; Anandasabapathy, Sharmila; Петрова, Е. В.

doi:10.5772/intechopen.86051

Cited by 3 publications

(5 citation statements)

References 54 publications

(56 reference statements)

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“…We believe that these changes may become even more pronounced through intravenous delivery of the therapies, as oral delivery may not produce as drastic volumetric reductions as anticipated and seen in previous studies ( 76 ). Furthermore, recent developments in clinical translation of PAI ( 77 – 79 ) including portable real-time LED-based PAI systems for cancer applications ( 80 ) and endoscopic PAI systems ( 81 – 83 ) show promise toward employing photoacoustic monitoring of tumor response to treatment not only for pancreatic tumors but also for other solid tumors undergoing neoadjuvant treatment. Overall, given the exponential rise in the technical advances and biological applications of PAI over the past decade, the results presented in this study further support its utility as a useful tool to monitor cancer treatment response, especially in imaging response of suboptimal therapies.…”

Section: Discussionmentioning

confidence: 99%

3D Ultrasound-Guided Photoacoustic Imaging to Monitor the Effects of Suboptimal Tyrosine Kinase Inhibitor Therapy in Pancreatic Tumors

et al. 2022

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Pancreatic cancer is a disease with an incredibly poor survival rate. As only about 20% of patients are eligible for surgical resection, neoadjuvant treatments that can relieve symptoms and shrink tumors for surgical resection become critical. Many forms of treatments rely on increased vulnerability of cancerous cells, but tumors or regions within the tumors that may be hypoxic could be drug resistant. Particularly for neoadjuvant therapies such as the tyrosine kinase inhibitors utilized to shrink tumors, it is critical to monitor changes in vascular function and hypoxia to predict treatment efficacy. Current clinical imaging modalities used to obtain structural and functional information regarding hypoxia or oxygen saturation (StO2) do not provide sufficient depth penetration or require the use of exogenous contrast agents. Recently, ultrasound-guided photoacoustic imaging (US-PAI) has garnered significant popularity, as it can noninvasively provide multiparametric information on tumor vasculature and function without the need for contrast agents. Here, we built upon existing literature on US-PAI and demonstrate the importance of changes in StO2 values to predict treatment response, particularly tumor growth rate, when the outcomes are suboptimal. Specifically, we image xenograft mouse models of pancreatic adenocarcinoma treated with suboptimal doses of a tyrosine kinase inhibitor cabozantinib. We utilize the US-PAI data to develop a multivariate regression model that demonstrates that a therapy-induced reduction in tumor growth rate can be predicted with 100% positive predictive power and a moderate (58.33%) negative predictive power when a combination of pretreatment tumor volume and changes in StO2 values pretreatment and immediately posttreatment was employed. Overall, our study indicates that US-PAI has the potential to provide label-free surrogate imaging biomarkers that can predict tumor growth rate in suboptimal therapy.

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Section: Discussionmentioning

confidence: 99%

3D Ultrasound-Guided Photoacoustic Imaging to Monitor the Effects of Suboptimal Tyrosine Kinase Inhibitor Therapy in Pancreatic Tumors

et al. 2022

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“…The choice of diagnostic modality depends on many factors, such as the underlying pathological condition, the tissue/organ, and the intended application. For example, optical and photoacoustic imaging are suitable for studying superficial structures, endoscopy is a powerful tool for examining the walls and internal organs of the gastrointestinal tract and digestive diseases [181], non-invasive MRI, PET, and CT can be applied for whole-body imaging [182], while ultrasound is a rapid and low-cost method of examining the body's internal organs [183].…”

Section: In Vivo Applications Of Peptide-based Nanoassemblies As Probmentioning

confidence: 99%

“…Superfibril formation through co-assembly of racemic mixtures (Mito-Rac) was observed inside mitochondria, resulting in disrupted mitochondria both in vitro and in tumor xenografts [192]. In Another NIR dye-labeled peptide (P18-PLGVRGRGD) forming nanofibers at tumor sites was used in photoacoustic imaging (PA) [181,[189][190][191]. This peptide comprised an RGD for tumor targeting and a sequence (PLGVRG) that was cleaved by gelatinase overexpressed in the tumor microenvironment.…”

