Photoacoustic Monitoring of the Viability of Mesenchymal Stem Cells Labeled with Indocyanine Green

Yoo, Ji-Hyun; Yun, Chang-Koo; Bui, Nhat Quang; Oh, Junghwan; Nam, Seung Yun

doi:10.1016/j.irbm.2018.11.001

Cited by 4 publications

(4 citation statements)

References 29 publications

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“…Viability of stem cells was monitored by Yoo et al using ICG due to its’ relatively high optical absorption at 800 nm (NIR) and its ability to clear out of stem cells after cellular death through diffusion [ 60 ]. Continuous assessment of the amount of viable transplanted cells over time is crucial for developing effective stem cell therapies in a clinical setting.…”

Section: Desirable Characteristics Of Optoacoustic Contrast Agents For Stem Cell Trackingmentioning

confidence: 99%

Contrast agents for photoacoustic imaging: a review of stem cell tracking

et al. 2021

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With the advent of stem cell therapy for spinal cord injuries, stroke, burns, macular degeneration, heart diseases, diabetes, rheumatoid arthritis and osteoarthritis; the need to track the survival, migration pathways, spatial destination and differentiation of transplanted stem cells in a clinical setting has gained increased relevance. Indeed, getting regulatory approval to use these therapies in the clinic depends on biodistribution studies. Although optoacoustic imaging (OAI) or photoacoustic imaging can detect functional information of cell activities in real-time, the selection and application of suitable contrast agents is essential to achieve optimal sensitivity and contrast for sensing at clinically relevant depths and can even provide information about molecular activity. This review explores OAI methodologies in conjunction with the specific application of exogenous contrast agents in comparison to other imaging modalities and describes the properties of exogenous contrast agents for quantitative and qualitative monitoring of stem cells. Specific characteristics such as biocompatibility, the absorption coefficient, and surface functionalization are compared and how the labelling efficiency translates to both short and long-term visualization of mesenchymal stem cells is explored. An overview of novel properties of recently developed optoacoustic contrast agents and their capability to detect disease and recovery progression in clinical settings is provided which includes newly developed exogenous contrast agents to monitor stem cells in real-time for multimodal sensing.

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Section: Desirable Characteristics Of Optoacoustic Contrast Agents For Stem Cell Trackingmentioning

confidence: 99%

Contrast agents for photoacoustic imaging: a review of stem cell tracking

et al. 2021

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“…Indocyanine green (ICG) is an NIR dye that has been used successfully as a contrast agent in both FI and MSOT applications in small animals, [13][14][15] and is FDAapproved for various clinical applications, including the assessment of liver function and the identification of tumour margins during surgery. 16,17 ICG has also been used to label cells and track them in vivo in mice using FI and MSOT.…”

Section: Introductionmentioning

confidence: 99%

“…Near infrared (NIR) nanoprobes that absorb and emit light in the “optical window” (650 nm-900 nm) are particularly suited for tracking cells with both FI and MSOT in vivo because within these wavelengths, there is limited absorbance by haemoglobin. Indocyanine green (ICG) is an NIR dye that has been used successfully as a contrast agent in both FI and MSOT applications in small animals, 13-15 and is FDA-approved for various clinical applications, including the assessment of liver function and the identification of tumour margins during surgery. 16,17 ICG has also been used to label cells and track them in vivo in mice using FI and MSOT.…”

Section: Introductionmentioning

confidence: 99%

Near infrared conjugated polymer nanoparticles (CPN™) for tracking cells using fluorescence and optoacoustic imaging

Muñiz-García

Pichardo

Littlewood

et al. 2023

Preprint

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Tracking the biodistribution of cell therapies is crucial for understanding their safety and efficacy. Optical imaging techniques are particularly useful for tracking cells due to their clinical translatability and potential for intra-operative use to validate cell delivery. However, there is a lack of appropriate optical probes for cell tracking. The only FDA-approved material for clinical use is indocyanine green (ICG). ICG can be used for both fluorescence and photoacoustic imaging, but is prone to photodegradation, and at higher concentrations, undergoes quenching and can adversely affect cell health. We have developed novel near-infrared imaging probes comprising conjugated polymer nanoparticles (CPNs™) that can be fine-tuned to absorb and emit light at specific wavelengths. To compare the performance of the CPNs™ with ICG forin vivocell tracking, labelled mesenchymal stromal cells (MSCs) were injected subcutaneously in mice and detected using fluorescence imaging (FI) and a form of photoacoustic imaging called multispectral optoacoustic tomography (MSOT). MSCs labelled with either ICG or CPN™ 770 could be detected with FI, but only CPN™ 770-labelled MSCs could be detected with MSOT. These results show that CPNs™ show great promise for tracking cellsin vivousing optical imaging techniques, and for some applications, out-perform ICG.

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“…Near infrared (NIR) nanoprobes that absorb and emit light in the "optical window" (650-900 nm) are particularly suited for tracking cells with both FI and MSOT in vivo 13 because within these wavelengths, there is limited absorbance by haemoglobin. Indocyanine green (ICG) is an NIR dye that has been used successfully as a contrast agent in both FI and MSOT applications in small animals, [14][15][16] and is FDA-approved for various clinical applications, including the assessment of liver function and the identication of tumour margins during surgery. 17,18 ICG has also been used to label cells and track them in vivo in mice using FI and MSOT.…”

Section: Introductionmentioning

confidence: 99%