Photoacoustic imaging of carotid artery atherosclerosis

Kruizinga, Pieter; Steen, Antonius F.W. van der; Jong, Nico de; Springeling, Geert; Robertus, Jan Lukas; Lugt, Aad van der; Soest, Gijs van

doi:10.1117/1.jbo.19.11.110504

Cited by 64 publications

(58 citation statements)

References 17 publications

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“…19 In these systems, the photoacoustic waves are detected from the outside, and minimally invasive or noninvasive procedures are performed by exploiting body cavities such as the esophagus, the nasal cavity, and the rectum. 17,18,[20][21][22] However, the previous internal illumination designs rely on light radiating either from the bare fiber tip or from a small slot on the side of the fiber jacket, which cannot homogeneously illuminate the surrounding tissue. In addition, none of the aforementioned designs have been validated for imaging targets in vivo at depths of more than 5 cm.…”

Section: Introductionmentioning

confidence: 99%

Internal-illumination photoacoustic computed tomography

Lan

Liu

et al. 2018

J. Biomed. Opt.

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We report a photoacoustic computed tomography (PACT) system using a customized optical fiber with a cylindrical diffuser to internally illuminate deep targets. The traditional external light illumination in PACT usually limits the penetration depth to a few centimeters from the tissue surface, mainly due to strong optical attenuation along the light propagation path from the outside in. By contrast, internal light illumination, with external ultrasound detection, can potentially detect much deeper targets. Different from previous internal illumination PACT implementations using forward-looking optical fibers, our internal-illumination PACT system uses a customized optical fiber with a 3-cm-long conoid needle diffuser attached to the fiber tip, which can homogeneously illuminate the surrounding space and substantially enlarge the field of view. We characterized the internal illumination distribution and PACT system performance. We performed tissue phantom and in vivo animal studies to further demonstrate the superior imaging depth using internal illumination over external illumination. We imaged a 7.5-cm-deep leaf target embedded in optically scattering medium and the beating heart of a mouse overlaid with 3.7-cm-thick chicken tissue. Our results have collectively demonstrated that the internal light illumination combined with external ultrasound detection might be a useful strategy to improve the penetration depth of PACT in imaging deep organs of large animals and humans.

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Section: Introductionmentioning

confidence: 99%

Internal-illumination photoacoustic computed tomography

Lan

Liu

et al. 2018

J. Biomed. Opt.

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“…The spectroscopic differentiation capacity is needed for identification of other carotid artery disease biomarkers, as well as extrinsically administered targeted or activatable labels (15). Efforts in carotid plaque imaging are also geared toward noninvasive detection of vulnerable lipid-rich plaques (17,24). In this context, optoacoustic lipid detection at 1200 nm has already been demonstrated ex vivo and in preclinical intravascular studies in vivo (16,17,25).…”

Section: Discussionmentioning

confidence: 99%

“…Efforts in carotid plaque imaging are also geared toward noninvasive detection of vulnerable lipid-rich plaques (17,24). In this context, optoacoustic lipid detection at 1200 nm has already been demonstrated ex vivo and in preclinical intravascular studies in vivo (16,17,25). Thus, the suggested handheld volumetric MSOT approach holds promise most patients.…”

Section: Discussionmentioning

confidence: 99%

“…MSOT imaging of matrix metalloproteinase activity, typically associated with atherosclerotic plaque instability, has been demonstrated in ex vivo human carotid plaque samples (15). By using excitation in the wavelength range of 1210 nm, corresponding to the peak optical absorption by lipids, optoacoustic imaging could clearly identify and characterize plaques within human aorta and carotid artery samples ex vivo (16,17).…”

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confidence: 99%

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Real-time Volumetric Assessment of the Human Carotid Artery: Handheld Multispectral Optoacoustic Tomography

Ivanković¹,

Merčep²,

Schmedt³

et al. 2019

Radiology

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Radiology 2019; 00:1-7 • https://doi.org/10.1148/radiol.2019181325 • Content code:Background: Multispectral optical imaging has the capability of resolving hemoglobin, lipid, and water. Volumetric multispectral optoacoustic tomography (MSOT) is a hybrid imaging technique that provides a unique combination of functional and molecular contrast with real-time handheld imaging.Purpose: To investigate whether volumetric MSOT can provide real-time assessment of the anatomic and functional status of the human carotid artery bifurcation noninvasively. Materials and Methods:Imaging of healthy volunteers (n = 16) was performed with a custom-designed handheld volumetric MSOT scanner capable of high-spatial-resolution (approximately 200 µm) and real-time (10 volumes/sec) three-dimensional imaging, while further providing spectroscopic capacity through fast tuning of the excitation light wavelength. For comparison and anatomic cross-validation, volunteers were also scanned with clinical B-mode US.Results: Volumetric MSOT achieved real-time imaging and characterization of the entire carotid bifurcation area across three dimensions simultaneously captured in a single volumetric image frame. Analysis of the acquired data further showed that a higher contrast-to-noise ratio can be achieved for wavelengths corresponding to a high optical absorption of oxygenated hemoglobin. Conclusion:The human carotid artery was visualized by using handheld volumetric multispectral optoacoustic tomography. This imaging approach is less prone to motion artifacts than are the conventional clinical imaging methods, holding promise for providing additional image-based biomarkers for noninvasive label-free assessment of carotid artery disease.

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“…A few studies have used PAI to investigate atherosclerosis plaques [17,18] as well as to detect breast tumors [19] and melanoma [20]. Thus, PAI not only resolves anatomical contrasts but can also visualize hemoglobin [6,21,22,23], melanin [20], lipids [17,18], and a wide variety of optical agents [24,25,26,27,28]. It can be used to visualize molecular tissue changes and anatomical tissue changes, such as those caused by stenosis.…”

Section: Discussionmentioning

confidence: 99%

Photoacoustic Imaging: A Novel Tool for Detecting Carotid Artery Thrombosis in Mice

Cong

et al. 2017

J Vasc Res

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Thrombosis is a main cause of acute cardiovascular events, and detecting thrombi in small arteries via noninvasive imaging remains challenging. In this study, we employed a novel imaging method, photoacoustic imaging (PAI), to study thrombosis in a mouse model of ferric chloride (FeCl₃)-induced arterial thrombosis and compared the ability of this method to detect thrombosis with that of a conventional imaging method, namely, ultrasound. The mice (n = 20) were divided equally into the following 4 groups: (1) a normal group, and (2) 3 experimental groups, in which the left common carotid artery was treated with 20% FeCl₃ for 1, 3, or 5 min, respectively. After 24 h, PAI detected thrombi of different sizes and generated images, enabling us to assess the changes in structure. The results of this study suggest that PAI is a useful, noninvasive visualization tool for investigating the mechanism underlying thrombosis development and is suitable for imaging arterial thrombosis in mouse carotid arteries.

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Photoacoustic imaging of carotid artery atherosclerosis

Cited by 64 publications

References 17 publications

Internal-illumination photoacoustic computed tomography

Internal-illumination photoacoustic computed tomography

Real-time Volumetric Assessment of the Human Carotid Artery: Handheld Multispectral Optoacoustic Tomography

Photoacoustic Imaging: A Novel Tool for Detecting Carotid Artery Thrombosis in Mice

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