Section: Nanofibers Improve the Accumulation Of Contrast Agentsmentioning

confidence: 99%

“…Another NIR dye-labeled peptide (P18-PLGVRGRGD) forming nanofibers at tumor sites was used in photoacoustic imaging (PA) [ 181 , 189 , 190 , 191 ]. This peptide comprised an RGD for tumor targeting and a sequence (PLGVRG) that was cleaved by gelatinase overexpressed in the tumor microenvironment.…”

Section: In Vivo Applications Of Peptide-based Nanoassemblies As Pmentioning

confidence: 99%

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Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

et al. 2020

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Nanotechnology approaches play an important role in developing novel and efficient carriers for biomedical applications. Peptides are particularly appealing to generate such nanocarriers because they can be rationally designed to serve as building blocks for self-assembling nanoscale structures with great potential as therapeutic or diagnostic delivery vehicles. In this review, we describe peptide-based nanoassemblies and highlight features that make them particularly attractive for the delivery of nucleic acids to host cells or improve the specificity and sensitivity of probes in diagnostic imaging. We outline the current state in the design of peptides and peptide-conjugates and the paradigms of their self-assembly into well-defined nanostructures, as well as the co-assembly of nucleic acids to form less structured nanoparticles. Various recent examples of engineered peptides and peptide-conjugates promoting self-assembly and providing the structures with wanted functionalities are presented. The advantages of peptides are not only their biocompatibility and biodegradability, but the possibility of sheer limitless combinations and modifications of amino acid residues to induce the assembly of modular, multiplexed delivery systems. Moreover, functions that nature encoded in peptides, such as their ability to target molecular recognition sites, can be emulated repeatedly in nanoassemblies. Finally, we present recent examples where self-assembled peptide-based assemblies with “smart” activity are used in vivo. Gene delivery and diagnostic imaging in mouse tumor models exemplify the great potential of peptide nanoassemblies for future clinical applications.

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“…In recent years, in-vivo optical microscopy techniques, such as confocal laser endomicroscopy (CLE) and high-resolution microendoscopy (HRME), have been applied to visualize the nuclear morphology of the esophageal epithelium and assist in differentiating neoplasia from benign tissue. 3 , 5 – 7 In contrast to conventional tissue biopsies and histopathologic analysis, these optical techniques are non-invasive and able to provide real-time results. However, microendoscopy is currently limited by its small FOV (the average diameter of a probe is 0.3 mm) that hinders it from imaging larger tissue areas.…”

Section: Introductionmentioning

confidence: 99%

Deep-learning-based image super-resolution of an end-expandable optical fiber probe for application in esophageal cancer diagnostics

Zhang,

Tan,

Nabil

et al. 2024

J. Biomed. Opt.

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Endoscopic screening for esophageal cancer (EC) may enable early cancer diagnosis and treatment. While optical microendoscopic technology has shown promise in improving specificity, the limited field of view (<1 mm) significantly reduces the ability to survey large areas efficiently in EC screening.Aim: To improve the efficiency of endoscopic screening, we propose a novel concept of end-expandable endoscopic optical fiber probe for larger field of visualization and for the first time evaluate a deep-learning-based image super-resolution (DL-SR) method to overcome the issue of limited sampling capability.Approach: To demonstrate feasibility of the end-expandable optical fiber probe, DL-SR was applied on simulated low-resolution microendoscopic images to generate super-resolved (SR) ones. Varying the degradation model of image data acquisition, we identified the optimal parameters for optical fiber probe prototyping. The proposed screening method was validated with a human pathology reading study.Results: For various degradation parameters considered, the DL-SR method demonstrated different levels of improvement of traditional measures of image quality. The endoscopists' interpretations of the SR images were comparable to those performed on the high-resolution ones.Conclusions: This work suggests avenues for development of DL-SR-enabled sparse image reconstruction to improve high-yield EC screening and similar clinical applications.

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Photoacoustic Imaging in Gastroenterology: Advances and Needs

Cited by 3 publications

References 54 publications

3D Ultrasound-Guided Photoacoustic Imaging to Monitor the Effects of Suboptimal Tyrosine Kinase Inhibitor Therapy in Pancreatic Tumors

3D Ultrasound-Guided Photoacoustic Imaging to Monitor the Effects of Suboptimal Tyrosine Kinase Inhibitor Therapy in Pancreatic Tumors

Peptide-Based Nanoassemblies in Gene Therapy and Diagnosis: Paving the Way for Clinical Application

Deep-learning-based image super-resolution of an end-expandable optical fiber probe for application in esophageal cancer diagnostics

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